Targeting the Microtubule Network in Mouse Endothelial Cells with Immunofluorescence
The EOMA endothelial cell shown in this confocal image was resident in a culture that was fixed, permeabilized, blocked, and then treated with mouse primary antibodies that target alpha-tubulin, a major component of the microtubule network. The primaries were labeled with goat anti-mouse secondary antibodies conjugated to Alexa Fluor 555 in a solution containing TO-PRO-3 to simultaneously stain DNA.
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