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Human Brain Glioma Cells (U-118 MG Line)

Human Brain Glioma Cells (U-118 MG Line)

In a double immunofluorescence experiment, an adherent monolayer culture of U-118 MG cells was fixed, permeabilized, blocked with 10 percent normal goat serum, and treated with a cocktail of mouse anti-histones (pan) and rabbit anti-PMP 70 (peroxisomal membrane protein) primary antibodies, followed by goat anti-mouse and anti-rabbit secondary antibodies (IgG) conjugated to Texas Red and Alexa Fluor 488, respectively. The filamentous actin network was counterstained with Alexa Fluor 350 conjugated to phalloidin.

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