Overview

	Next Generation FLUOVIEW for the Next Revolutions in Science The FLUOVIEW FV3000 series of confocal laser scanning microscopes meets some of the most difficult challenges in modern science. Featuring the high sensitivity and speed required for live cell imaging as well as deep tissue observation, the FV3000 confocal microscope enables a wide range of imaging modalities, including macro-to-micro imaging, super resolution microscopy, and quantitative data analysis. This product has been discontinued, check out our current product

Next Generation FLUOVIEW for the Next Revolutions in Science

The FLUOVIEW FV3000 series of confocal laser scanning microscopes meets some of the most difficult challenges in modern science. Featuring the high sensitivity and speed required for live cell imaging as well as deep tissue observation, the FV3000 confocal microscope enables a wide range of imaging modalities, including macro-to-micro imaging, super resolution microscopy, and quantitative data analysis.

This product has been discontinued, check out our current product

High-Sensitivity Image Multiplexing from Violet to NIR

Using proprietary spectral detection technology, the FV3000 confocal microscope's TruSpectral detectors combine high sensitivity with spectral flexibility to detect even the dimmest fluorophores.

TruSpectral technology achieves up to 3X more light transmission vs. traditional spectral detection technology by implementing the volume phase holographic (VPH) diffraction grating.
Offers independently adjustable channels to optimize signal detection for each individual fluorophore. Variable barrier filter mode provides simultaneous four-channel image acquisition with up to sixteen channels in sequential mode.
Lambda scanning mode enables accurate spectral unmixing of complex overlapping fluorescent signals.

Our new FV3000 Red near-infrared (NIR) solution further extends the FV3000 microscope’s wavelength detection capabilities to the NIR region of up to 890 nm through a suite of carefully engineered NIR upgrades:

730 nm or 785 nm lasers and an NIR-sensitive 1- or 2-channel GaAs detectors enable up to 6 channels for multiplexed imaging from violet to NIR (400 nm–890 nm).
TruFocus Red enables stable and gentle NIR time-lapse imaging.
X Line objectives offer the widest range of chromatic correction, from 400 nm – 1000 nm, for exceptional color accuracy during multicolor fluorescence imaging

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_{(a) Mouse mPFC labeled with glial fibrillary acidic protein (GFAP; astrocyte marker; yellow), calmodulin-dependent protein kinase II (CaMKII; pyramidal neuron marker; red), amphoterin-induced protein 1 precursor (AMIGO-1; neuronal membrane marker; cyan), parvalbumin (PV; inhibitory neuron marker; purple), ankyrin-G (AnkG; axon initial segment marker; green), and nuclear yellow (nuclei marker; blue).

Image data courtesy of Stephanie Shiers, Ph.D. Candidate, and Theodore J. Price, Ph.D., Price Lab, Eugene McDermott Professor, Director, Center for Advanced Pain Studies, Department of Neurobiology, School of Behavioral and Brain Sciences, University of Texas at Dallas.

(b) Rat brain slice labeled with Hoechst (blue), anti-IBA1 (Alexa Fluor 488; green), anti-MAP2 (Alexa Fluor 594, yellow), anti-FOX3/NeuN (Alexa Fluor 647; red), and anti-MBP (Alexa Fluor 750; cyan). Images were acquired using a UPLXAPO10X objective with 405 nm, 488 nm, 561 nm, and 730 nm laser lines on GaAsP and GaAs detectors. Maximum intensity projection in Z with TruSight deconvolution processing.

Sample courtesy of EnCor Biotechnology.}

Macro-to-Micro Imaging and Super Resolution Microscopy

The FV3000 microscope’s macro-to-micro workflow provides a roadmap for data acquisition, enabling you to see data in context and easily locate regions of interest for higher resolution imaging.

Use a low-magnification 1.25X or 2X objective to quickly capture a large field of view (FOV) map of whole specimens
Identify regions of interest on the Overlay Map, then switch to a higher magnification objective for high-resolution confocal imaging down to 120 nm with Olympus Super Resolution technology (FV-OSR)
Finalize your acquisition and get publication-ready microscope images with TruSight image processing

Learn more

Macro-to-Micro Imaging and Super Resolution Microscopy

Mouse brain hemisection embedded for expansion microscopy (pre-expansion), labeled with secondary antibodies against GFP (Alexa Fluor 488, green), SV2 (Alexa Fluor 565, red), Homer (Alexa Fluor 647, blue).
Sample courtesy of Dr. Ed Boyden and Dr. Fei Chen, MIT.

