Notas de aplicação

Mapping Aortic Valve Cells Using the FLUOVIEW FV3000 Microscope


The valves of a healthy adult heart allow blood to flow to the lungs and throughout the body. However, the structure and function of the aortic valve are vulnerable to environmental stress. Understanding the cellular composition of the aortic valve is critical for the treatment of aortic valvular diseases, such as bicuspid aortic valve calcification and leaflet degradation.

By using the FLUOVIEW FV3000 confocal microscope, we successfully confirmed the contribution of Tie2 positive cells to the aortic valve identified by lineage tracing technology.
 

Easily Perform Macro-to-Micro Imaging of Murine Aortic Valve Cells

Thanks to the 1.25X macro imaging objective, we could easily and quickly acquire a large overview of an aortic valve slice with a single image. By simply dragging the mouse on the overview image, the FV3000 confocal microscope’s macro-to-micro function enabled us to quickly locate target cells and then observe the fine structures in a region of interest using a high-resolution objective lens.
 

Video: Macro-to-micro overview of the expression pattern of Tie2 in the aortic valve of a mouse. Tie2 positive cells are labeled with EGFP (green), and non-endothelial cells are labeled with td Tomato (red).

Imaging equipment
Microscope: FV3000 confocal microscope with a motorized stage
Objective: 1.25X, 10X, and 40X objective (PLAPON1.25X, UPLSAPO10X2, and UPLSAPO40X2)
 

How the FV3000 Confocal Microscope Facilitated Our Experiment

Macro-to-Micro Observation

The FV3000 microscope’s macro-to-micro function provides a roadmap for data acquisition, enabling you to see data in context and easily locate regions of interest for higher resolution imaging.

マクロ~ミクロ観察
 

Extensive Lineup of Olympus Objectives

Olympus offers a wide range of objectives. The FV3000 optical light path supports objectives from 1.25X to 150X and acquires images of samples from large tissues to small organisms.

Objectives line up

Acknowledgments
This application note was prepared with the help of Dr. Wenli Fan, State Key Laboratory of Pharmaceutical Biotechnology, Department of Cardiology, Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University. 

References

  1. Feng, Q. et al. PDK1 regulates vascular remodeling and promotes epithelial-mesenchymal transition in cardiac development. Mol Cell Biol 30, 3711-3721, doi:10.1128/MCB.00420-10 (2010).
  2. Di, R., Yang, Z., Xu, P. & Xu, Y. Silencing PDK1 limits hypoxia-induced pulmonary arterial hypertension in mice via the Akt/p70S6K signaling pathway. Exp Ther Med 18, 699-704, doi:10.3892/etm.2019.7627 (2019).
     

Products used for this application

Microscópio de escaneamento a laser confocal

FV3000

  • Disponível para configurações de escâner híbrido galvanômetro/ressonante (FV3000RS) ou apenas galvanômetro (FV3000)
  • Detecção TruSpectral extremamente precisa e eficiente em todos os canais
  • Otimizado para imagem de célula viva com alta sensibilidade e baixa fototoxicidade
Objetivas macroapocromáticas

PLAPON

  • Estas objetivas exibem imagens planas de fatores de alta transmissão até a região infravermelha próxima do espectro.
  • Adquirem imagens nítidas e claras sem alteração de cor nas observações de fluorescência, campo claro e DIC Nomarski.
  • Oferecem solução imbatível para cada tipo de necessidade de formação de imagem digital de qualidade e desempenho.
Objetivas superapocromáticas

UPLSAPO

  • Fornecem compensação completa para aberrações esféricas e cromáticas vísíveis até a região infravermelha próxima.
  • Adquirem imagens brilhantes e de alta resolução de microscopia de campo claro/fluorescência em ampla faixa de aplicação no campo clínico.

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