Contador de células automatizado
The Cell Counter model R1 automated cell counter is designed to reduce workloads and to standardize the cell counting process. Its unique software algorithm recognizes small cells < 8μm including peripheral blood mononuclear cells (PBMC) and nerve cells differentiated and induced from iPS cells. It automatically recognizes and counts individual cells even in clustered cells, performs size- and shape-based sorting, and identifies live/dead cells. This portable instrument equipped with user-friendly touchscreen provides reliable result throughout the cell culture workflow and accelerates the cell culture process.
*For research use only.
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Since cells are properly identified regardless of whether they are single or clustered, live or dead, the number of cells can be accurately counted with almost the same accuracy as counting with the human eye.
The average size of the cells varies depending on cell type. The maximum and minimum cell size can be adjusted to correctly detect the desired cells.
Live Cell Sensitivity function enables optimization of the recognition rate for small cells < 8μm, which was previously difficult to achieve.
Each detection parameter can be modified to fit the sample conditions. Up to 300 specific protocols can be easily saved and reloaded, which helps to obtain quick counting results for routine procedures.
By setting the desired concentrations, the dilution calculator automatically calculates the volume of cell suspension needed, which makes for a quick transfer to the subsequent procedures.
The Cell Counter can quickly count and present the total number of cells in a sample without Tryptan blue. When an approximate proportion of live/dead cells in the sample is known, the number of live cells can be roughly estimated, allowing for a faster procedure.
Thanks to the newly equipped Auto Exposure Mode, variations in Trypan Blue concentration are automatically corrected, allowing for stable count results.
The Cell Counter automatically stores up to 1,000 counts. The data can then be exported as a CSV file. Also, the counted results and image data can be stored as a TIFF, an annotated TIFF, or a PDF report file. Moreover, these data can easily be transferred to other storage devices through the USB ports equipped with the Cell Counter.
The report files can be obtained easily, containing the protocol used, the cell count results, the corresponding raw/ analyzed cell images, and histograms. There is no need to write down detailed settings for each cell count. Also, on the review mode, various reports can be viewed all at once on the touchscreen, including the numbers of live/ dead cells, average cell size.
The Cell Counter focuses on cells quickly and automatically using liquid lens technology, similar to the way human eyes focus, and provides the accuracy and reliability of cell counting. The lens can autofocus in the Z-axis direction at the right position, producing more accurate counting results and increasing the accuracy and reliability of cell counting.
The handle on the top of the counter makes it easy to transport the Cell Counter, and allows it to fit any workplace setting. A storage space for the AC adapter is conveniently built into the back of the counter. An extension cord can be stored easily with no hassle.
By using disposable counting slides specifically designed for the Cell Counter, cells are easily placed through the loading slits on the slide. Since two sets of cell counting are performed on one slide, cost saving measures are achieved.
Designed with usability in mind, the Cell Counter comes as a standalone automated cell counter with a steady operation platform. The stability of the large touchscreen at the lower part of the Cell Counter makes it possible to easily and smoothly count cells, view data, and print the results.
| Cell Counting Time *1 | Less than 10 s (manual focusing) |
|---|---|
| Less than 15 s (auto focusing) | |
| Cell Concentration Range | 5 x 104 - 1 x 107 cells/mL |
| Cell Diameter Range | 3 - 60 μm (optimal: 8 - 30 μm) |
| Output Information *2 | Total / Live / Dead cell concentration |
| Total / Live / Dead cell number | |
| Viability | |
| Average cell size | |
| Image Resolution | 5 Mpixels, Color |
| Focusing Mode | Automatic / Manual focusing with liquid lens |
| Adjustable Parameters | Size, Roundness, Dilution factor, Noise reduction, Declustering |
| Image Type | TIFF, annotated TIFF |
| Report Format | |
| Count Data Storage | 1,000 counts |
| Protocol Storage | 300 protocols |
| Display | LCD touchscreen: 800 x 480, 7inch |
| USB Port | 3 ports |
| Thermal Printer | Built-in |
| Dimensions (W x D x H) | 195 mm x 237 mm x 272 mm |
| Weight | 2.1 kg (without the external power adaptor) |
| Input Power | AC 100-240V 50/60Hz 1.2A |
*1: Cell counting at less than 1 x 106 cells/ml concentration of HeLa or HL-60 cells.
