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Overview

	Modular High-Content Screening Station for Life Sciences The scanR modular microscope-based imaging platform provides fully automated image acquisition and data analysis of biological samples through deep-learning technology. Get in Touch

Modular High-Content Screening Station for Life Sciences

The scanR modular microscope-based imaging platform provides fully automated image acquisition and data analysis of biological samples through deep-learning technology.

Get in Touch

Flexible, Modular Hardware

The scanR screening station combines the modularity and flexibility of a microscope-based setup with the automation, speed, and throughput demanded by high-content screening. Well-suited for standard assays and assay development, the modular design makes the scanR station adaptable to R&D lab applications or multiuser environments.

Spinning Disc Confocal System

Spinning Disk Confocal System

Compatible with the Olympus IXplore SpinSR super resolution microscope system incorporating the Yokogawa CSU-W1 scanner unit
Micro-lens-based disks and laser excitation provide seamless confocal image quality at high speed

Robot Loading System

Use with a plate-loading robot system for automated high-throughput screening

Incubation System

Add any IX83 compatible incubation system for stringent control of temperature, humidity, and CO2 levels

TIRF and FRAP System (with cellSens Software)

Use with the Olympus IXplore family and cellSens software to perform advanced imaging experiments such as TIRF and FRAP

Related Videos	Comprehensive Software The system’s flexible design enables it to meet the requirements for quantitative imaging and image analysis in modern cell biology, molecular biology, systems biology, and medical research. Fully automated image acquisition and data analysis of biological samples Designed for multiwell plates, slides, and custom-built arrays Powerful analysis module for biological functional assays Ideal for assay development and high-content screening Accommodates fixed and live cells

Comprehensive Software

The system’s flexible design enables it to meet the requirements for quantitative imaging and image analysis in modern cell biology, molecular biology, systems biology, and medical research.

Fully automated image acquisition and data analysis of biological samples
Designed for multiwell plates, slides, and custom-built arrays
Powerful analysis module for biological functional assays
Ideal for assay development and high-content screening
Accommodates fixed and live cells

Meets the Needs of Many Assays

The scanR system excels in drug discovery applications, including showing the biochemical effects of compounds on the cellular level and drug-induced changes at gene expression levels. The solution can measure apoptosis, micronuclei, or DNA fragmentation (comet assays) and covers a wide range of screening applications:

Cell counting
Gene expression
Intracellular transport
Translocation
Cell proliferation
Promyelocytic leukemia (PML) body assays
Bacterial and viral infection assays
Cell-cycle analysis
Cell-array screens
Protein localization and colocalization
Live cell assays including kinetic analysis and gating on resulting response curves
Multicolor assays
Rare event analysis
Automated FISH analysis
Fluorescence analysis in tissue sections
Cell migration

Genome-wide screen on cell arrays to identify novel genes involved in intracellular transport machinery. Image courtesy of Dr R. Peperkok, EMBL, Heidelberg, Germany

Genome-wide screen on cell arrays to identify novel genes involved in intracellular transport machinery. Image courtesy of Dr R. Peperkok, EMBL, Heidelberg, Germany.

Chlamydia trachomatis infection assay. Image courtesy of Dr S. Hess, Max Planck Institute (MPI) for Infection Biology, Berlin, Germany.

Segmentation of binucleated cells and micronuclei counting. Image courtesy of Department for In-Vitro Toxicology, Fraunhofer Institute Toxicology and Experimental Medicine (ITEM), Hannover, Germany

Segmentation of binucleated cells and micronuclei counting. Image courtesy of Department for In-Vitro Toxicology, Fraunhofer Institute Toxicology and Experimental Medicine (ITEM), Hanover, Germany.

