Evident LogoOlympus Logo

Inverted Microscopes

Inverted microscopes are valuable pieces of equipment for laboratory cell analysis. Using advanced imaging techniques, inverted microscopes enable you to observe cells for life science research through the use of fluorescence and brightfield observation.

Evident offers a broad selection of routine inverted microscopes, inverted phase contrast microscopes, inverted fluorescence microscopes, and confocal and super resolution inverted microscopes. Designed with ergonomics and precise imaging solutions, our inverted microscopes offer a range of powerful tools to advance your research.

Get in Touch 

Inverted Microscopes

Customizable Spinning-Disk Confocal Systems

CrestOptics Confocal Series

CrestOptics solutions are flexible and powerful spinning-disk confocal systems for live-cell imaging. Combined with Evident’s range of microscopes and optics and 89 North’s laser diode illuminators, these customizable systems give researchers a powerful platform to expand their live-cell imaging capabilities.

  • CICERO: Improve your widefield microscope imaging capabilities
  • X-Light V3: Simultaneous dual camera imaging for challenging live-cell applications
  • Flexible laser lines, including NIR, catered to your research needs
Digital Imaging System


  • Easy-to-use, all-in-one microscope system
  • Publication-quality images in a few clicks
  • Fast, efficient data management
Compound Microscope System

IXplore Standard

Optimized for basic multicolor fluorescence imaging and routine experiments, the IXplore Standard system is easy to operate and ergonomically designed. Even with standard cell culture vessels, it captures high-quality, publication-worthy images while providing accurate and repeatable results at high magnifications. The IXplore Standard system’s simplified workflow and ease of use facilitate a wide range of standard imaging applications.

  • High repeatability and accuracy for standard imaging tasks
  • Benefit from the same optical capabilities found in high-end IXplore systems
  • Easily upgrade to encoded functionality to boost the reproducibility of experiments
Learn More
Automated Microscope System

IXplore Pro

The IXplore Pro automated system acquires panoramic, multichannel images without a complex workflow. Easing experiment setup, the Graphical Experimental Manager offers fully automated multidimensional observation capabilities. The microscope’s high-precision ultrasonic stage, automated focusing, and bright, uniform fluorescence illumination facilitate image stitching applications.  

  • Automated multidimensional observation (X, Y, Z, T, wavelength, and positions)
  • Boost your statistics with multiwell plate screening
  • Acquire fluorescence panoramic images of large samples, such as brain slices
  • Create 3D optical sections and enhance resolution with TruSight™ deconvolution
Learn More
Live Cell Imaging Microscope System

IXplore Live

Designed to reduce photobleaching and phototoxicity, the IXplore Live system is optimized for physiological experiments involving live cell and tissue observation. Offering precise environmental control and enhanced rigidity, it supports long-term cell viability and stability for time-lapse imaging applications, such as in cancer, stem cell, and brain research.

  • Maintain focus accurately and reliably in time-lapse experiments with TruFocus™ Z-drift compensation system
  • Discover the real morphology of your cells with Olympus silicone immersion optics
  • Real-time controller helps limit cell disturbance, enabling physiologically relevant data
Learn More
TIRF Imaging Microscope System

IXplore TIRF

For membrane dynamics, single-molecule detection, and colocalization experiments, the IXplore TIRF system enables sensitive simultaneous multicolor TIRF (total internal reflection fluorescence) imaging for up to four colors. Olympus’ cellTIRF system provides stable motorized individual laser-angle control, providing equal evanescent wave penetration for high-contrast, low noise images. Our TIRF objectives feature high SNR, high NA, and correction collars to adjust for cover glass thicknesses and temperature.

  • Exact colocalization of up to four markers thanks to individual penetration depth control
  • Take advantage of Olympus’ TIRF objective with the world's highest NA of 1.7*
  • Intuitive setup of complex experiments with the Graphical Experiment Manager (GEM), cellFRAP, and U-RTCE
* As of July 25, 2017. According to Olympus research.
Learn More
Confocal Imaging Microscope System

IXplore Spin

The IXplore Spin system features a spinning disk confocal unit that enables fast 3D image acquisition, a large field of view, and prolonged cell viability in time-lapse experiments. Researchers can use it to perform rapid 3D confocal imaging with high resolution and contrast at greater depths for imaging into thicker samples. The spinning disk also helps to cut down on photobleaching and phototoxicity of samples upon excitation.

