Life Science Solutions

Fluorochrome Data Tables

The following tables contain data about various fluorescence excitation and emission properties of over 75 commonly used fluorochromes. They are intended to provide a reference source that relates the spectral properties of these fluorochromes to commercially available filtering cubes. Much of this data was retrieved from F. H. Kasten's excellent reference on fluorescence microscopy and probes. Table 1 is provided as a reference listing of the codes used in Table 2 for filter types.

Filter Color Codes
CodeFilter Type
UUltraviolet
VViolet
BVBlue-Violet
BBlue
IBInterference
Blue Filter
GGreen
IGImmuno Gold
Staining Method
BFBrightfield,
Phase Contrast,
and DIC only
Table 1

The table below (Table 2) contains data on the peak excitation and emission wavelengths of over 75 selected fluorochromes along with suggested applications and excitation filter cube type. The selection of a wideband, superwide band or narrow band filter cube will depend on the condition of the specimen and observation purpose.

Fluorescence Filter Cubes

Discover how variations in excitation and barrier filters allow certain wavelengths to pass.

In general, a wide band filter cube is suitable in most cases. For some specimens where more brightness is desired, a superwide filter cube may be applied at some expense to contrast. If more contrast for a specific wavelength band is required for a sufficiently bright specimen, a narrow band filter cube may be appropriate.

Fluorochrome Excitation/Emission/Cube/Application Data
FluorochromeExcitation WavelengthEmission WavelengthApplicable ExcitationComment
Fluorochrome
Antibody Method
 
Allophyco-
cyanin (APC)
650660IYAntibody labeling
7-Amino-4-Methyl-
coumarin-3-Acetic Acid (AMCA)
350450UAntibody Labeling
BODIPY FL503512B, IBAntibody Labeling
Cascade Blue376425UAntibody Labeling
Fluorescein-
Isothiocyanate (FITC)
490520IBAntibody Labeling
Phycoerythrin
B (PE-B)
545576GAntibody Labeling
Phycoerythrin
R (PE-R)
490, 565578G, IBAntibody Labeling
Rhodamine B-Iso-
thiocyanate (RITC)
570595GAntibody Labeling
Texas Red596620IYAntibody Labeling
Tetramethylrhodamine-
Isothyocyanate (TRITC)
541572IGAntibody Labeling
Protein Modification 
8-Anilino-1-Napthalene Sulfonic
Acid Mg-salt (ANS)
385485V, U, BVHydrophobic Part Probe
Coumarin Maleimide (CMP)385465UThiol Group
Cy 3552565IGAmino Group,
Antibody Labeling
Cy 5650667IYAmino Group,
Antibody Labeling
Dimethylaminonaphthalene-
5-Sulfonic Acid (DANS)
380520UThiol Group
Dansyl Chloride335500UAmine Labeling
Dansyl Hydrazine336531UCarbonyl Group
Eosin-Isothiocyanate524548IBAmine Labeling
Fluorescamine390460U, VAmine Labeling
Lucifer Yellow CH435530BV, BAldehyde, Ketone
Monochlorobimane380461UThiol Group
o-Phtalaldehyde (OPT)340455UAmine Labeling
Resorufin570585GThiol Group
SITS350420UAmine Labeling
Nuclei, Nucleic Acid 
Acridine Orange490530, 640B, IBSingle/Double-stranded
Nuclieic Acid
Acridine Yellow470550BNucleic Acid
Acriflavine-Feulgen430515BVDNA
Auramine O-Feulgen460550BDNA
7-Amino-
Actinomycin D
555655IY, IGG-C Range
Bis-Aminophenyl-
Oxabizole (BAQ)-Feulgen
380470UDNA
Chromomycin A3450570B, BVG-C Range
4, 6-Diamidino-2-
Phenylindole HCl (DAPI)
372456UA-T Range
Ethidium Bromide545605G, IGDNA•RNA
Hoechst 33258365465UA-T Range
Hoechst 33342355465UA-T Range
Mithramycin395570BVG-C Range
Olivomycin430545BVG-C Range
Pararosaniline-
Feulgen
560625GDNA
Propidium Iodide530615IB, G, IGDNA•RNA
Pyronin Y540570IGDNA•RNA
Quinacrine Mustard385525BVA-T Range, Q Band
Bone 
Acidic Fuchsin
(Pararosaniline)
540630GCalcification
Calcein495520IBBone Growth
Calcein Blue375435UBone Growth
Tetracycline390560BV, UCalcification
Xylenol Orange440, 570610GBone Growth
Carbonydrate Modification 
Acriflavine SO2, PAS430515BVSugar
Cell Viability Judgement & Living Cell Staining 
Carboxyfluoresceindiacetate
(CFDA)
495520IBEsterase Substrates
Fluoresceindiacetate (FDA)495520IBEsterase Substrates
Calcein/AM495520IBEsterase Substrates
BCECF/AM500530IBEsterase Substrates
Acridine Orange490590B, IBAcid Organanelle
Dead Cell Staining 
Ethidium Bromide545605G, IBCell membrane
Impermeability
Ethidium Homodimer528617G, IBCell membrane
Impermeability
Propidium Iodide530615G, IBCell membrane
Impermeability
Nerve Cell Tracers 
Evans Blue550610GRetrograde Labeling
Fast Blue360410URetrograde Labeling
Granular Blue375410URetrograde Labeling
Lucifer Yellow CH430535BVTracer
True Blue373404URetrograde Labeling
Membranes 
8-Anilino-Naphthalene
Sulfonic Acid (ANS)
385485VNegative Ionicity
DiI550565G, IGPositive Ionicity, Tracer
DiO 484501B, IBPositive Ionicity, Tracer
Dipheny-1-
Hexatriene (DPH)
351430UNeutral
Filipin350 UCholesterol
Fluorescamine390460U, VMembrane Surface Protein
Merocyanine 540500572GMembrane Potential
Organelles 
Acridine Orange490590B, IBLysosome
BODIPY FL503512IBGolgi Apparatus
DiOC6(3)480501IBEndoplasmic Reticulum
Nile Red485-530525-605B, GLysosome
Rhodamine 123500540IBMitochondria
Pyronin Y540570IGMitochondria
Bacteria Staining 
Acridine Orange490530, 640B, IBTubercle Bacilli
Acridine Yellow470550BTubercle Bacilli,
Acid-Fast Bacteria
Auramine O460550BTubercle Bacilli,
Acid-Fast Bacteria
Berberine Sulfate
Hoechst 33258
430550BVGeneral Bacteria
Hoechst 33258365465UMycoplasma Detection
Other Applications 
NBD-Phallacidin465530B, IBActin Fibers
Rhodamine-Phalloidin550580G, IGActin Fibers
Thioflavin T430550BVAmyloid
Table 2

Contributing Authors

Mortimer Abramowitz - Olympus America, Inc., Two Corporate Center Drive., Melville, New York, 11747.

Michael W. Davidson - National High Magnetic Field Laboratory, 1800 East Paul Dirac Dr., The Florida State University, Tallahassee, Florida, 32310.

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