Olympus Logo

Confocal Laser Scanning Imaging
X Line High-Performance Objectives and the FV3000 Microscope

Efficient Experiments

Cover a magnification range from 40X to 100X without changing objectives using a 40X X Line objective with a 1.4 numerical aperture and the FV3000 microscope's optical zoom.

Learn more about the FV3000

Efficient Experiments
Comparison of the edge

(60× oil immersion objectives)

No Compromise in Confocal Image Quality

The 60X X Line oil immersion objective's improved numerical aperture, image flatness, and chromatic aberration correction deliver outstanding images and reliable data in a wide wavelength range (400–1000 nm).

Superior Image Quality at 405 nm Excitation

X Line objectives provide uniform, high-quality images at the 405 nm excitation wavelength over a full field of view (FN18) for precise quantitative analysis, such as counting cell nuclei stained by DAPI.

Comparison of 60x objective
placholder image
Conventional objectives (left) versus X Line objectives (right)

Brain section of Fucci2 transgenic mouse
Stitched image of 12X12 images acquired by FV3000 using 60X oil immersion objective (NA1.42)
Cyan: DAPI (405nm)  Magenta: mCherry ‘561nm)
Image Courtesy of:  Laboratory for Cell Function Dynamics, RIKEN Center for Brain Science
Takako Kogure, Atsushi Miyawaki

Multicolor Panoramic Image

Improved image flatness enables you to acquire superior stitched images with less zooming and in a wide wavelength range starting at 400 nm.

Exceptional Color Accuracy

With chromatic aberration correction from 400 to 1000 nm, X Line objectives deliver accurate multicolor images and quantitative results during colocalization analysis.

Image #1: Comparison of chromatic aberration measured by FLUOVIEW FV3000 using TetraSpeck TM Microsphere.
Cyan: 405nm excitation, Magenta: 640nm excitation 
Image #2: HeLa cell labeled by FISH technique  CEP17(Spectrum Green), CEP18(Spectrum Orange), Nuclear (DAPI)
When observing with conventional objectives, signals located at the bottom of cell nuclear appears outside the nuclear. Scal bar: 2 μm
Axial chromatic aberration
placholder image
Left: Conventional objectives / Right: X Line

Related Products

FV3000

FV3000

  • Available in galvanometer-only (FV3000) or hybrid galvanometer/resonant (FV3000RS) scanner configurations 
  • Highly efficient and accurate TruSpectral detection on all channels 
  • Optimized for live cell imaging with high sensitivity and low phototoxicity 

Learn More

*Banner Image: Fixed brain coronal slice (thickness 1mm) of Thy1-YFP-H mouse  Staining: DAPI by ChemScale Mounting solution: SCALEVIEW-SMt (ND=1.49) 
                          MIP image acquired by FV3000 using UPLXAPO60XO(NA1.42)  Cyan: DAPI(405nm), Yellow: YFP(488nm)
 By courtesy of: Laboratory for Cell Function Dynamics RIKEN Center for Brain Science  Tetsushi Hoshida, Hiroshi Hama, Atsushi Miyawaki

Sorry, this page is not
available in your country.

Sorry, this page is not
available in your country.

This site uses cookies to enhance performance, analyze traffic, and for ads measurement purposes. If you do not change your web settings, cookies will continue to be used on this website. To learn more about how we use cookies on this website, and how you can restrict our use of cookies, please review our Cookie Policy.

OK