Flexible Module Options

The scanR solution not only satisfies the specific speed, endurance, and reliability requirements of a fully automated high-content screening system but also provides unmatched flexibility and adaptability with extensive expansion capabilities. This enables the scanR system to meet the specifications of a wider range of applications and budgets. Add modules with the following capabilities to your system:

Self-learning microscopy based on deep-learning technology
Measuring kinetic parameters
High-speed 3D deconvolution
Infrared (IR) laser hardware autofocusing (based on the IX83 ZDC)
And more

Contact application specialists to customize your system for your applications

High Speed Deconvolution

High-Speed TruSight Deconvolution

Comparison of wide field, 2D deconvolution, and 3D deconvolution detecting the fine structures of samples without compromising on throughput.

TruFocus with Infrared (IR) Laser Hardware Autofocus

The enhanced continuous AF mode keeps the desired plane of observation precisely in focus, even when adding reagents or during changes in room temperature.

Self-learning Microscopy based on Deep Learning Technology

Self-Learning Microscopy with Deep-Learning Technology

From left to right: AI prediction of nuclei positions (blue), green fluorescent protein (GFP) histone 2B labels showing nuclei (green), and raw brightfield transmission image (gray).

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Applied Technologies

Gating and Classification The powerful data-analysis concepts successfully applied in cytometry are adapted to suit the demands of analyzing large-image datasets. Multidimensional image data are displayed in two-dimensional scatter plots or one-dimensional histograms; clustered data populations of interest can be selected using graphical tools. Gates from different plots can be combined with Boolean operators to create complex classification schemes.	A hierarchical gating approach enables intuitive selection of populations, which may also be visualized in galleries.

Gating and Classification

The powerful data-analysis concepts successfully applied in cytometry are adapted to suit the demands of analyzing large-image datasets.
Multidimensional image data are displayed in two-dimensional scatter plots or one-dimensional histograms; clustered data populations of interest can be selected using graphical tools.
Gates from different plots can be combined with Boolean operators to create complex classification schemes.

A hierarchical gating approach enables intuitive selection of populations, which may also be visualized in galleries.

Get Started Quickly

The included pre-trained neural network models enable you to start using the AI fast. Using the pre-trained models, you can begin detecting nuclei and cells in most standard conditions. Even confluent cells and dense nuclei can be reliably distinguished.

Control and validation measures are built in to help ensure the accuracy and robustness of the AI analysis results.

Accurate object segmentation: raw data (left), standard threshold segmentation (middle), TruAI instance segmentation (right). Instance segmentation reliably separates difficult-to-distinguish objects that are very close together, such as cells or nuclei in colonies or tissue.

Image screenshot detail following data acquisition by scanR demonstrating the detection and separation of labels. Courtesy of Dr. R. Pepperkok, EMBL Heidelberg, Germany.	Object Detection and Analysis Powerful object detection modules segment nuclei, cells, or other structures. Select multiple detection algorithms and adapt them to the objects of interest. Based on the segmentation results, features to be extracted can be selected from a list of over 100 object parameters. Facilitates a wide range of cell-based assays.

Image screenshot detail following data acquisition by scanR demonstrating the detection and separation of labels. Courtesy of Dr. R. Pepperkok, EMBL Heidelberg, Germany.

Object Detection and Analysis

Powerful object detection modules segment nuclei, cells, or other structures.
Select multiple detection algorithms and adapt them to the objects of interest.
Based on the segmentation results, features to be extracted can be selected from a list of over 100 object parameters.
Facilitates a wide range of cell-based assays.

Immediate Quality Control Images and objects are reciprocally linked to their related data points: Clicking on a data point loads the relevant image in the display window and highlights the object in question. Clicking on an object in the image display window highlights the related data points in the scatter plots and histograms. Create a gallery view of all images of a selected or gated data population to enable direct, visual comparison of larger image sets with relevant information.	Results are visualized in heatmaps or exported to tables. Easily display an overview of full wells.

Immediate Quality Control

Images and objects are reciprocally linked to their related data points:

Clicking on a data point loads the relevant image in the display window and highlights the object in question.
Clicking on an object in the image display window highlights the related data points in the scatter plots and histograms.

Create a gallery view of all images of a selected or gated data population to enable direct, visual comparison of larger image sets with relevant information.