Macro-to-Micro Imaging and Super Resolution Microscopy

Dendrite (anti-GFP Alexa Fluor 488, green) and synaptic marker (SV2, Alexa Fluor 565, red). Olympus Super Resolution image processed with cellSens advanced constrained iterative deconvolution. Average full width half maximum measurements ~135 nm. Image acquired with 100X 1.35 NA silicone objective.
Sample courtesy of Dr. Ed Boyden and Dr. Fei Chen, MIT.

Hybrid Scanning for High-Speed Imaging and Increased Productivity The FV3000 Hybrid Scanner provides two scanners in one for enhanced confocal imaging capabilities. The FV3000RS hybrid scan unit uses a galvanometer scanner for precision scanning as well as a resonant scanner, ideal for high-speed imaging of live physiological events Capture video-rate images with a large FOV using the resonant scanner, featuring speeds from 30 frames per second (fps) at FN 18 all the way up to 438 fps using clip scanning Use the resonant scanner to observe fast phenomena, such as a beating heart, blood flow, or calcium ion (Ca2+) dynamics inside cells Switch between the galvanometer scanner and resonant scanner with the click of a button Learn more	Related Videos

Hybrid Scanning for High-Speed Imaging and Increased Productivity

The FV3000 Hybrid Scanner provides two scanners in one for enhanced confocal imaging capabilities.

The FV3000RS hybrid scan unit uses a galvanometer scanner for precision scanning as well as a resonant scanner, ideal for high-speed imaging of live physiological events
Capture video-rate images with a large FOV using the resonant scanner, featuring speeds from 30 frames per second (fps) at FN 18 all the way up to 438 fps using clip scanning
Use the resonant scanner to observe fast phenomena, such as a beating heart, blood flow, or calcium ion (Ca2+) dynamics inside cells
Switch between the galvanometer scanner and resonant scanner with the click of a button

Learn more

Related Videos	Accurate Time-Lapse Imaging Time-lapse experiments require consistent focus and low phototoxicity to the sample. Olympus’ TruFocus unit helps maintain focus during live cell imaging despite changes in temperature or added reagents The FV3000 microscope’s high-sensitivity detector requires significantly less laser power while the resonant scanner reduces laser illumination time, lowering phototoxicity for more physiologically accurate confocal imaging data Learn more

Accurate Time-Lapse Imaging

Time-lapse experiments require consistent focus and low phototoxicity to the sample.

Olympus’ TruFocus unit helps maintain focus during live cell imaging despite changes in temperature or added reagents
The FV3000 microscope’s high-sensitivity detector requires significantly less laser power while the resonant scanner reduces laser illumination time, lowering phototoxicity for more physiologically accurate confocal imaging data

Learn more

Deep Tissue Observation with Silicone Objectives The refractive index of silicone oil is close to that of living tissue, enabling high-resolution observation deep inside tissue with minimal spherical aberration. Refractive index match delivers an ideal focal volume, resulting in perfect volume reconstruction and enabling high-resolution confocal imaging of large living organisms Long working distances enable detailed microscopic imaging at depth See data unfold in real-time and easily observe structures with 3D reconstruction software Learn more	_{3D image of chick ciliary ganglion cleared by a tissue clearing reagent. Image data courtesy of Dr. Ryo Egawa. Tohoku University Graduate School of Life Science.}

Deep Tissue Observation with Silicone Objectives

The refractive index of silicone oil is close to that of living tissue, enabling high-resolution observation deep inside tissue with minimal spherical aberration.

Refractive index match delivers an ideal focal volume, resulting in perfect volume reconstruction and enabling high-resolution confocal imaging of large living organisms
Long working distances enable detailed microscopic imaging at depth
See data unfold in real-time and easily observe structures with 3D reconstruction software

Learn more

_{3D image of chick ciliary ganglion cleared by a tissue clearing reagent.

Image data courtesy of Dr. Ryo Egawa. Tohoku University Graduate School of Life Science.}

Automated Organoid Imaging

From finding target objects to capturing high-resolution 3D images, the workflow for imaging organoids can be highly time consuming, particularly when imaging multiple wells across a microplate. The Macro-to-Micro module for the FV3000 system is a smart solution that automates the organoid imaging workflow to dramatically increase imaging efficiency.

The FV3000 microscope captures images at low magnification, and then the Macro-to-Micro software module can automatically locate your objects of interest in the vessel or well and capture them at high magnification. This automated process dramatically reduces the time you spend on microscope operation.

