Soluções para ciências da vida
Madin-Darby Canine Kidney Epithelial Cells (MDCK Line)
The close proximity between the Golgi complex and nuclei in Madin-Darby canine kidney cells was probed in a double immunofluorescence experiment with mouse anti-NPCP (nuclear pore complex protein) and rabbit anti-giantin (Golgi complex) primary antibodies. The antibody targets were visualized with goat secondary antibodies conjugated to Alexa Fluor 568 and Alexa Fluor 488, respectively, while the actin cytoskeletal framework was labeled with Alexa Fluor 350 conjugated to phalloidin.
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