Human Bone Osteosarcoma Cells (U-2 OS Line)
A culture of human osteosarcoma cells was transfected with a pDsRed-Mitochondria plasmid subcellular localization vector, thus localizing a red fluorescent protein tag to the intracellular mitochondrial network. After isolation of stable transfectants, a healthy culture was fixed and subsequently labeled with DAPI and Alexa Fluor 488 conjugated to phalloidin, targeting DNA and F-actin, respectively.
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