Learn more

Automated Macro-to-Micro Imaging

Applied Technologies

TruSpectral Detection

Groundbreaking TruSpectral detection technology offers outstanding results compared to previous generations of spectral detection units. Featured on every channel in the FV3000 microscope, TruSpectral detection technology combines the flexibility of a spectral detector with the sensitivity of a filter-based detector.

Sensitivity and Accuracy

Enhanced Quantum Efficiency

How TruSpectral Detection Technology Works

Based on patented volume phase hologram (VPH) transmission for high efficiency, TruSpectal detection uses an adjustable slit to select the detection wavelengths of each individual channel to 2 nm.

Sensitivity and Accuracy

Integrated in all FV3000 confocal microscopes, TruSpectral detection technology enables much higher light throughput compared to conventional spectral detection units. The volume phase hologram diffracts light with up to three-fold higher transmission efficiency vs. reflection gratings. The result is excellent multicolor fluorescence microscopy images of live and fixed tissue.

Enhanced Quantum Efficiency

The FV3000 microscope's high-sensitivity detector (HSD) enables you to view samples whose emission is too weak to view with conventional detectors. The HSD unit incorporates two GaAsP channels with a maximum quantum efficiency of 45% and Peltier cooling that reduces background noise by 20% for high signal-to-noise (S/N) ratio images under very low excitation light. HSD units can be combined to enable four-channel GaAsP imaging with the FV3000 system.

. ,	Multichannel TruSpectral Detection with Sixteen-Channel Unmixing TruSpectral detection works independently on all microscope channels, enabling true simultaneous multichannel lambda scanning on up to four channels. Multichannel lambda mode facilitates both live and post-processing spectral unmixing for excellent spectral separation results. With up to four dynamic ranges, bright and dim signals can be optimally separated by independently adjusting the sensitivity of each detector.

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Multichannel TruSpectral Detection with Sixteen-Channel Unmixing

TruSpectral detection works independently on all microscope channels, enabling true simultaneous multichannel lambda scanning on up to four channels. Multichannel lambda mode facilitates both live and post-processing spectral unmixing for excellent spectral separation results. With up to four dynamic ranges, bright and dim signals can be optimally separated by independently adjusting the sensitivity of each detector.

Spectral Unmixing

The FV3000 system’s spectral deconvolution algorithm enables overlapping spectra to be separated based on the spectral information from lambda stack images. Fluorescence cross-talk between channels can be eliminated by the unmixing algorithm during both live image acquisition and post-processing, enabling clean separation of up to sixteen fluorophores.

Spectral Unmixing

Spectral unmixing of a PtK2 cell labeled with YOYO-1, Alexa Fluor 488, Rhodamine-phalloidin, and MitoTracker Red using multi-channel lambda stack image.

. , Platelets bound to a thrombosis in the blood vessel of a mouse. Images taken at 30 fps in full frame by a resonant scanner with 2 CH GaAsP PMTs. Image data courtesy of: Dr. Takuya Hiratsuka, Dr. Michiyuki Matsuda, Graduate School of Biostudies, Kyoto University.	Two Scanner Options Choose between two scan units: a traditional galvanometer scanner (FV3000) or a hybrid galvanometer/resonant scanner (FV3000RS). Using the galvanometer scanner and Olympus super resolution technology (FV-OSR), obtain resolutions down to 120 nm with a high signal-to-noise ratio Galvanometer unit offers flexible scanning options, including precise tornado scanning, as well as multipoint stimulation with 100 ms switching time Hybrid scan unit features both a galvanometer scanner for high-precision scanning as well as a resonant scanner for high-speed imaging Resonant scanner enables you to capture 30 frames per second with a full field of view at 512 × 512 pixels, or up to 438 frames per second by clipping down in Y to capture critical live physiological events, such as calcium ion flux No Compromise Between Speed and Field of View Many high-speed scanning methods restrict the field of view, limiting your ability to examine large areas with multiple cells. The resonant scanner in the FV3000RS microscope maintains a full 1X field of view with FN 18, even at a video rate of 30 frames per second. Clipping the Y axis enables speeds up to 438 frames per second.

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Platelets bound to a thrombosis in the blood vessel of a mouse. Images taken at 30 fps in full frame by a resonant scanner with 2 CH GaAsP PMTs.
Image data courtesy of: Dr. Takuya Hiratsuka, Dr. Michiyuki Matsuda, Graduate School of Biostudies, Kyoto University.

Two Scanner Options

Choose between two scan units: a traditional galvanometer scanner (FV3000) or a hybrid galvanometer/resonant scanner (FV3000RS).

