Madin-Darby Ovine Kidney Epithelial Cells (MDOK Line)
An adherent monolayer culture of MDOK cells was fixed, permeabilized, and treated with a mixture of concanavalin A conjugated to Alexa Fluor 350 and phalloidin conjugated to Alexa Fluor 488 to target the endoplasmic reticulum and filamentous actin network. After washing, the cells were counterstained with SYTOX Orange to visualize the nuclei.
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