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Overview

	Transforming Precision Imaging Empower Your Confocal Microscopy Imaging Experiments Transform your images with the new FLUOVIEW™ FV4000 confocal laser scanning microscope. Advanced imaging technology enables higher precision images to empower researchers with more reliable data from their samples. With our breakthrough SilVIR™ detector at the core of the system, achieve much lower noise, higher sensitivity, and improved photon resolving capabilities. With the FV4000 confocal microscope, researchers can acquire higher-quality, quantitative image data in less time and with less effort. Get in Touch

Transforming Precision Imaging

Empower Your Confocal Microscopy Imaging Experiments

Transform your images with the new FLUOVIEW™ FV4000 confocal laser scanning microscope. Advanced imaging technology enables higher precision images to empower researchers with more reliable data from their samples. With our breakthrough SilVIR™ detector at the core of the system, achieve much lower noise, higher sensitivity, and improved photon resolving capabilities. With the FV4000 confocal microscope, researchers can acquire higher-quality, quantitative image data in less time and with less effort.

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Experience the systems innovations, including:

Game-changing dynamic range for imaging from the macro scale to subcellular structures
Multiplex up to six channels simultaneously with TruSpectral™ technology
Redesigned high-speed, high-resolution scanners for fixed and live cell imaging
Improved depth and photosensitivity with pioneering near-infrared (NIR) capabilities and renowned optics
Peace of mind with the reliable, repeatable SilVIR detector
Industry-leading* ten laser lines with a broader spectral range from 405 nm to 785 nm
Modular design to meet researchers’ ever-changing needs, including the ability to upgrade to multiphoton imaging on a single system

*As of October 2023.

Easy-to-Acquire, Quantitative Confocal Data

The FV4000 confocal microscope uses our advanced, silicon-based SilVIR™ detector that makes it easier than ever to acquire precise, reproducible data.

SilVIR Next-Generation Detector Technology

The SilVIR detector combines two advanced technologies—a silicon photomultiplier (SiPM) and our patented* fast signal processing design.

High dynamic range
Low noise
No degradation of sensitivity
Less sensitivity variation among other detectors

*Patent number US11237047

Learn more about the SilVIR detector

40x Brain Stitch

Neurofilament-heavy chain (NFH) in green, myelin basic protein (MBP) in red, glutathione S-transferase pi 1 (GSTpi) in blue. Mouse cerebellum captured with a UPLXAPO40X objective.
Sample courtesy of Katherine Given, Ph.D. Principal Investigator, Neurobiology University of Colorado Anschutz Medical Campus, Aurora, Colorado

Conventional detector

SiLVIR detector

The histogram on the image captured using the SilVIR detector shows a discrete pattern where the intensity can be converted to the photon number. The detector’s fluorescence intensity can be quantified as the photon number, and the background level is extremely low.

More Information from Your Confocal Images

The system’s updated TruSpectral technology combined with high sensitivity SilVIR detectors enable you to see more by making it possible to multiplex up to six channels simultaneously.

Stictch A01 GOO1 Projection Z

The neurovascular unit of a mouse hippocampus. Blue; DAPI cell nuclei, Green; GFAP AF488. Astrocytes, yellow; DsRed pericytes, magenta; collagen IV AF647 basement membrane of blood vessels, gray; AQ-4. Astrocytes water channel.
Sample courtesy of: Hiroshi Hama and Atsushi Miyawaki, Cell Function Dynamics, RIKEN CBS.

CytoSkelton 4 color cell

Cytoskeleton sample: HeLa cells stained with DAPI (blue), Pericentrin (Centrosome, green), a-Tubulin (Microtubules, Alexa-568; red) and phalloidin (Actin, Alexa-647; magenta).
Sample Courtesy of: Sample preparation Alexia Ferrand; sample acquisition Sara R. Roig and Alexia Ferrand. Imaging Core Facility, Biozentrum, University of Basel.

Flexible Macro to Micro Imaging

The macro-to-micro workflow enables you to easily observe the target sample from the macro level—whole body or tissue—down to the cell or subcellular level.

Overview image of a Drosophila wing

Edge image of a Drosophila wing

Overview and edge images of a Drosophila wing (42-hour pupation). Stained with phalloidin (AlexaFluor 405, F-actin, Cyan), anti-phosphotyrosine antibody (AlexaFluor 555, cell surface, red), and anti-HRP antibody (AlexaFluor 647, axon, blue). Sample courtesy of: Sun Zhengkuan, Shigeo Hayashi, Laboratory for Morphogenetic Signaling, RIKEN Center for Biosystems Dynamics Research, Japan.

