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IXplore TIRF
Excellent multi-color TIRF imaging


The Olympus cellTIRF system provides true simultaneous acquisition of up to four wavelengths. Individual motorized angle control for each laser provides equal evanescent (±1 nm), which enables you to produce high-contrast images with minimal background noise for cell surface and single molecule studies. The cellTIRF 4Line system has integrated point FRAP optics for the first laser line.


The frame architecture and focus drive design of the IX3 system offer enhanced rigidity that reduces the impact of vibration and temperature. It maintains desired positions along the X, Y, and Z axes to facilitate reliable time-lapse and multipoint imaging. When combined with the Olympus ultrasonic stage (IX3-SSU) and Z-drift compensator (IX3-ZDC2), the IX83 microscope can capture high-precision, multipoint time-lapse images that are aligned and in focus. 

Learn more about the IX3-ZDC2

Square frame for increased rigidity.


TIRF is facilitated by a broad lineup of objectives featuring a high signal-to-noise ratio and a correction collar to adjust for cover glass thickness and temperature. Take advantage of Olympus' remarkable TIRF objective with the world's highest NA of 1.7*.

*As of July 25, 2017. According to Olympus research. 

Learn more about the Super High NA Objectives


Olympus’ cellFRAP photomanipulation device and real-time controller (U-RTCE) enable accurate control (200 µs dead time), diffraction-limited stimulation with a flexible region of interest, and precise reproduction of experimental conditions.

Learn more about the cellFRAP

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Advanced functionality, such as object tracking and colocalization, are available with optional cellSens software solutions.


The fast filter wheel, shutter, LED light source control, and real-time controllers (U-RTC) enable less photobleaching and phototoxicity, therefore resulting in healthier cells and more robust data.

Learn more about the U-RTC/E



Y. Yang, et al. Spectraplakin induces positive feedback between fusogens and the actin cytoskeleton to promote cell-cell fusion. Developmental Cell (April 10, 2017).

A. R. van Vliet, et al. The ER stress sensor PERK coordinates ER-plasma membrane contact site formation through interaction with filamin-A and F-actin remodeling. Molecular Cell (Feburuary 23, 2017).

F. Hertel, et al. RefSOFI for mapping nanoscale organization of protein-protein interactions in living cells. Cell Reports (December 31, 2015).

C. Cauvin, et al. Rab35 GTPase triggers switch-like recruitment of the lowe syndrome lipid phosphatase OCRL on newborn endosomes. Current Biology (December 24, 2015).

W.-K. Ji, et al. Actin filaments target the oligomeric maturation of the dynamin GTPase Drp1 to mitochondrial fission sites. eLIFE (November 26, 2015).

A. Juanes-Garcia, et al. A regulatory motif in nonmuscle myosin II-B regulates its role in migratory front–back polarity. The Journal of Cell Biology (April 13, 2015).

D. Borrenberghs, et al. HIV virions as nanoscopic test tubes for probing oligomerization of the integrase enzyme. ACS Nano (March 21, 2014).

S. Yamaoka, et al. Identification and dynamics of arabidopsis adaptor protein-2 complex and its involvement in floral organ development. The Plant Cell (August 23, 2013)

Other Systems

IXplore TOP

IXplore Standard

IXplore Pro

IXplore Live

IXplore Spin

IXplore SpinSR

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