Using the galvanometer scanner and Olympus super resolution technology (FV-OSR), obtain resolutions down to 120 nm with a high signal-to-noise ratio
Galvanometer unit offers flexible scanning options, including precise tornado scanning, as well as multipoint stimulation with 100 ms switching time
Hybrid scan unit features both a galvanometer scanner for high-precision scanning as well as a resonant scanner for high-speed imaging
Resonant scanner enables you to capture 30 frames per second with a full field of view at 512 × 512 pixels, or up to 438 frames per second by clipping down in Y to capture critical live physiological events, such as calcium ion flux

No Compromise Between Speed and Field of View

Many high-speed scanning methods restrict the field of view, limiting your ability to examine large areas with multiple cells. The resonant scanner in the FV3000RS microscope maintains a full 1X field of view with FN 18, even at a video rate of 30 frames per second. Clipping the Y axis enables speeds up to 438 frames per second.

Rolling Average Processing While high-speed scanning with low laser power minimizes phototoxicity, it often decreases the signal-to-noise ratio, making it difficult to obtain high-resolution time-lapse images. With rolling average processing, you can adjust high-speed time-lapse images to achieve better signal-to-noise ratios while maintaining the time scale and keeping the original data.	(Left) Raw 30 fps data acquired at low laser power (0.05%, 488 nm). (Right) Rolling average processing (10 frames) on 30 fps data acquired at low laser power.

Rolling Average Processing

While high-speed scanning with low laser power minimizes phototoxicity, it often decreases the signal-to-noise ratio, making it difficult to obtain high-resolution time-lapse images. With rolling average processing, you can adjust high-speed time-lapse images to achieve better signal-to-noise ratios while maintaining the time scale and keeping the original data.

Rolling average processing

(Left) Raw 30 fps data acquired at low laser power (0.05%, 488 nm).
(Right) Rolling average processing (10 frames) on 30 fps data acquired at low laser power.

	Macro-to-Micro Observation See data in context with macro-to-micro observation. With the FV3000 system’s redesigned light path, generate detailed overview images with magnifications as low as 1.25X, then easily identify structures to image at higher magnification. Mosaic imaging enables you to acquire continuous 3D (XYZ) and 4D (XYZT) images of adjacent fields of view. The entire process from image acquisition to stitching can be fully automated, saving time and generating more meaningful data.

Macro-to-Micro Observation

See data in context with macro-to-micro observation. With the FV3000 system’s redesigned light path, generate detailed overview images with magnifications as low as 1.25X, then easily identify structures to image at higher magnification. Mosaic imaging enables you to acquire continuous 3D (XYZ) and 4D (XYZT) images of adjacent fields of view. The entire process from image acquisition to stitching can be fully automated, saving time and generating more meaningful data.

TruSight Deconvolution: Image Processing for Higher Resolution Remove blur and obtain clearer, sharper images with TruSight deconvolution. Specialized cellSens algorithms for the FV3000 confocal microscope enable a seamless workflow from acquisition to publication with the click of a button. Take advantage of GPU processing for even faster results.	Left: Raw confocal image / Right: Image with TruSight . ,

TruSight Deconvolution: Image Processing for Higher Resolution

Remove blur and obtain clearer, sharper images with TruSight deconvolution. Specialized cellSens algorithms for the FV3000 confocal microscope enable a seamless workflow from acquisition to publication with the click of a button. Take advantage of GPU processing for even faster results.

Left: Raw confocal image / Right: Image with TruSight

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. ,	Olympus Super Resolution (FV-OSR) Technology with Up to Four Simultaneous Channels Suitable for colocalization analysis, the Olympus Super Resolution imaging module can acquire four fluorescent signals either sequentially or simultaneously with a resolution of approximately 120 nm, nearly doubling the resolution of typical confocal microscopy. Requires no special fluorophores and works with a wide range of samples Easy to use with minimal training Add FV-OSR to any FV3000 confocal microscope system to achieve super resolution microscopy Learn more about the FV3000 Super Resolution Software Module

0.5 AU Confocal Image Deconvolved with cellSens Advanced Deconvolution

Olympus Super Resolution Plus cellSens Advanced Deconvolution. Note clear separation of punctate stains with OSR.

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Olympus Super Resolution (FV-OSR) Technology with Up to Four Simultaneous Channels

Suitable for colocalization analysis, the Olympus Super Resolution imaging module can acquire four fluorescent signals either sequentially or simultaneously with a resolution of approximately 120 nm, nearly doubling the resolution of typical confocal microscopy.

