Overview

	Flexibility Meets Quality Performance The Alpha³ by PhaseView was developed to offer researchers a flexible, high-performing light sheet solution. As an advanced fluorescence microscope, the Alpha³ offers smart optical focus sweeping for uniform, artifact-free imaging across the entire field of view. The system can be easily upgraded with options such as fast scanning (Smart 3D Scanning), XY tiling, and temperature controls to meet various experimental needs. The QtSPIM software provides a clear and intuitive interface for collecting X, Y, Z, θ, T, λ images at maximum speed. Get in Touch

Flexibility Meets Quality Performance

The Alpha³ by PhaseView was developed to offer researchers a flexible, high-performing light sheet solution. As an advanced fluorescence microscope, the Alpha³ offers smart optical focus sweeping for uniform, artifact-free imaging across the entire field of view. The system can be easily upgraded with options such as fast scanning (Smart 3D Scanning), XY tiling, and temperature controls to meet various experimental needs. The QtSPIM software provides a clear and intuitive interface for collecting X, Y, Z, θ, T, λ images at maximum speed.

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4 Key Values of the Alpha³ System

Dual illumination system with real-time optical focus sweeping produces even illumination across the entire field of view.
Corrosion-resistant sample holders for imaging flexibility from in vivo live imaging of cell cultures to whole cleared tissues.
Optional Smart 3D Scanning technology for perturbation-free image acquisition at 75 images/second.
Compatible with a full selection of Olympus objectives for macro to micro imaging of a variety of specimens.

Fixed Stentor, GFP cilia and DAPI poly-nuclei at 10x magnification

Fixed drosophila egg, 3 channel imaging at 20x

Specifications

Please visit PhaseView's website for the specifications.

Learn more about the Alpha³ specifications

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Applied Technologies

Dual Illumination

Through the integration of dual illumination units, the multi-directional light sheet provides artifact-free images of absorbing or scattering specimens. When paired with the optical focus sweeping module, the Alpha³ provides optimized imaging sharpness across the entire field of view, delivering unprecedented image quality.

Mouse embryo

Real-time Optical Focus Sweeping

Optical focus sweeping alleviates spatial and temporal resolution constraints for 3D image acquisition. This optional module maintains the focus of the light sheet across the entire field of view for optimal image clarity. In other words, the exposed field is always in the thinnest region of the light sheet. This principle relies on a tunable lens for focal plane sweeping that is synchronized with the rolling shutter of the camera.

Real-time focus sweeping for optimized image clarity over the entire field of view.

Cleared mouse brain image taken without (left) and with sharp optical sectioning (right).

Smart 3D Scanning

Instead of moving the sample to perform Z-plane scanning, the specimen is mounted in a fixed position while the illumination and the detection planes move simultaneously through the sample. As the sample remains in a steady position, vibrations and perturbation issues are alleviated. The illumination plane is scanned using a galvanometric mirror, while the detection plane scanning is achieved using a remote focusing device to provide high-speed perturbation-free 3D imaging at 75 images/second.

Using an electrically driven tunable lens and galvo mirror, the Alpha3 system provides fast and vibration-free imaging.

The sample chambers and holders are resistant to corrosive media and clearing solutions. Available in vivo chamber with temperature and CO2 control for long-term time-lapse imaging.	Versatile Design A variety of sample holders and mounting accessories provide the flexibility to image anything from small specimens up to whole organs. For in vivo imaging, precise temperature and CO2 controls are also available.

The sample chambers and holders are resistant to corrosive media and clearing solutions.

Available in vivo chamber with temperature and CO2 control for long-term time-lapse imaging.

Versatile Design

A variety of sample holders and mounting accessories provide the flexibility to image anything from small specimens up to whole organs. For in vivo imaging, precise temperature and CO2 controls are also available.