Gentler High-Speed Time-Lapse Confocal Imaging

HeLa cells labeled by MitoView 720. XYZT imaging by 1K resonant scanner for 30 min.

Time-lapse imaging is easier with smart features:

Capture every moment of live cell dynamics: resonant scanner can acquire high-resolution images over a wide area
Minimal phototoxicity: the scanner’s short pixel dwell time reduces the time the focused laser beam rests on a single spot
Better signal-to-noise ratio: the SilVIR detector’s high sensitivity produces higher quality images at higher speeds
Greater precision: rolling average processing maintains qualifications and time resolution

Reproducible Image Data Between Users and Systems

The SilVIR detector has less sensitivity loss over time compared to previous-generation detector technologies. With our laser power monitor (LPM) and TruFocus™ Z-drift compensator, achieve reproducible images under consistent conditions for better reproducibility. Different users on different days can acquire the same precise images using the same settings. Even the images acquired by different FV4000 microscopes can be compared and discussed using the same photon number intensity scale.

Microscope Support and Service You Can Count On

We designed the FV4000 system to be easy to maintain:

Semiconductor-based SilVIR detector is stable and durable
Laser power monitor continuously checks the illumination condition and makes adjustments to maintain consistent laser power
System admin can view log files to keep track of the service maintenance schedule

We stand behind our products with a commitment to fast service and technical support. We offer various support plans to keep your microscope running at peak performance at a predictable cost as well as remote support options, so you don’t need to wait for an engineer or specialist to visit if you’re having an issue.

Microscope Support and Service You Can Count On

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Applied Technologies

See Further with NIR-Enabled Confocal Microscopy

The system’s enhanced technologies provided expanded multiplexing to see more in one image.

NIR imaging offers greater multiplexing capabilities by extending the excitation (λ_Ex) and detection (λ_Em) spectral profile of the FV4000 system. This enables additional dyes to be used to help minimize emission signal overlapping.

Multiplex up to six channels with updated TruSpectral™ technology and our high sensitivity SilVIR™ detectors
High-efficiency volume phase hologram (VPH) grating and slit can detect an industry-leading 400 nm to 900 nm wavelength range* with a minimum 1 nm step
Expand your fluorochrome choices with up to six channels of broadband or red-shifted detectors to minimize damage and reduce autofluorescence
Modular laser combiners allow up to 10 laser lines from 405 nm to 785 nm in parallel

*As of March 2023.

Cell nuclei (DAPI; blue)

cell membrane (AF488; green)

nuclear pore (AF561; yellow)

microtubule (Qdot605; magenta)

mitochondria (MitoTracker DeepRed; cyan)

actin (AF750 phalloidin; gray)

HeLa cells labeled by 6 fluorochromes.

HeLa cells labeled by 6 fluorochromes.
Cell nuclei (DAPI; blue), cell membrane (AF488; green), nuclear pore (AF561; yellow),
microtubule (Qdot605; magenta), mitochondria (MitoTracker DeepRed; cyan), actin (AF750 phalloidin; gray).

High-Quality Optics for Efficient NIR Fluorescence Imaging

The FV4000 system's optical elements have a high transmission from 400 nm to 1300 nm, including the galvanometer and resonant scanner, which are coated in silver rather than aluminum.

Our award-winning X Line™ objectives are corrected for chromatic aberrations between 400–1000 nm. They also have a higher numerical aperture, excellent flatness, and very high transmittance from UV to NIR, increasing the multiplexing capabilities.

For improved colocalization reliability, our specialized A Line™ (PLAPON60XOSC2) oil immersion objective (ne~1.40) significantly minimizes chromatic aberration for strict colocalization analysis.

X Series

. , A total of 77 four-channel XYZ positions (11 × 7) were acquired using a 1K resonant scanner within 16 minutes to create the stitched image, which used to require 2 hours using a galvanometer scanner. The coronal section of an H-line mouse brain, cyan; DAPI (cell nuclei), green; YFP (neuron), yellow; Cy3 astrocytes, magenta; AlexaFluor 750 (microtubule). Sample courtesy of: Takako Kogure and Atsushi Miyawaki, Cell Function Dynamics, RIKEN CBS.	High-Quality Confocal Images at High Speed A unique combination of advanced technologies delivers high-quality images faster than conventional laser scanning microscope systems. High-resolution images at high speed: 1K × 1K resonant scanner at FN20 with 0.033 µs per pixel and the SilVIR detector enable you to rapidly acquire images with minimal noise Exceptional quality macro images: quickly acquire stitched macro images with outstanding quality to maximize your time and research potential

A total of 77 four-channel XYZ positions (11 × 7) were acquired using a 1K resonant scanner within 16 minutes to create the stitched image, which used to require 2 hours using a galvanometer scanner. The coronal section of an H-line mouse brain, cyan; DAPI (cell nuclei), green; YFP (neuron), yellow; Cy3 astrocytes, magenta; AlexaFluor 750 (microtubule). Sample courtesy of: Takako Kogure and Atsushi Miyawaki, Cell Function Dynamics, RIKEN CBS.

