Visualizing Intermediate Filaments and the Golgi Complex in BPAE Cells with Immunofluorescence | Olympus LS

Home/ Learn/ Microscopy Resource Center/ Digital Image Galleries/ confocal/ cells/ bpae/ Visualizing Intermediate Filaments and the Golgi Complex in BPAE Cells with Immunofluorescence

Visualizing Intermediate Filaments and the Golgi Complex in BPAE Cells with Immunofluorescence — Visualizing Intermediate Filaments and the Golgi Complex in BPAE Cells with Immunofluorescence
In a double immunofluorescence experiment, an adherent monolayer culture of BPAE cells was fixed, permeabilized, blocked with 10-percent normal goat serum, and then treated with a cocktail of mouse anti-vimentin (intermediate filaments) and rabbit anti-GPP130 (Golgi complex) primary antibodies followed by goat anti-mouse and anti-rabbit secondary antibodies (IgG) conjugated to Alexa Fluor 568 (pseudocolored blue) and Alexa Fluor 488, respectively.

Sorry, this page is not
available in your country.

Sorry, this page is not available in your country