Life Science Solutions

Embryonic Swiss Mouse Fibroblast Cells (3T3)

Embryonic Swiss Mouse Fibroblast Cells (3T3)

In order to examine structural features of the Golgi complex and nucleus at relatively high magnification, a log-phase culture of 3T3 cells was fixed, permeabilized, blocked with normal goat serum, and then treated with rabbit anti-giantin (Golgi protein) primary antibodies followed by goat anti-rabbit secondary antibodies (IgG) conjugated to Alexa Fluor 568. The nuclei were counterstained with Hoechst 33258.

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