Requires no special fluorophores and works with a wide range of samples
Easy to use with minimal training
Add FV-OSR to any FV3000 confocal microscope system to achieve super resolution microscopy

Learn more about the FV3000 Super Resolution Software Module

TruFocus Time-Lapse Imaging TruFocus technology makes multi-position experiments and long-term time-lapse imaging more robust and reliable. TruFocus technology uses a minimally phototoxic infrared laser (Class 1) to identify the location of the sample plane and offers two modes for maintaining focus. Single shot autofocus (AF) mode allows you to set several focus positions, enabling efficient Z-stack acquisitions in multi-position experiments Continuous AF mode operates continually to keep the desired plane of observation precisely in focus, making it ideal for time-lapse measurements by avoiding focus drift due to temperature changes or added reagents TruFocus is compatible with a wide range of confocal imaging conditions, including dry objectives, plastic vessels, and silicone objectives	Related Videos

TruFocus Time-Lapse Imaging

TruFocus technology makes multi-position experiments and long-term time-lapse imaging more robust and reliable. TruFocus technology uses a minimally phototoxic infrared laser (Class 1) to identify the location of the sample plane and offers two modes for maintaining focus.

Single shot autofocus (AF) mode allows you to set several focus positions, enabling efficient Z-stack acquisitions in multi-position experiments
Continuous AF mode operates continually to keep the desired plane of observation precisely in focus, making it ideal for time-lapse measurements by avoiding focus drift due to temperature changes or added reagents
TruFocus is compatible with a wide range of confocal imaging conditions, including dry objectives, plastic vessels, and silicone objectives

Conventional objectives (left) versus X Line objectives (right)	High-Performance Microscopic Imaging with X Line Objectives Providing broad chromatic aberration correction, uniform images, and a high numerical aperture, X Line objectives enhance the FV3000 confocal microscope’s image quality. Learn more

Conventional objectives (left) versus X Line objectives (right)

High-Performance Microscopic Imaging with X Line Objectives

Providing broad chromatic aberration correction, uniform images, and a high numerical aperture, X Line objectives enhance the FV3000 confocal microscope’s image quality.

Learn more

Deep Tissue Observation with Silicone Immersion Objectives

Olympus offers four high-NA silicone immersion objectives that deliver excellent performance for live cell imaging.

The refractive index of silicone oil (ne≈1.40) is close to that of living tissue (ne≈1.38), enabling high-resolution observations deep inside living tissue with minimal spherical aberration
Silicone oil does not dry out or harden, so you don’t need to refill oil during extended time-lapse observations

. ,	Enhance the Reliability of Colocalization Analysis with a Low Chromatic Aberration Objective (PLAPON60XOSC2) This oil immersion objective minimizes lateral and axial chromatic aberration in the 405–650 nm spectrum, enabling you to acquire reliable colocalization images and measure objects with positional accuracy. The objective also compensates for chromatic aberration through near infrared (up to 850 nm), making it ideal for quantitative microscopy imaging. Magnification: 60X NA: 1.4 (oil immersion) WD: 0.12 mm Chromatic aberration compensation range: 405–650 nm Optical data provided for each objective

Performance Comparison of PLAPON60XOSC2 and UPLXAPO60XO

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Enhance the Reliability of Colocalization Analysis with a Low Chromatic Aberration Objective (PLAPON60XOSC2)

This oil immersion objective minimizes lateral and axial chromatic aberration in the 405–650 nm spectrum, enabling you to acquire reliable colocalization images and measure objects with positional accuracy. The objective also compensates for chromatic aberration through near infrared (up to 850 nm), making it ideal for quantitative microscopy imaging.

Magnification: 60X
NA: 1.4 (oil immersion)
WD: 0.12 mm
Chromatic aberration compensation range: 405–650 nm
Optical data provided for each objective

Optimized Configurations for Electrophysiological Experiments

Synchronize confocal imaging events with electrophysiology equipment via the trigger signal I/O interface box. The I/O interface box also converts voltage signals into images that can be treated in the same manner as fluorescence images. This enables you to capture voltage-based images in synchrony with photostimulation by the confocal scanner.

Get more details about the IO Interface Box

Software

Intuitive Software The FV3000 software streamlines your entire confocal imaging workflow, from acquisition to analysis. Customizable and savable layouts make it easy to tailor the interface to your workflow and experiment needs, no matter the level of complexity.

Intuitive Software

The FV3000 software streamlines your entire confocal imaging workflow, from acquisition to analysis. Customizable and savable layouts make it easy to tailor the interface to your workflow and experiment needs, no matter the level of complexity.

Intuitive Software

Live 3D Rendering and Analysis

See your data unfold in real time with the FV3000 software’s live 3D image display function. 3D images can be constructed during image acquisition and shown as live images.

