Madin-Darby Ovine Kidney Cells Stained with Alexa Fluor Dyes and TO-PRO-3 | Olympus LS

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Madin-Darby Ovine Kidney Cells Stained with Alexa Fluor Dyes and TO-PRO-3 — Madin-Darby Ovine Kidney Cells Stained with Alexa Fluor Dyes and TO-PRO-3
A culture of MDOK cells was labeled with TO-PRO-3 (pseudocolored blue), which exhibits affinity for DNA in the cell nucleus, and Alexa Fluor 594 conjugated to phalloidin, which binds to the cytoskeletal filamentous actin network. The epithelial cell culture was also probed immunofluorescently with primary anti-oxphos complex V inhibitor protein monoclonal antibodies followed by goat anti-mouse Fab fragments conjugated to Alexa Fluor 488.

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