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The Metaphase Spindle in Dividing Indian Muntjac Cells

The Metaphase Spindle in Dividing Indian Muntjac Cells

The metaphase spindle apparatus is revealed in this confocal image of Indian Muntjac deer skin cells stained with Alexa Fluor 568 for microtubules and TO-PRO-3 for DNA. The cells were fixed with 3.7 percent paraformaldehyde, permeabilized with Triton X-100, blocked with 10 percent normal goat serum and treated with mouse anti-alpha-tubulin primary antibodies. Goat anti-mouse secondary antibodies conjugated to Alexa Fluor 568 were mixed with the DNA stain.

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