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Multiplexing and Deep Tissue Imaging with Near-Infrared Confocal Laser Scanning Microscopy

In this webinar, you’ll learn about fluorescence multiplexing and deep tissue imaging using near-infrared (NIR) laser light. NIR laser sources can help in visualizing biological structures more clearly and at higher resolution deep within a specimen. NIR excitation can also enable the use of more fluorescent dyes without spectral overlap.

Presenters:

Bülent Peker, Senior Product Marketing Manager
Rebecca Bonfig, Product Manager, Confocal Microscopy

FAQ

Webinar FAQs | Near-infrared (NIR) laser microscopy

Do you lose detection of lower wavelengths when using near infrared (NIR) imaging?

Due to the detectors’ design, you retain full availability to detect wavelengths starting at 400 nm. Lower wavelength emissions can still be collected at that range. We still have our standard and GaAsP photomultiplier tubes (PMTs) as well, so NIR is more like an add-on.

What are the benefits of using NIR over two-photon microscopy?

Two-photon microscopy is typically used for high-depth imaging in the red part of the spectrum. NIR offers an easy entry point into this application in a cost-effective way. With NIR, you can use confocal microscopes, so you don’t need to enter into a different class of microscopy system. As well as being much less expensive, NIR also offers greater multiplexing and spectral capabilities.

What is the VPH grating used for inside the microscope?

Volume-phase holographic (VPH) grating is part of our TruSpectral technology in standard and GaAsP detectors. It is used to generate the spectrum of light for spectral imaging. The advantage of VPH grating is that it is much more light efficient than using other reflection-type spectral gratings.

Do I have to purchase NIR lasers with the system or can I upgrade later?

One of the great features of this system is its modularity, so it can be upgraded at any time to add NIR lasers and detectors.

Is the six-detector mode available on both the IX and BX series?

Yes, the six-detector mode is available on both the IX series of inverted microscopes and the BX series of upright microscopes.

Does the FV3000 support NIR excitation wavelengths other than 730 and 785 nm?

The 730 nm and 785 nm laser lines are carefully selected as a result of cooperation with a specialized manufacturer that develops high-quality, stable lasers at these wavelengths.

However, there is a laser port available for the NIR range, so customization is possible. We encourage conversations to discuss the possibility of using different laser lines. The FV3000 microscope’s beam combiner makes it highly amenable to customization, and the system has optical coatings that support wavelengths out to 1600 nm, so users also have the ability to route their own NIR lasers into the system.

Can you image 730 and 785 nm on the same image set?

It is currently not possible to image both 730 nm and 785 nm, but we are always working to improve the system.

What are the differences between GaAsP detectors and competitors’ newest detectors?

We have not compared the systems side by side, but it’s important to remember that the detector is just one part of the whole system sensitivity. Make sure to look at the whole optical train and attached optics and how well they do in the NIR range. We know that there are several aspects during the light path that increase light efficiency in the NIR range, and it is also important that the optics are suited for the NIR range.

Can you use a white light laser with the FV3000 microscope?

White light lasers (WLLs) are not available on the FV3000 system. Olympus has a broad range of laser lines, which allow us to adapt the system for the needs of individual users and match the laser to their applications. There is also a cost-effectiveness factor and the advantage of the whole system not needing to be down if there is a problem with the WLL. Instead, you have individual laser lines that are affordable and easy to change.


相关产品

激光扫描共聚焦显微镜

FV3000

  • 配有常规扫描单元的FV3000或配有常规/共振混合扫描单元的FV3000RS
  • 可在所有通道上进行高效、准确的全真光谱检测
  • 具有针对活细胞成像进行优化的高灵敏度和低光毒性
  • 倒置和正置镜架选项可适应各种应用和样品类型

Multiplexing and Deep Tissue Imaging with Near-Infrared Confocal Laser Scanning Microscopy

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Webinar: Multiplexing and Deep Tissue Imaging with Near-Infrared Confocal Laser Scanning Microscopy

In this webinar, you’ll learn about fluorescence multiplexing and deep tissue imaging using near-infrared (NIR) laser light. NIR laser sources can help in visualizing biological structures more clearly and at higher resolution deep within a specimen. NIR excitation can also enable the use of more fluorescent dyes without spectral overlap.

