Gerbil Lung Cells Labeled for Tubulin with Immunofluorescence
In order to visualize the microtubules present in the gerbil lung cells shown in this confocal image, a GeLu culture was immunofluorescently labeled with anti-tubulin mouse monoclonal primary antibodies followed by goat anti-mouse Fab fragments conjugated to Alexa Fluor 488. In addition, the cells were labeled for the cytoskeletal F-actin network with Alexa Fluor 546 (pseudocolored blue) conjugated to phalloidin, and for cell nuclei with TO-PRO-3.
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