*2: Live / Dead cell concentration, number and cell viability can be available with trypan blue mode.
| Material | Polystyrene |
|---|---|
| Dimensions (W x D x H) | 75 mm x 25 mm x 2.4 mm |
| Chamber Volume | 10 μL |
| Quantity Per Box | 100 counts (50 slides, 2 chambers per slide) |
Name： Description
| Protocol | Results |
|---|---|
|
Noise reduction: 6
Roundness: 60% Min. cell size (μm): 3 Max. cell size (μm): 60 Declustering level: Medium |
Total cell conc. (cells/ml): 1.50 x 106 Live cell conc. (cells/ml): 1.38 x 106 Dead cell conc. (cells/ml): 1.19 x 105 Viability (%): 92.0 Average cell size (μm): 15.9 |
| Protocol | Results |
|---|---|
|
Noise reduction: 7
Roundness: 60% Min. cell size (μm): 3 Max. cell size (μm): 60 Declustering level: None |
Total cell conc. (cells/ml): 1.03 x 106 Live cell conc. (cells/ml): 9.37 x 105 Dead cell conc. (cells/ml): 9.72 x 104 Viability (%): 90.6 Average cell size (μm): 19.6 |
| Protocol | Results |
|---|---|
|
Noise reduction: 5
Roundness: 60% Min. cell size (μm): 3 Max. cell size (μm): 60 Declustering level: Medium |
Total cell conc. (cells/ml): 1.09 x 106 Live cell conc. (cells/ml): 1.07 x 106 Dead cell conc. (cells/ml): 1.78 x 104 Viability (%): 98.4 Average cell size (μm): 17.5 |
| Protocol | Results |
|---|---|
|
Noise reduction: 5
Roundness: 60% Min. cell size (μm): 3 Max. cell size (μm): 60 Declustering level: High |
Total cell conc. (cells/ml): 7.51 x 105 Live cell conc. (cells/ml): 7.19 x 105 Dead cell conc. (cells/ml): 3.17 x 104 Viability (%): 95.8 Average cell size (μm): 10.7 |
| Protocol | Results |
|---|---|
|
Noise reduction: 5
Roundness: 60% Min. cell size (μm): 3 Max. cell size (μm): 60 Declustering level: Medium |
Total cell conc. (cells/ml): 1.31 x 106 Live cell conc. (cells/ml): 1.22 x 106 Dead cell conc. (cells/ml): 8.91 x 104 Viability (%): 93.2 Average cell size (μm): 12.1 |
| Protocol | Results |
|---|---|
|
Noise reduction: 5
Roundness: 60% Min. cell size (μm): 3 Max. cell size (μm): 60 Declustering level: Medium |
Total cell conc. (cells/ml): 1.04 x 106 Live cell conc. (cells/ml): 1.00 x 106 Dead cell conc. (cells/ml): 3.61 x 104 Viability (%): 96.5 Average cell size (μm): 13.4 |
| Protocol | Results |
|---|---|
|
Noise reduction: 5
Roundness: 60% Min. cell size (μm): 3 Max. cell size (μm): 60 Declustering level: Medium |
Total cell conc. (cells/ml): 1.86 x 106 Live cell conc. (cells/ml): 1.80 x 106 Dead cell conc. (cells/ml): 5.34 x 104 Viability (%): 97.1 Average cell size (μm): 14.3 |
| Protocol | Results |
|---|---|
|
Noise reduction: 5
Roundness: 60% Min. cell size (μm): 3 Max. cell size (μm): 60 Declustering level: Medium |
Total cell conc. (cells/ml): 1.84 x 106 Live cell conc. (cells/ml): 1.82 x 106 Dead cell conc. (cells/ml): 2.23 x 104 Viability (%): 98.8 Average cell size (μm): 11.3 |
| Protocol | Results |
|---|---|
|
Noise reduction: 5
Roundness: 60% Min. cell size (μm): 3 Max. cell size (μm): 60 Declustering level: Medium |
Total cell conc. (cells/ml): 4.14 x 105 Live cell conc. (cells/ml): 3.52 x 105 Dead cell conc. (cells/ml): 6.23 x 104 Viability (%): 84.9 Average cell size (μm): 19.5 |
| Protocol | Results |
|---|---|
|
Noise reduction: 5
Roundness: 60% Min. cell size (μm): 3 Max. cell size (μm): 60 Declustering level: Medium |
Total cell conc. (cells/ml): 2.08 x 105 Live cell conc. (cells/ml): 1.44 x 105 Dead cell conc. (cells/ml): 6.32 x 104 Viability (%): 69.6% Average cell size (μm): 12.8 |
| Protocol | Results |
|---|---|
|
Noise reduction: 5
Roundness: 60% Min. cell size (μm): 3 Max. cell size (μm): 60 Declustering level: Medium |
Total cell conc. (cells/ml): 1.34 x 106 Live cell conc. (cells/ml): 1.32 x 106 Dead cell conc. (cells/ml): 1.81 x 104 Viability (%): 98.7 Average cell size (μm): 12.2 |
