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Modern Slide Scanning: Single-cell Phenotyping on Fixed Samples

Learn about the latest generation of slide scanners in this on-demand webinar. Our experts will discuss how modern slide scanners can be a workhorse for your applications and how multiplexing can reveal much more information from a slide. With a guest expert from Ultivue, you’ll learn how single-cell phenotyping can dramatically improve the understanding of the dynamics in a sample, even if it is a fixed slide.

Presenters:

Alec De Grand, Application Specialist, Life Science Research
Flavio Giacobone, Product Marketing Manager

FAQ

Webinar FAQs | Modern Slide Scanning

Does VS200 slide scanner software align images from different layers automatically?

Yes, there is a tool for automatic image alignment. If you have two layers with one common channel (e.g., DAPI), simply click on the relevant menu item and the tool will do the alignment for you. You also have the option to export data as separate layers to Olympus’ cellSens™ software or other image analysis software.

If you are looking at 8 or 12 different markers, do you still image them in sets of 4?

Yes, when increasing the number of markers, you continue to image them 4 at a time. This way, you can keep using the 4 classical fluorophores: FITC, TRITC, Cy5 and Cy7. This approach avoids bleed-through or crosstalk between fluorophores.

Can modern slide scanning be performed on standard glass slides?

Yes, you can use normal glass slides. There are no special sample preparation requirements—the sample just needs to be paraffin-embedded.

How do you reduce tissue autofluorescence and other noise during image acquisition?

We have tested the Ultivue stains and found that they have a very high signal-to-noise ratio and dynamic range, which reduces the effect of autofluorescence. Also, Ultivue stains do not use a secondary antibody, so you avoid background signal due to nonspecific binding. Finally, choosing the right filters helps to remove background signal.

What is the Virtual Z feature in the VS200 slide scanner?

This is the system’s Z-stack feature, which enables you to do volumetric (3D) imaging by combining multiple exposures at different focal planes. The VS200 scanner also has extended focus imaging (EFI), which can flatten this Z-stack into a single 2D image. This feature is particularly useful when you need a fully focused image from a specimen that is not completely flat.

Does the VS200 scanner have barcode recognition?

Yes, the VS200 slide scanner has an integrated barcode reader that helps you simplify data management. The reader can scan a large selection of 1D and 2D barcode types to capture and record the information automatically.


相关产品

研究级全玻片扫描系统

VS200

  • 2倍到100倍全玻片成像的出色图像质量
  • 具有载玻片尺寸和观察方法选择的全面灵活性
  • 从明场到荧光多色标记简单强大的工作流程
  • 从手动明场扫描到AI识别和全自动扫描均可在软件和硬件进行特别配置

Modern Slide Scanning: Single-cell Phenotyping on Fixed Samples

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Webinar: Modern Slide Scanning: Single-cell Phenotyping on Fixed Samples

Learn about the latest generation of slide scanners in this on-demand webinar. Our experts will discuss how modern slide scanners can be a workhorse for your applications and how multiplexing can reveal much more information from a slide. With a guest expert from Ultivue, you’ll learn how single-cell phenotyping can dramatically improve the understanding of the dynamics in a sample, even if it is a fixed slide.

FAQ

Webinar FAQs | Modern Slide Scanning

Does VS200 slide scanner software align images from different layers automatically?

Yes, there is a tool for automatic image alignment. If you have two layers with one common channel (e.g., DAPI), simply click on the relevant menu item and the tool will do the alignment for you. You also have the option to export data as separate layers to Olympus’ cellSens™ software or other image analysis software.

If you are looking at 8 or 12 different markers, do you still image them in sets of 4?

Yes, when increasing the number of markers, you continue to image them 4 at a time. This way, you can keep using the 4 classical fluorophores: FITC, TRITC, Cy5 and Cy7. This approach avoids bleed-through or crosstalk between fluorophores.

Can modern slide scanning be performed on standard glass slides?

Yes, you can use normal glass slides. There are no special sample preparation requirements—the sample just needs to be paraffin-embedded.

How do you reduce tissue autofluorescence and other noise during image acquisition?

We have tested the Ultivue stains and found that they have a very high signal-to-noise ratio and dynamic range, which reduces the effect of autofluorescence. Also, Ultivue stains do not use a secondary antibody, so you avoid background signal due to nonspecific binding. Finally, choosing the right filters helps to remove background signal.

What is the Virtual Z feature in the VS200 slide scanner?

This is the system’s Z-stack feature, which enables you to do volumetric (3D) imaging by combining multiple exposures at different focal planes. The VS200 scanner also has extended focus imaging (EFI), which can flatten this Z-stack into a single 2D image. This feature is particularly useful when you need a fully focused image from a specimen that is not completely flat.

Does the VS200 scanner have barcode recognition?

Yes, the VS200 slide scanner has an integrated barcode reader that helps you simplify data management. The reader can scan a large selection of 1D and 2D barcode types to capture and record the information automatically.


相关产品

研究级全玻片扫描系统

VS200

  • 2倍到100倍全玻片成像的出色图像质量
  • 具有载玻片尺寸和观察方法选择的全面灵活性
  • 从明场到荧光多色标记简单强大的工作流程
  • 从手动明场扫描到AI识别和全自动扫描均可在软件和硬件进行特别配置
Experts
Alec De Grand
产品经理

我叫Alec De Grand,是奥林巴斯虚拟载玻片扫描仪和正置显微镜的产品经理。我在奥林巴斯就职超过10多年,曾负责管理过临床产品、营销计划、显微成像课程和贸易展会。

Flavio Giacobone
产品营销经理

大家好,我叫Flavio Giacobone,负责奥林巴斯欧洲市场的全玻片扫描解决方案。从攻读生物医学工程专业学位开始,成像始终与我息息相关,加入奥林巴斯让我能够亲身体验到很多临床和科研领域的数字化转变。我从奥林巴斯第一台扫描仪dotSlide开始,就在整个玻片成像方面积累了专业知识,见证了自第一款产品以来所取得的巨大进步。

Modern Slide Scanning: Single-cell Phenotyping on Fixed Samples2021年10月22日
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