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Imaging the Mitochondrial Network in Monolayer 3T3 Fibroblast Cell Cultures

Imaging the Mitochondrial Network in Monolayer 3T3 Fibroblast Cell Cultures

A monolayer culture of Swiss mouse embryo cells was treated with MitoTracker Red CMXRos in medium containing 15 percent Cosmic calf serum, fixed with the same medium containing 3.7 percent paraformaldehyde, permeabilized with 0.2 percent Triton X-100, and then counterstained with SYTOX Green, targeting DNA in the nuclei.

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