Human Bone Osteosarcoma Cells (U-2 OS Line)
A healthy log-phase culture of human osteosarcoma cells was fixed, permeabilized, and blocked with 10-percent normal goat serum in phosphate-buffered saline prior to immunofluorescent labeling with rabbit primary antibodies to giantin. The culture was subsequently stained with a mixture of goat anti-rabbit secondary antibodies conjugated to Alexa Fluor 488, in order to visualize the giantin, and Alexa Fluor 568 conjugated to phalloidin for the filamentous actin cytoskeleton. Hoechst 33342 was used to visualize the nuclei.
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