Male Rat Kangaroo Kidney Epithelial Cells (PtK2)
In a manner similar to the previous culture, the extensive microtubular network present in PtK2 cells was immunofluorescently labeled with primary anti-tubulin mouse monoclonal antibodies followed by goat anti-mouse Fab fragments conjugated to Cy3. The epithelial cells were also stained with DAPI, which preferentially binds to nuclear DNA.
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