Additional 3D cell analysis is available using optional NoviSight software. Take advantage of the software’s powerful capabilities to quantify spheroid cells or other 3D objects in microplate-based experiments.

Learn more

Live 3D Rendering

Fucci induced spheroid of the HT29 cell line
Image data courtesy of: Yuji Mishima, Ph.D., Kiyohiko Hatake M.D., Ph.D. Clinical Chemotherapy, Cancer Chemotherapy Center, Japanese Foundation for Cancer Research.

3D cell analysis

Various 3D cell analyses are available with optional NoviSight software.

Multi-Area Time-Lapse and Microplate Imaging The Multi-Area Time-Lapse (MATL) module provides robust and accurate time-lapse data through precise control over motorized stage movements, enabling you to generate detailed overview microscope images to see data in context. Pair the MATL module with the well navigator module to achieve more functionality with sophisticated, intuitive controls for a wide range of cell culture vessels and custom microplates.

Multi-Area Time-Lapse and Microplate Imaging

The Multi-Area Time-Lapse (MATL) module provides robust and accurate time-lapse data through precise control over motorized stage movements, enabling you to generate detailed overview microscope images to see data in context. Pair the MATL module with the well navigator module to achieve more functionality with sophisticated, intuitive controls for a wide range of cell culture vessels and custom microplates.

Multi-Area Time-Lapse and Microplate Imaging

Reduce Complexity with the Sequence Manager

The Sequence Manager software module handles complex protocols with ease. Multiday time-lapse experiments are controlled with microsecond scan accuracy and millisecond sequence execution accuracy. Various complex protocols can be performed, including:

Switching between high and low magnification
Variable-interval time-lapse experiments
Photostimulation between imaging by FRAP or FRET (acceptor photobleaching)

Reduce Complexity with the Sequence Manager

Quantitative Microscopy Image Analysis

The FV3000 confocal microscope incorporates a suite of optional analysis functions to complete your imaging workflow and provide quantitative data.

Count and Measure: calculate the number, size, luminosity, and morphology of cell segments
Colocalization: analyze overlapping fluorescent spectra

Count and Measure

Count and Measure
Sample courtesy of: Hidetaka Kosako, Fujii Memorial Institute of Medical Sciences, Tokushima University

Colocalization

FRET and FRAP Experiments

The FV3000 microscope paired with the cellSens Life Science Analysis module enables easy acquisition and analysis of FRET and FRAP experiments.

FRAP: estimate τ/2 and the mobile/immobile fraction by fitting the curve of luminosity change caused by fluorescence recovery after bleaching
FRET: measure FRET efficiency by acceptor photobleaching, ratio imaging, and sensitized emission

Example of FRET analysis (acceptor photobleaching)

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Example of FRET analysis (acceptor photobleaching)

Example of FRAP analysis

Ratio Imaging and Intensity Modulated Display (IMD)

The FV3000 microscope's ratio imaging analysis software includes an Intensity Modulated Display (IMD) function that displays quantitative fluorescence ratio changes during both standard and high-speed acquisitions. This function is particularly useful for calcium and FRET imaging where a pure ratio display provides poor contrast in background areas.

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tsGFP1-mito reveals heterogeneity in mitochondrial thermogenesis in HeLa cells.
The images of the ratio (ex 405 nm/ex 488 nm) in tsGFP1-mito-expressing cells before and after CCCP treatment at 37 °C (98.6 °F). Scale bars indicate 10 μm (whole image) and 3 μm (inset).
Image data courtesy of Shigeki Kiyonaka Ph.D., Yasuo Mori Ph.D., Molecular Biology Field, Department of Synthetic Chemistry and Biological Chemistry, Kyoto University.

(Left) Raw CFP/YFP ratio, (Right) IMD of CFP/YFP ratio

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Cardiomyoctye
Image data courtesy of Yusuke Niino and Atsushi Miyawaki, Cell Function Dynamics, Brain Science Institute of RIKEN.

. , Bioluminescence of RA-induced differentiating cells at day 12 from Bmal1:luc stably transfected ES cells Image data courtesy of: Kazuhiro Yagita, M.D. Ph.D. Department of Physiology and Systems Bioscience, Kyoto Prefectural University of Medicine Reference: Proc Natl Acad Sci U S A. 107(8): 3846–3851 (2010)	Object Tracking Automatically detect, track, and analyze moving objects in time-lapse images using the cellSens Object Tracking module. The tracking function provides a powerful and intuitive tool to quantify dynamic processes, such as cell movement and division.

