Human Lung Carcinoma Cells (A-549 Line)
This section features a culture of human lung carcinoma cells that was transfected with a pEYFP-Mitochondria plasmid subcellular localization vector, which contains the mitochondrial targeting sequence from subunit VIII of human cytochrome C oxidase. The enhanced yellow fluorescent protein gene used with this culture contains several important amino acid substitutions that shift the emission maximum of green fluorescent protein (GFP) by approximately 18 nanometers, from 509 to 527 nanometers. The cells were additionally labeled with the nucleic acid stain SYTOX Orange and Alexa Fluor 350 conjugated to phalloidin, targeting DNA and filamentous actin, respectively.
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