High-Quality Confocal Images at High Speed

A unique combination of advanced technologies delivers high-quality images faster than conventional laser scanning microscope systems.

High-resolution images at high speed: 1K × 1K resonant scanner at FN20 with 0.033 µs per pixel and the SilVIR detector enable you to rapidly acquire images with minimal noise
Exceptional quality macro images: quickly acquire stitched macro images with outstanding quality to maximize your time and research potential

Simple, Precise Super Resolution Imaging

Capture super resolution images using the FV4000 microscope with no dedicated hardware.

Easily observe subcellular structures using our A Line HR objectives and our super resolution software (FV-OSR)
FV-OSR automatically optimizes the confocal aperture to detect high-frequency components and enhance their contrast to achieve 120 nm resolution
Achieve super resolution images 8x faster than previous-generation systems thanks to the SilVIR detector and on-the-fly processing

Confocal mode 1AU (left) versus super resolution mode (right)

High-Resolution 3D Images in Thick Samples

HeLa cell spheroid labeled by DAPI (cyan, cell nuclei) and AlexaFluor790 (magenta, Ki-67). Imaging of the spheroid’s whole volume was possible by NIR 785 nm, although only surface area cell nuclei observation was possible using a 405 nm laser.

When imaging thicker samples, the FV4000 microscope enables you to capture high-resolution, 3D images.

Take advantage of NIR’s longer wavelength to penetrate deeper into tissue thanks to the SilVIR detector’s wide dynamic range and sensitivity
Image deeper with less scattering and absorption by taking advantage of the fact that light-scattering compounds—like melanin and heme—absorb less light between 700–1500 nm
Image significantly deeper than what’s possible with visible lasers thanks to 685 nm, 730 nm, and 785 diode lasers on the FV4000
High NA silicone objectives minimize spherical aberration and silicone oil doesn’t dry out, both advantageous for time-lapse imaging
Improve overall image quality and Z resolution using TruSight™ deconvolution for stunning 3D images of thick samples
Enjoy a seamless workflow from acquisition to publication with specialized cellSens™ software algorithms

Precise Dynamics of Live Cells with Less Damage

Typically, using longer wavelengths for fluorescence excitation for shorter periods of time is better for overall sample health. Using less phototoxic light means you can image for longer periods, enabling you to obtain more consistent and reproducible data from live cell imaging experiments.

The FV4000 system not only provides gentle time-lapse imaging via the 685 nm, 730 nm, and 785 nm lasers, but it also features a dedicated TruFocus Red Z-drift compensator to maintain the focus position. This upgraded TruFocus Red unit supports a larger range of wavelengths and is compatible with a wide range of objectives, including our high-performing X Line™ and A Line™ series.

Time-lapse photo stimulation: the laser injury was performed on C2C12 cells. The green pseudocolor represents the application of an FM 1-43 bath. The image was acquired with a 2 μs galvo scanner and a UPLSAPO60XOHR objective. A 405 nm laser was used for photodamage and a 488 was used to image. Sample courtesy of: Daniel Bittel and Jyoti Jaiswal, Center for Genetic Medicine Research, Children’s National Research Institute.

Time-lapse image of HeLa cells stained with Hoechst33342 (nuclear, blue), MitoTracker Green (mitochondria, green), LysoTracker Red (Lysosome, yellow), SiR-Tubulin (tubulin, magenta), POR-SA-Halo (ER, cyan). Hoechst33342: Ex 405 nm/Em, MitoTracker Green: LysoTrakcer Red: SiR-Tubulin: POR-SA-Halo: Sample courtesy of: Masayasu Taki, Ph.D., Institute of Transformative Bio-Molecules (WPI-ITbM), Nagoya University, Japan, Yuichi Asada and Ryusei Aruga, Graduate School of Science, Nagoya University, Japan.

