생명과학 솔루션

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3D Assays: Intelligent Software, Insightful Analyses

3D Assays: Intelligent Software, Insightful Analyses

In this webinar, we discuss how to obtain the best statistical data for spheroids and microplate-based assays. These data can help you quantify the response of a 3D model to a compound and makes it easier to compare the effect of different treatments, such as varying concentrations.

Presenters:

Daniel Bemmerl, Area Manager, High-End Microscopy Systems
Shohei Imamura, Strategic Project Manager, Regenerative Medicine and Drug Discovery Solutions

FAQ

Webinar FAQs | 3D Analysis

Can NoviSight™ software be used on images obtained with other microscope brands?

Yes, it can. While NoviSight 3D cell analysis software is tailored to Olympus image files, we provide a TIFF image converter for flexibility. As long as your images are in an open format, then you can import them into the software and run the analysis.

Can NovSight software analyze 2D images?

Yes, it can. This capability is useful for broader and more standard applications.

Can widefield microscopes do 3D imaging?

It depends on the sample thickness and required outcome of your experiment. If your sample is thicker than 2–30 μm (i.e., more than 2–3 cell layers) and you want to measure single-cell level information, 3D imaging with widefield microscopy is not recommended as it is hard to visualize the depth due to light scattering.

In contrast, if your sample is less than 2-30 μm or you just want a rough estimation of the size of tissue fragments or clustered cells, such as the volume of whole organoids, it might work depending on your sample specification. Still, we would recommend applying image processing like 3D deconvolution to enhance the contrast. Please keep in mind this is still less accurate than measurement on confocal microscopes, so it is only an intermediate solution.

Can 3D applications be used in fields other than drug discovery?

Yes, definitely. We have seen a strong increase in academic research moving toward 3D data applications. For example, organoids have been studied and used in the regenerative medicine and bioengineering fields.

What microscope is best for 3D live imaging?

A two-photon microscope (also called a multiphoton microscope) is the best fit to observe a 3D live-cell sample such as a spheroid or organoid. Generally, a two-photon microscope is gentler on cells and can observe at depth within a 3D spheroid.

Does NoviSight analysis software incorporate machine learning?

The current algorithms are tailor-made for organoids and spheroids but are also flexible enough to extend to tissues. Machine learning and deep learning might make sense in the future, but for now, voxel analysis works extremely well with the traditional algorithm approach.


관련 제품

3D 세포 분석 소프트웨어

NoviSight

  • 전체 구조에서 아세포까지 3D 이미지 인식
  • 통계 분석
  • 다양한 기본 분석

3D Assays: Intelligent Software, Insightful Analyses

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Webinar: 3D Assays: Intelligent Software, Insightful Analyses

In this webinar, our experts discuss how to obtain the best statistical data for spheroids and microplate-based assays. These data can help you quantify the response of a 3D model to a compound and makes it easier to compare the effect of different treatments, such as varying concentrations.

FAQ

Webinar FAQs | 3D Analysis

Can NoviSight™ software be used on images obtained with other microscope brands?

Yes, it can. While NoviSight 3D cell analysis software is tailored to Olympus image files, we provide a TIFF image converter for flexibility. As long as your images are in an open format, then you can import them into the software and run the analysis.

Can NovSight software analyze 2D images?

Yes, it can. This capability is useful for broader and more standard applications.

Can widefield microscopes do 3D imaging?

It depends on the sample thickness and required outcome of your experiment. If your sample is thicker than 2–30 μm (i.e., more than 2–3 cell layers) and you want to measure single-cell level information, 3D imaging with widefield microscopy is not recommended as it is hard to visualize the depth due to light scattering.

In contrast, if your sample is less than 2-30 μm or you just want a rough estimation of the size of tissue fragments or clustered cells, such as the volume of whole organoids, it might work depending on your sample specification. Still, we would recommend applying image processing like 3D deconvolution to enhance the contrast. Please keep in mind this is still less accurate than measurement on confocal microscopes, so it is only an intermediate solution.

Can 3D applications be used in fields other than drug discovery?

Yes, definitely. We have seen a strong increase in academic research moving toward 3D data applications. For example, organoids have been studied and used in the regenerative medicine and bioengineering fields.

What microscope is best for 3D live imaging?

A two-photon microscope (also called a multiphoton microscope) is the best fit to observe a 3D live-cell sample such as a spheroid or organoid. Generally, a two-photon microscope is gentler on cells and can observe at depth within a 3D spheroid.

Does NoviSight analysis software incorporate machine learning?

The current algorithms are tailor-made for organoids and spheroids but are also flexible enough to extend to tissues. Machine learning and deep learning might make sense in the future, but for now, voxel analysis works extremely well with the traditional algorithm approach.


관련 제품

3D 세포 분석 소프트웨어

NoviSight

  • 전체 구조에서 아세포까지 3D 이미지 인식
  • 통계 분석
  • 다양한 기본 분석
Experts
Daniel Bemmerl
지역 담당 관리자, 고급 현미경 시스템

Daniel Bemmerl입니다. 3D 고함량 분석 및 오르가노이드 이미징 관련 업무를 담당하고 있습니다. TIRF 현미경 및 고함량 스크리닝 응용 전문가로 Olympus에 입사하여 현재 3D 분석 소프트웨어를 전문으로 다루고 있습니다. 분자 및 발달 줄기 세포 생물학을 전공하였으며 이미징을 수행하는 표본보다는 연구의 기술적 측면과 특히 이미징 자체에 더 많은 관심을 가지고 있음을 빨리 깨달았습니다. 실험실 밖에서 작업을 수행하고 고객과 개발 간 교량 역할을 하는 것이 좋아서 Olympus 생명과학에 오게 되었습니다. 3D 이미징에 대한 어떠한 질문도 환영하며 연구에 초점을 맞추도록 이미징 워크플로를 향상시키는 일을 도와 드리겠습니다.

Shohei Imamura
Strategic Project Manager, Regenerative Medicine and Drug Discovery Solutions

Olympus의 전략적 프로젝트 관리자인 Shohei Imamura입니다. 과학 현미경 영업 부문에서 4년, 소프트웨어 관련 제품 기획 부문에서 7년을 종사하였습니다. 전략적 프로젝트 관리 및 실행 부문에서도 종사하고 있습니다. 일본 메이지 대학에서 상업 문학사를 취득하였습니다.

3D Assays: Intelligent Software, Insightful Analyses2021년12월6일
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