  • Real-time controller (U-RTCE) helps optimize the device’s speed and precision during automated acquisition
  • TruFocus™ Z-drift compensation system maintains focus for each frame
  • Precise 3D imaging with improved light collection using X Line™ objectives
  • Upgrade to the IXplore SpinSR super resolution system as your research progresses
Learn More
Super Resolution Microscope System

IXplore SpinSR

The IXplore SpinSR system is our confocal super resolution microscope optimized for 3D imaging of live cell specimens. Like the IXplore Spin system, it has a spinning disk system for fast 3D imaging while limiting phototoxicity and bleaching. However, it achieves super resolution images down to 120 nm XY and enables you to switch between widefield, confocal, and super resolution with the click of a button.

  • Sharp, clear super resolution imaging down to 120 nm XY, owing to Olympus Super Resolution (OSR)
  • Prolonged cell viability in confocal time-lapse imaging due to less phototoxicity and bleaching
  • Use two cameras simultaneously to achieve fast, two-color super-resolution imaging
  • Super resolution imaging with the world’s first plan apochromat objectives with a numerical aperture (NA) of 1.5*
* As of November 2018. According to Olympus research.
Learn More
Compact Cell Culture Microscope


The CKX53 microscope eases the cell and tissue culture workflow, simplifying steps such as live cell observation, cell sampling and handling, image capture, and fluorescence observation. Its integrated phase contrast system, compact, ergonomic design, and stable performance enable simple, efficient cell observation. The universal sample holder and expandable stage accommodate a wide variety of cell culture container types and sizes.

  • Precentered phase contrast
  • Inversion contrast (IVC) technique provides clear three-dimensional views
  • Fluorescence with a 3-position slider
  • View multilayer tissue flasks up to 190 mm (7.5 in.) in height up thanks to the detachable condenser

Learn More

 was successfully added to your bookmarks

View Bookmarks   Close

Maximum Compare Limit of 5 Items

Please adjust your selection to be no more than 5 items to compare at once

Inverted Research Microscope FAQs

How does an inverted microscope work?

An inverted microscope is similar to a compound microscope, but the components are positioned in an inverted configuration—thus the name “inverted” microscope. With a compound microscope, the transmitted light source and condenser lens are beneath the stage and specimen. In an inverted microscope, the condenser lens and transmitted light source are placed above the specimen while the objective, fluorescence light source, and mirror turret are positioned below.

The component positioning in an inverted microscope means that transmitted light is directed from above and the resulting image is then viewed from below. This makes inverted microscopes suitable for viewing cell culture vessels, including glass flasks or petri dishes where the specimens adhere to the bottom of the vessel.

How to use an inverted microscope?

Inverted microscopes use high-magnification optics for precise cell viewing and analysis using both transmitted and reflected light paths. Many inverted microscope configurations have a fixed stage and rely on a high-magnification objective lens that can be moved along a vertical axis to adjust the focus of a specimen (allow the specimen to be brought closer or moved farther away from the lens). Once focused, the user can observe the specimen through the oculars on an open-frame microscope, or from a computer screen if using an inverted microscope with a camera. Explore these videos to learn how to add oil to the objective of an inverted microscope and how to load a sample slide on the stage.

What is an inverted fluorescence microscope?

As previously stated, an inverted microscope is a microscope with its transmitted light source and condenser above the stage pointing down, while the objectives and turret are below the stage pointing up. Open-frame inverted microscopes, like our IXplore™ Standard and IXplore Pro systems, are great solutions for labs looking to adapt and customize a system to their needs. The systems work with multimodal solutions, such as TIRF illuminators or spinning disk confocal modules, to offer flexible imaging options. When using open-frame inverted microscopes for fluorescence imaging, a dedicated darkroom is usually required to avoid interfering signals from room lights.

If there is not enough space for a dedicated darkroom, an enclosed, all-in-one inverted fluorescence microscope, such as the APEXVIEW™ APX100 digital imaging system, is the best option. An all-in-one inverted fluorescence microscope is an automated research microscope with a box frame that enables users to image under normal lighting conditions. Its automated workflow removes complex steps typically involved in setting up an advanced fluorescence or confocal system, and these compact and versatile inverted microscopes can be placed almost anywhere in a lab or core facility.

Inverted Microscope Resource Videos

IXplore Solutions-Based Microscopy

Each system in the IXplore inverted microscope series is tailored to fit a specific research application to help scientists efficiently accomplish their goals.

Loading a Sample on an Inverted Microscope

This video will show you how to load a sample onto your IX83 inverted microscope using a standard 1 by 3-inch slide.

Silicone Oil Immersion Objectives for Live Cell Imaging

When looking at live cells, the right objectives will enable you to see clearly. This video looks at the ways in which silicone oil immersion objectives achieve precise and higher resolution images during cell and tissue observation.

Sorry, this page is not
available in your country.

Need assistance?

Get in Touch

Sorry, this page is not available in your country