Time-Dependent Change in the Intensity of Cells

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Bioluminescence of RA-induced differentiating cells at day 12 from Bmal1:luc stably transfected ES cells
Image data courtesy of: Kazuhiro Yagita, M.D. Ph.D. Department of Physiology and Systems Bioscience, Kyoto Prefectural University of Medicine
Reference: Proc Natl Acad Sci U S A. 107(8): 3846–3851 (2010)

Object Tracking

Automatically detect, track, and analyze moving objects in time-lapse images using the cellSens Object Tracking module. The tracking function provides a powerful and intuitive tool to quantify dynamic processes, such as cell movement and division.

Remote Development Kit (RDK) Module The Remote Development Kit enables remote control and programming of certain FV3000 microscope functions in languages like Python, C++, and Matlab. The RDK module is a powerful tool for users with programming experience to unlock even greater potential behind the system.

Remote Development Kit (RDK) Module

The Remote Development Kit enables remote control and programming of certain FV3000 microscope functions in languages like Python, C++, and Matlab. The RDK module is a powerful tool for users with programming experience to unlock even greater potential behind the system.

Configurations

Choose the Configuration that Suits Your Application

Inverted microscope

Suitable for observing cells cultured in a vessel
TruFocus unit enables time-lapse observations with consistent focus
Add a stage-top or full enclosure incubator to maintain the environmental conditions of cultured cells

Upright microscope (configured for slide imaging)

Optimized for fixed tissue and glass slide specimens
Motorized 7-position nosepiece and condenser enable automated transitions from low to high magnification for macro-to-micro applications

Upright microscope (configured for electrophysiology)

Ample space around the objectives enables easy installation of patch-clamp devices
Add extra space by lowering the stage position for experiments that require large sample handling
Swing and slider nosepieces are available so objectives can be easily changed without interfering with the patch-clamp setup

Specifications

		FV3000	FV3000RS
Main Laser Combiner	Visible Light Laser	405 nm: 50 mW, 488 nm: 20 mW, 561 nm: 20 mW, 640 nm: 40 mW One optional laser port for the sub laser combiner or optional laser unit
Optional Laser	Sub Laser Combiner	Maximum 3 laser units as follows: 445 nm: 75 mW, 514 nm: 40 mW, 594 nm: 20 mW, fiber connected to main laser combiner
	Single Laser Unit	445 nm: 75 mW, 514 nm: 40 mW, or 594 nm: 20 mW, directly connected to main laser combiner
	Single NIR Laser Unit	730 nm: 30 mW, 785 nm: 100 mW, connected to scanner via optional port
Scanner	Scanning Method	2 silver-coated galvanometer scanning mirrors	2 silver-coated galvanometer scanning mirrors 1 silver-coated resonant and 1 silver-coated galvanometer scanning mirrors
	Galvanometer Scanner (Normal Imaging)	Scanning Resolution: 64 × 64 to 4096 × 4096 pixels Scanning Speed (One Way): 512 × 512 with 1.1 s–264 s. pixel time: 2 µs–1000 µs Scanning Speed (Round Trip): 512 × 512 with 63 ms–250 ms, 256 × 256 with 16 ms–125 ms Optical Zoom: 1X–50X in 0.01X increments Scan Rotation: Free rotation (360 degree) in steps of 0.1 degree Scanning Mode: PT, XT, XZ, XY, XZT, XYT, XYZ, XYλ, XYZT, XYλT, XYλZ, XYλZT ROI scanning, rectangle clip, ellipse, polygon, free area, line, free line and point, tornado mode only for stimulation
	Resonant Scanner (High-Speed Imaging)	-	Scanning Resolution: 512 × 32 to 512 × 512 pixels Scanning Speed: 30 fps at 512 × 512, 438 fps at 512 x 32 Optical Zoom: 1X–8X in 0.01X increments Scanning Mode: XT, XZ, XY, XZT, XYT, XYZ, XYλ, XYZT, XYλT, XYZ, XYλZT ROI scanning, rectangle clip, line
	Pinhole	Single motorized pinhole, pinhole diameter ø50–800 µm (1 µm steps)
	Field Number (FN)	18
	Dichromatic Mirror Turret	8 positions (high-performance DMs and 10/90 mirror)
	Optional Unit for Scanner	Laser power monitor, optional laser port
Spectral Detector	Detector Module	Cooled GaAsP photomultiplier (high-sensitivity type) or Multi-Alkali photomultiplier, 2 channels
	Spectral Method	Motorized volume phase holographic transmission diffraction grating, motorized adjustable slit, selectable wavelength bandwidth: 1–100 nm, wavelength resolution: 2 nm
	Dichromatic Mirror Turret	8 positions (high-performance DMs and mirror)
NIR Detector	Detector Module	GaAs photomultiplier tube, 1 channel or 2 channels with filter cube
Fluorescence Illumination Unit		External fluorescence light source, fiber adaptor to the optical port of the scan unit, motorized switching between the LSM light path and fluorescence illumination
Transmitted Light Detector Unit		Module with integrated external transmitted light photomultiplier detector and LED lamp, motorized switching

