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Targeting the Microtubule Network in Mouse Endothelial Cells with Immunofluorescence

Targeting the Microtubule Network in Mouse Endothelial Cells with Immunofluorescence

The EOMA endothelial cell shown in this confocal image was resident in a culture that was fixed, permeabilized, blocked, and then treated with mouse primary antibodies that target alpha-tubulin, a major component of the microtubule network. The primaries were labeled with goat anti-mouse secondary antibodies conjugated to Alexa Fluor 555 in a solution containing TO-PRO-3 to simultaneously stain DNA.

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