Mongoose Skin Fibroblast Cells (APM)
In a triple-fluorophore experiment similar to the one described in the previous section, a culture of African water mongoose cells was fixed, permeabilized, blocked with 10-percent normal goat serum in PBS, and then treated with mouse anti-alpha-tubulin primary antibodies. The secondary antibody (goat anti-mouse IgG) was conjugated to Oregon Green 488 and mixed with Alexa Fluor 594 conjugated to phalloidin. Nuclei were again counterstained with Hoechst 33258.
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