A 17-hour, time-lapse image of HeLa cells stained with MitoTracker Red (mitochondria, magenta), POR-SA-Halo (ER, cyan). MitoTracker Red: Ex 561nm/Em, POR-SA-Halo: Ex 730nm/Em, Sample Courtesy of: Masayasu Taki, Ph.D., Institute of Transformative Bio-Molecules (WPI-ITbM), Nagoya University, Japan, Yuichi Asada and Ryusei Aruga, Graduate School of Science, Nagoya University, Japan.

Clear Images at Depth

Use our silicone immersion objectives with the FV4000 microscope and achieve clear images of features and structures deep within your sample. Silicone oil has a refractive index close to that of live cells or tissue, greatly reducing the spherical aberration as compared to air, water, or other oils. With less aberration, you can achieve clearer images of your sample at depth. And silicone immersion oil does not dry out at 37 ℃ (98.6 °F), making it effective for long-term time-lapse imaging.

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AI Solutions for Confocal Microscopy

Take your confocal imaging to the next level and save time during data analysis. While the microscope’s signal-to-noise ratio is already very good, TruAI™ technology denoise can further reduce the noise for stunning, data-rich resonant images.

To speed up image analysis, you can pretrain an AI model so that the system can automatically segment your image data, greatly reducing the workload of this often time-consuming manual process. Then, TruAI technology further streamlines the analysis so that you can get your data quickly.

TruAI Noise Reduction

Improve your resonant scanner image quality by incorporating TruAI noise reduction. Although resonant scanner images are effective in capturing cellular dynamics at high speeds with low damage, this usually causes a compromise in the S/N ratio. TruAI noise reduction can improve these images without sacrificing time resolution using pre-trained neural networks based on the noise pattern of the SilVIR™ detectors. These pre-trained TruAI noise reduction algorithms can be used for on-the-fly processing as well as post processing.

Processed with TruAI noise reduction (right)

Brain sample: coronal section (50 μm) of a mouse brain stained with DAPI (nuclei, cyan), GFAP (astrocytes, green/488), MAP2 (microtubule-associated protein 2, neurons, and dendritic processes, cyan/647) and MBP (myelin basic protein, red/568). Sample courtesy of: Sample preparation Alexia Ferrand; sample acquisition Sara R. Roig and Alexia Ferrand. Imaging Core Facility, Biozentrum, University of Basel.

Processed with TruAI noise reduction (right)

HeLa cell mitochondria labeled by MitoView 720 acquired using a 1K resonant scanner. The maximum photon number was 3 photons.

TruAI Image Segmentation

Image analysis requires data extraction using segmentation techniques based on intensity value thresholds. However, this can be time-consuming and is affected by the sample conditions.

TruAI image segmentation using deep learning helps streamline image processing and minimize sample variables for more accurate image analysis. It enables you to segment very weak fluorescence images or tissues that are usually difficult to extract using the simple thresholding method.

TruAI detects the glomeruli features (right)

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Configurations

The FV4000 microscope is engineered to be modular, making it easy for you to configure the system based on your applications and budget. You can start with a standard FV4000 and easily upgrade to multiphoton imaging by adding the MPE module as your research changes.

One Platform for Your Research Needs

Multiphoton and single-photon combination imaging in one sample is also possible. The FV4000MPE microscope is capable of second and third harmonic generation imaging, so different researchers or users can make the most out of the system. If your research requires a custom setup, the microscope’s modularity and optional ports enable you to customize the system to add extra lasers, cameras, detectors, and more.

Upgrade to FV4000MPE

Choose the Configuration that Suits Your Application

Inverted Microscope Frame

Upright Microscope Frame for Documentation

BX ePhys

Upright Microscope Frame for Electrophysiology

BX GF

Gantry Microscope Frame

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Specifications

Scanner	Galvanometer scanner (normal imaging)	64 × 64 to 4096 × 4096 pixels, 1 μs/pixel–1000 μs/pixel
	Resonant scanner scanner (high-speed imaging)	512 × 512 pixels, 1024 × 1024 pixels
	Field number (FN)	20
Spectral confocal detector	Detector	SilVIR detector (cooled SiPM, broadband type/red-shifted type)
	Maximum channels	Six channels
	Spectral method	VPH, detectable wavelength range 400 nm–900 nm
Laser	VIS laser	405 nm, 445 nm, 488 nm, 514 nm, 561 nm, 594 nm, 640 nm
	NIR laser	685 nm, 730 nm, 785 nm
	Laser power monitor	Built in
Image	High dynamic range photon counting (1G cps, 16-bit)