Microscope

	Inverted frame	Upright frame (for imaging)	Upright frame (for electrophysiology)
Microscope Frame	Motorized inverted microscope IX83 (IX83P2ZF)	Motorized fixed stage upright microscope BX63L	Motorized fixed stage upright microscope BX63L
Revolving Nosepiece	Motorized sextuple revolving nosepiece	Motorized septuple revolving nosepiece	Coded swing nosepiece Coded slider nosepiece
Condenser	Motorized long working distance condenser	Motorized universal condenser	Manual long working distance condenser
Focus Stroke	Built-in motorized nosepiece focus Stroke: minimum increment: 0.01 μm

Software

Basic Features	GUI designed for darkroom environment. User-arrangeable layout. Acquisition parameter reload features. Hard disk recording capability; adjust laser power and HV with Z-stack acquisition. Z-stack with alpha blending, maximum-intensity projection, iso-surface rendering.
2D Image Display	Each image display: single-channel side-by-side, merge, cropping, live tiling, live tile, series (Z/T/λ), LUT: individual color setting, pseudo-color, comment: graphic and text input.
3D Visualization and Observation	Interactive volume rendering: volume rendering display, projection display, animation displayed. 3D animation (maximum intensity projection method, α blending) 3D and 2D sequential operation function.
Image Format	OIR image format 8/16-bit gray scale/index color, 24/ 32/ 48-bit color, JPEG/ BMP/ TIFF image functions, Olympus multi-tif format.
Spectral Unmixing	Fluorescence spectral unmixing modes (up to 16 channels)
Image Analysis	Region and line measurements, intensity plot over time/Z, colocalization analysis.
Statistical Processing	2D data histogram display.
Optional Software	Motorized-stage control Mapping and multipoint simulation Sequence manager Virtual channel acquisition Microplate navigation Remote development kit Super resolution imaging (FV-OSR) Digital camera control function Deconvolution FRET and FRAP analysis Automatic object measurement and classification Object tracking TruAI deep-learning technology NoviSight 3D cell analysis Automated macro-to-micro imaging

FV3000

Overview

Next Generation FLUOVIEW for the Next Revolutions in Science

High-Sensitivity Image Multiplexing from Violet to NIR

Macro-to-Micro Imaging and Super Resolution Microscopy

Hybrid Scanning for High-Speed Imaging and Increased Productivity

Related Videos

Related Videos

Accurate Time-Lapse Imaging

Deep Tissue Observation with Silicone Objectives

Automated Organoid Imaging

Applied Technologies

TruSpectral Detection

How TruSpectral Detection Technology Works

Sensitivity and Accuracy

Enhanced Quantum Efficiency

Multichannel TruSpectral Detection with Sixteen-Channel Unmixing

Spectral Unmixing

Two Scanner Options

No Compromise Between Speed and Field of View

Rolling Average Processing

Macro-to-Micro Observation

TruSight Deconvolution: Image Processing for Higher Resolution

Olympus Super Resolution (FV-OSR) Technology with Up to Four Simultaneous Channels

TruFocus Time-Lapse Imaging

Related Videos

High-Performance Microscopic Imaging with X Line Objectives

Deep Tissue Observation with Silicone Immersion Objectives

Related Videos

Enhance the Reliability of Colocalization Analysis with a Low Chromatic Aberration Objective (PLAPON60XOSC2)

Optimized Configurations for Electrophysiological Experiments

Software

Intuitive Software

Live 3D Rendering and Analysis

Multi-Area Time-Lapse and Microplate Imaging

Reduce Complexity with the Sequence Manager

Quantitative Microscopy Image Analysis

FRET and FRAP Experiments

Ratio Imaging and Intensity Modulated Display (IMD)

Object Tracking

Remote Development Kit (RDK) Module

Configurations

Choose the Configuration that Suits Your Application

Inverted microscope

Upright microscope (configured for slide imaging)

Upright microscope (configured for electrophysiology)

Components

Software

Scanners

Specifications

Microscope

Software

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