FAQ

Webinar FAQs | Near-infrared (NIR) laser microscopy

Do you lose detection of lower wavelengths when using near infrared (NIR) imaging?

Due to the detectors’ design, you retain full availability to detect wavelengths starting at 400 nm. Lower wavelength emissions can still be collected at that range. We still have our standard and GaAsP photomultiplier tubes (PMTs) as well, so NIR is more like an add-on.

What are the benefits of using NIR over two-photon microscopy?

Two-photon microscopy is typically used for high-depth imaging in the red part of the spectrum. NIR offers an easy entry point into this application in a cost-effective way. With NIR, you can use confocal microscopes, so you don’t need to enter into a different class of microscopy system. As well as being much less expensive, NIR also offers greater multiplexing and spectral capabilities.

What is the VPH grating used for inside the microscope?

Volume-phase holographic (VPH) grating is part of our TruSpectral technology in standard and GaAsP detectors. It is used to generate the spectrum of light for spectral imaging. The advantage of VPH grating is that it is much more light efficient than using other reflection-type spectral gratings.

Do I have to purchase NIR lasers with the system or can I upgrade later?

One of the great features of this system is its modularity, so it can be upgraded at any time to add NIR lasers and detectors.

Is the six-detector mode available on both the IX and BX series?

Yes, the six-detector mode is available on both the IX series of inverted microscopes and the BX series of upright microscopes.

Does the FV3000 support NIR excitation wavelengths other than 730 and 785 nm?

The 730 nm and 785 nm laser lines are carefully selected as a result of cooperation with a specialized manufacturer that develops high-quality, stable lasers at these wavelengths.

However, there is a laser port available for the NIR range, so customization is possible. We encourage conversations to discuss the possibility of using different laser lines. The FV3000 microscope’s beam combiner makes it highly amenable to customization, and the system has optical coatings that support wavelengths out to 1600 nm, so users also have the ability to route their own NIR lasers into the system.

Can you image 730 and 785 nm on the same image set?

It is currently not possible to image both 730 nm and 785 nm, but we are always working to improve the system.

What are the differences between GaAsP detectors and competitors’ newest detectors?

We have not compared the systems side by side, but it’s important to remember that the detector is just one part of the whole system sensitivity. Make sure to look at the whole optical train and attached optics and how well they do in the NIR range. We know that there are several aspects during the light path that increase light efficiency in the NIR range, and it is also important that the optics are suited for the NIR range.

Can you use a white light laser with the FV3000 microscope?

White light lasers (WLLs) are not available on the FV3000 system. Olympus has a broad range of laser lines, which allow us to adapt the system for the needs of individual users and match the laser to their applications. There is also a cost-effectiveness factor and the advantage of the whole system not needing to be down if there is a problem with the WLL. Instead, you have individual laser lines that are affordable and easy to change.


相关产品

激光扫描共聚焦显微镜

FV3000

  • 配有常规扫描单元的FV3000或配有常规/共振混合扫描单元的FV3000RS
  • 可在所有通道上进行高效、准确的全真光谱检测
  • 具有针对活细胞成像进行优化的高灵敏度和低光毒性
  • 倒置和正置镜架选项可适应各种应用和样品类型
Experts
Bülent Peker
高级产品营销经理

大家好。我是Blent Peker,是您的专职激光扫描显微镜专家。我最初在攻读物理化学博士学位期间对显微镜和光子学产生兴趣,并致力于时间分辨率双光子显微镜的研究。从那时起,我就一直对此充满热情。

我在奥林巴斯工作已超过13年,始终致力于帮助团队将我们最先进的激光扫描显微镜推向市场。我对多光子系统应用以及激光扫描系统的改造可能性深感兴趣。

Rebecca Bonfig
共聚焦显微镜产品经理

大家好。我叫Rebecca Bonfig,是奥林巴斯的共聚焦显微镜产品经理。我的研究生论文在路易斯维尔大学生理学与生物物理学系完成,研究方向为甲状旁腺激素对肾磷酸盐转运蛋白Npt2a的转录后调控。我自2015年起一直在奥林巴斯工作,主要为美国、加拿大和拉丁美洲的FLUOVIEW系列产品提供支持。

Multiplexing and Deep Tissue Imaging with Near-Infrared Confocal Laser Scanning Microscopy2021年10月26日
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