| Protocol | Results |
|---|---|
|
Noise reduction: 5
Roundness: 60% Min. cell size (μm): 3 Max. cell size (μm): 60 Declustering level: Medium |
Total cell conc. (cells/ml): 8.98 x 105 Live cell conc. (cells/ml): 8.11 x 105 Dead cell conc. (cells/ml): 8.69 x 104 Viability (%): 90.3 Average cell size (μm): 17.6 |
| Protocol | Results |
|---|---|
|
Noise reduction: 5
Roundness: 60% Min. cell size (μm): 3 Max. cell size (μm): 60 Declustering level: Medium |
Total cell conc. (cells/ml): 1.54 x 106 Live cell conc. (cells/ml): 1.49 x 106 Dead cell conc. (cells/ml): 5.87 x 104 Viability (%): 96.2 Average cell size (μm): 14.9 |
| Protocol | Results |
|---|---|
|
Noise reduction: 5
Roundness: 60% Min. cell size (μm): 3 Max. cell size (μm): 60 Declustering level: Medium |
Total cell conc. (cells/ml): 1.57 x 106 Live cell conc. (cells/ml): 1.45 x 106 Dead cell conc. (cells/ml): 1.23 x 105 Viability (%): 92.2 Average cell size (μm): 14.9 |
| Protocol | Results |
|---|---|
|
Noise reduction: 5
Roundness: 60% Min. cell size (μm): 3 Max. cell size (μm): 60 Declustering level: Medium |
Total cell conc. (cells/ml): 1.13 x 106 Live cell conc. (cells/ml): 8.72 x 105 Dead cell conc. (cells/ml): 2.62 x 105 Viability (%): 76.9 Average cell size (μm): 16.5 |
| Protocol | Results |
|---|---|
|
Noise reduction: 5
Roundness: 60% Min. cell size (μm): 3 Max. cell size (μm): 60 Declustering level: Medium |
Total cell conc. (cells/ml): 4.74 x 105 Live cell conc. (cells/ml): 4.43 x 105 Dead cell conc. (cells/ml): 4.07 x 104 Viability (%): 91.4 Average cell size (μm): 16.2 |
| Protocol | Results |
|---|---|
|
Noise reduction: 5
Roundness: 60% Min. cell size (μm): 3 Max. cell size (μm): 60 Declustering level: Medium |
Total cell conc. (cells/ml): 1.52 x 106 Live cell conc. (cells/ml): 1.33 x 106 Dead cell conc. (cells/ml): 1.96 x 105 Viability (%): 87.1 Average cell size (μm): 14.1 |
| Protocol | Results |
|---|---|
|
Noise reduction: 5
Roundness: 60% Min. cell size (μm): 3 Max. cell size (μm): 60 Declustering level: High |
Total cell conc. (cells/ml): 1.23 x 106 Live cell conc. (cells/ml): 1.21 x 105 Dead cell conc. (cells/ml): 2.26 x 104 Viability (%): 98.2 Average cell size (μm): 15.3 |
| Protocol | Results |
|---|---|
|
Noise reduction: 5
Roundness: 60% Min. cell size (μm): 3 Max. cell size (μm): 60 Declustering level: Medium |
Total cell conc. (cells/ml): 1.36 x 106 Live cell conc. (cells/ml): 1.33 x 106 Dead cell conc. (cells/ml): 3.16 x 104 Viability (%): 97.7 Average cell size (μm): 17.2 |
| Protocol | Results |
|---|---|
|
Noise reduction: 5
Roundness: 0% Min. cell size (μm): 3 Max. cell size (μm): 60 Declustering level: Medium |
Total cell conc. (cells/ml): 1.78 x 106 Live cell conc. (cells/ml): 1.50 x 106 Dead cell conc. (cells/ml): 2.81 x 105 Viability (%): 84.2 Average cell size (μm): 13.1 |
| Protocol | Results |
|---|---|
|
Noise reduction: 5
Roundness: 60% Min. cell size (μm): 3 Max. cell size (μm): 60 Declustering level: Medium |
Total cell conc. (cells/ml): 7.66 x 105 Live cell conc. (cells/ml): 7.21 x 105 Dead cell conc. (cells/ml): 4.45 x 104 Viability (%): 94.2 Average cell size (μm): 16.0 |
| Protocol | Results |
|---|---|
|
Noise reduction: 5
Roundness: 40% Min. cell size (μm): 3 Max. cell size (μm): 60 Declustering level: Medium |
Total cell conc. (cells/ml): 1.22 x 106 Live cell conc. (cells/ml): 1.12 x 106 Dead cell conc. (cells/ml): 1.02 x 105 Viability (%): 91.6 Average cell size (μm): 13.4 |
Copyright OLYMPUS CORPORATION, All rights reserved.
Copyright OLYMPUS CORPORATION, All rights reserved.
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