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Immunofluorescence with mouse anti-alpha-tubulin was employed to visualize the details of the microtubule network in a log phase monolayer culture of human osteosarcoma cells. The secondary antibody (goat anti-mouse IgG) was conjugated to Alexa Fluor 488. In addition, mitochondria in the cells were targeted with MitoTracker Red CMXRos, a derivative of X-rosamine.
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