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A culture of human osteosarcoma (U-2 OS) cells was stained with Alexa Fluor 594 conjugated to phalloidin and Hoechst 33342, targeting the cytoskeletal filamentous actin network and nuclear DNA, respectively. The cells were also immunofluorescently labeled with primary anti-giantin rabbit monoclonal antibodies followed by goat anti-rabbit secondary antibodies conjugated to Alexa Fluor 488 in order to target the Golgi apparatus.
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