Tahr Ovary Epithelial Cells (HJ1.Ov Line)
After treatment for one hour with MitoTracker Red CMXRos in serum-based growth medium, an adherent culture of HJ1.Ov cells was fixed in 50:50 methanol:acetone, permeabilized, blocked, and treated with mouse anti-cytokeratin (pan) monoclonal antibodies. The intermediate filaments were visualized with goat anti-mouse secondary antibodies (IgG) conjugated to Marina Blue, and the nuclei were counterstained with SYTOX Green.
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