Results are visualized in heatmaps or exported to tables. Displaying an overview of full wells is simple.

Results are visualized in heatmaps or exported to tables. Easily display an overview of full wells.

Related Videos	Multilevel Acquisition After an initial prescan, the scanR analysis software can identify all the potential objects of interest. In an automated workflow, the analysis results are used to selectively scan the objects of interest in a second, targeted screen.

Multilevel Acquisition

After an initial prescan, the scanR analysis software can identify all the potential objects of interest. In an automated workflow, the analysis results are used to selectively scan the objects of interest in a second, targeted screen.

Measuring Kinetic Parameters with the Kinetic Module Classify live cells, nuclei, and other objects by their time-variant properties. Evaluate tracking curves based on values (mean static parameters, such as intensity, area, ratio, shape factor, etc.) measured over time. Evaluate and analyze static parameters, such as intensity or the ratio of fluorescence markers, position, size, or shape over time. Curves are condensed into single characteristic values (kinetic parameters). Plot the kinetic parameters in 1D or 2D histograms, and gate populations based on their specific time-variant properties.	hES cells expressing FUCC (CA) biosensor. Courtesy of Dr. Silvia Santos, The Francis Crick Institute, London, UK.

Measuring Kinetic Parameters with the Kinetic Module

Classify live cells, nuclei, and other objects by their time-variant properties.
Evaluate tracking curves based on values (mean static parameters, such as intensity, area, ratio, shape factor, etc.) measured over time.
Evaluate and analyze static parameters, such as intensity or the ratio of fluorescence markers, position, size, or shape over time.
Curves are condensed into single characteristic values (kinetic parameters).
Plot the kinetic parameters in 1D or 2D histograms, and gate populations based on their specific time-variant properties.

hES cells expressing FUCC (CA) biosensor. Courtesy of Dr. Silvia Santos, The Francis Crick Institute, London, UK.

	Combines High-End Imaging and High-Content Analysis Run Olympus cellSens live cell imaging software on the same system as the scanR solution so that you can use the same setup simultaneously for screening and high-end imaging. Obtain high-quality image detail for the most demanding screening applications using 2D and 3D constrained iterative deconvolution algorithms. The fast and easy-to-use algorithms remove out-of-focus blur and background to reveal essential structural details. Helpful for in-depth analysis requiring high-resolution structural details.

A Tale of Two Systems

Combines High-End Imaging and High-Content Analysis

Run Olympus cellSens live cell imaging software on the same system as the scanR solution so that you can use the same setup simultaneously for screening and high-end imaging.
Obtain high-quality image detail for the most demanding screening applications using 2D and 3D constrained iterative deconvolution algorithms.
The fast and easy-to-use algorithms remove out-of-focus blur and background to reveal essential structural details.
Helpful for in-depth analysis requiring high-resolution structural details.

Self-Learning Microscopy

Self-learning microscopy opens new horizons in high-content analysis. Applications range from previously impossible image segmentation and classification tasks to quantitative analysis of extremely low signal levels, simplifying staining protocols, label-free analysis, and more.

Self-Learning Microscopy

Example workflow using self-learning microscopy to generate an AI model for label-free analysis of challenging brightfield images. The cell nuclei of Hela cells are GFP-labeled for the training phase to show the system how to analyze the brightfield images.

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A New Way of Thinking

Example application: Robust segmentation of cell nuclei at different signal levels, enabling a dramatic reduction of light exposure for quantitative analysis.

The user has full control of the training experiment design.

Many challenging analysis conditions can be covered during the training phase.

The learned AI analysis protocol can be validated in depth and with ease with the software’s unique data exploration and analysis interface.

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Specifications

Microscope Frame	Olympus IX83 inverted microscope, one or two decks Motorized stage, Märzhäuser SCAN IM 120 × 80 for the Olympus IX83 microscope
Fluorescence LED Options	Lumencor SpectraX: 6 independent LED channels CoolLED pE-300ultra: 3 independent LED channels Application-optimized bandpass filters Lifetime > 20,000 hours
Transmitted-Light Illumination Options	Transmitted-light illumination for visual inspection only (no transmitted-light screening) Transmitted-light illumination for screening and visual inspection including fast shutter (transmitted-light screening supported) Optional DIC (differential interference contrast) or phase contrast
Hardware Control and System Synchronization (optional)	Real-time controller with additional CPU, independent of the OS of the imaging PC Temporal resolution: 1 ms Timing precision: < 0.01 ms Hardware-synchronized multitask acquisition (illumination control, emission filters, shutters, etc.) Precise camera control via external trigger
Camera Options	Hamamatsu ORCA-Flash 4.0 V3, high-sensitivity cooled sCMOS camera with large 18.8 mm (0.74 in.) sensor chip Hamamatsu ORCA-Flash 4.0 LT, an economic sCMOS camera with large 18.8 mm (0.74 in.) sensor chip Hamamatsu ORCA-Fusion, sCMOS camera with large 21.2 mm (0.83 in) sensor chip Hamamatsu ORCA-Fusion BT, ultra-low noise sCMOS camera with large 21.2 mm (0.83 in.) sensor chip
Objective Options (supports Olympus X Line objectives)	Objectives for “thin” (0.1 mm–0.2 mm [0.004 in.–0.008 in.]) substrates, cover slips, and glass bottom plates (2X, 4X, 10X, 20X, 40X, 60X, 100X) Objectives for “thick” (~1 mm [0.04 in.]) substrates, plastic bottom plates, and slides (2X, 4X, 10X, 20X, 40X, 60X, 100X) Phase contrast objectives for “thin” (0.1 mm–0.2 mm [0.004 in.–0.008 in.]) substrates, coverslips, and glass bottom plates (10X, 20X, 40X) Phase contrast objectives for “thick” (~1 mm [0.04 in.]) substrates, coverslips, and glass bottom plates (10X, 20X, 40X)
Filter Sets	Single-band filter sets (specifications as requested) Multiband filter sets (specifications as requested)
scanR System Software	2 independent software modules: scanR acquisition software and scanR analysis software Shading correction workflow to compensate for shading and optimize spatial intensity homogeneity during and post-acquisition The software modules can be installed on the same or on different workstations (Windows 10 64-bit)
scanR Acquisition Software	Workflow-oriented configuration and user interface Powerful software autofocus procedures that can be combined with an optional IR laser hardware autofocus function, 2-step coarse and fine autofocus, object-based autofocus, or image-based autofocus Plate manager with predefined formats (slides, multiwell plates) and editing interface to create and edit customized formats (spotted arrays) Shading correction to compensate for shading and optimize spatial intensity homogeneity Time-lapse screening, Z-stack screening, multicolor screening (unlimited number of acquisition channels) Support for integration into automated sample-preparation lines, ex., scriptable interfaces for liquid handling
scanR Analysis Software	Automated image and data analysis for standard assays and assay development Online and offline multicore analysis Image processing, image analysis, particle detection, and parameter extraction and calculation Cytometric data exploration, analysis, gating, and classification Powerful and flexible gating concept including automated population detection Direct link between data points, objects, and images Assays-based work flow and advanced scientific assay development functionality
Computer	Imaging computer (latest generation PC) , Windows 10 64 bit
Additional Options	scanR AI deep-learning solution Time-lapse kinetic analysis module—a unique cytometric approach to better analyze and understand live-cell dynamics 3D deconvolution module (64-bit operating system required) Confocal option with Yokogawa CSU-W1 with one or two cameras Two-camera simultaneous acquisition IR laser hardware autofocus function based on the ZDC of the Olympus IX83 microscope series Plate-loading robot Encoded magnification changer IX3-CAS Fast-emission filter wheel for high-speed imaging in “Sedat” configuration Additional scanR analysis workstation Second license for scanR analysis software scanR Analysis Viewer
Two Systems in One Setup	Can be combined with cellSens live cell imaging software for full versatility of a high-end imaging system