Life Science Solutions

Ask the Experts

Alec De Grand
Alec De Grand

Alec De Grand

产品经理

我叫Alec De Grand,是奥林巴斯虚拟载玻片扫描仪和正置显微镜的产品经理。我在奥林巴斯就职超过10多年,曾负责管理过临床产品、营销计划、显微成像课程和贸易展会。

Bülent Peker

Bülent Peker

高级产品营销经理

大家好。我是Blent Peker,是您的专职激光扫描显微镜专家。我最初在攻读物理化学博士学位期间对显微镜和光子学产生兴趣,并致力于时间分辨率双光子显微镜的研究。从那时起,我就一直对此充满热情。

我在奥林巴斯工作已超过13年,始终致力于帮助团队将我们最先进的激光扫描显微镜推向市场。我对多光子系统应用以及激光扫描系统的改造可能性深感兴趣。

Chunsong Yan

Chunsong Yan

Process Systems业务发展经理

您好,我叫Chunsong Yan,我是奥林巴斯澳大利亚和新西兰公司生命科学业务发展经理。我目前负责共聚焦、多光子、光片和全玻片扫描系统。我于2003年加入奥林巴斯并任过各类职务,任职期间始终致力于为我们的客户提供最佳奥林巴斯解决方案。

Daniel Bemmerl

Daniel Bemmerl

高端显微镜系统的区域经理

我叫Daniel Bemmerl,我可以帮助您了解3D高内涵分析和类器官成像相关的信息。我加入奥林巴斯时担任TIRF显微镜和高内涵筛选的应用专家,目前则专注于3D分析软件。

我曾经从事分子和发育干细胞生物学研究,之后很快意识到相对于处理标本,我对研究的技术层面,特别是成像本身更加感兴趣。之所以加入奥林巴斯生命科学部门,是因为我更喜欢实验室之外的工作,成为沟通客户与开发部门的桥梁。我非常乐意回答您有关3D成像的任何问题,并帮助您改善成像工作流程,让您能够专注从事研究工作。

Nicolas Bourg博士

Nicolas Bourg博士

Abbelight首席技术官兼联合创始人

大家好。我是Abbelight的首席技术官(CTO)兼联合创始人Nicolas Bourg博士,是您的专职单分子定位显微镜(SMLM)专家- 这类显微镜也被称为纳米显微镜-其中包括PALM、dSTORM、SPT-PALM和DNA-PAINT等技术。

我是一名经过培训的光电工程师,并在巴黎-萨克雷大学获得生物光子学研究领域的博士学位,致力于通过独特3D纳米技术实现前所未有的高分辨率的研究。我与我的研究团队创建了Abbelight这家初创公司,决定分享我在博士学位期间获得的所有知识,致力于让纳米显微镜功能更加强大,且让任何生物学家无需进行高级培训即可掌握显微镜的全面操作。我的工作任务是回答您有关纳米显微镜的所有问题,欢迎与我联系。

Flavio Giacobone

Flavio Giacobone

产品营销经理

大家好,我叫Flavio Giacobone,负责奥林巴斯欧洲市场的全玻片扫描解决方案。从攻读生物医学工程专业学位开始,成像始终与我息息相关,加入奥林巴斯让我能够亲身体验到很多临床和科研领域的数字化转变。我从奥林巴斯第一台扫描仪dotSlide开始,就在整个玻片成像方面积累了专业知识,见证了自第一款产品以来所取得的巨大进步。

Ganesh Kadasoor

Ganesh Kadasoor

Olympus Medical Systems India Ltd应用专家

我是Ganesh Kadasoor,过去15年一直参与印度的奥林巴斯高端成像业务。我拥有印度迈索尔大学生命科学学士学位和硕士学位,并获得医学昆虫学的博士学位。我以共聚焦专家身份加入奥林巴斯,负责活细胞成像系统、激光扫描共聚焦、TIRF、转盘共聚焦、超分辨率和多光子显微镜等所有高端成像系统。目前我在班加罗尔的Olympus Medical Systems India Ltd.担任全国经理(应用支持),负责为全国科学家、学者和分销商网络提供培训计划。我还负责组织各种显微镜和成像讲习班、研讨会和网络研讨会,并作为印度讲师/培训员为国家和国际显微镜课程提供支持。

Heiko Gäthje

Heiko Gäthje

培训学院高级培训师

大家好,我叫Heiko Gäthje。我在担任生物学家时开始获得宽视场和共聚焦荧光显微镜以及3D数据图像处理方面的专业知识,当时的研究重点是昆虫的神经元发育和哺乳动物唾液酸结合神经元蛋白质的结构。

我于2004年加入奥林巴斯,自2008年以来一直担任奥林巴斯学院的显微培训师,负责介绍数字学习工具的概念和使用。我还支持并参与EMBL海德堡大学和苏黎世冬季高级显微技术学校举办的显微学培训课程,回答诸多与图像处理和图像分析有关的问题。

Irina Rakotoson

Irina Rakotoson

生命科学产品经理

大家好,我叫Irina,非常期待能够与您一起讨论光片显微镜。我在巴黎笛卡尔大学获得衰老专业的细胞生物学、生理学和病理学硕士学位。在加入PhaseView担任产品经理之前,我曾在神经科学研究实验室(SPPIN)工作,并为显微成像中心平台优化了透明化流程。

Junsung Kim

Junsung Kim

奥林巴斯韩国产品专员

嗨,我叫Junsung Kim,是奥林巴斯韩国公司的共聚焦显微镜产品专家。我拥有高丽大学的生物工程硕士学位。我于2014年加入奥林巴斯,负责为客户提供奥林巴斯共聚焦和多光子显微镜的应用支持。

Kathy Lindsley

Kathy Lindsley

生命科学应用应用专家

我是Kathy Lindsley,是奥林巴斯为基于相机成像系统提供支持的应用专家。我拥有爱荷华州立大学生物化学专业的理学学士学位。我于2006年加入奥林巴斯,担任研究成像销售代表,并于2012年加入生命科学应用团队。在加入奥林巴斯之前,我曾在学术研究领域担任过15年的研究助理,在膜片钳、钙成像、组织培养和免疫组织化学方面积累了丰富的经验。

Lauren Alvarenga

Lauren Alvarenga

生命科学显微镜产品经理

大家好。我是Lauren Alvarenga,在奥林巴斯担任研究成像产品经理,目前负责成像软件以及倒置和超分辨率显微镜。我拥有罗彻斯特理工学院的生物医学摄影通讯专业理学学士学位。
甲状旁腺激素对肾脏磷酸转运蛋白Npt2a的转录后调节。我自2015年起一直在奥林巴斯工作,主要为美国、加拿大和拉丁美洲的FLUOVIEW系列产品提供支持。

Manoel Veiga

Manoel Veiga

生命科学研究应用专家

大家好。我叫Manoel Veiga,是奥林巴斯软件深度学习实施团队成员。我于2017年加入奥林巴斯,拥有高内涵筛选、图像分析和深度学习领域的专业知识。我还是荧光寿命成像的专家。

在攻读物理化学博士学位期间,我开始对数据分析产生兴趣,在了解到卷积神经网络的强大功能及其所能完成难以置信的图像分析任务之后,更加加深了我对这一领域的兴趣。

Minju Kim

Minju Kim

奥林巴斯韩国产品专员

嗨,我是Minly Kim,是奥林巴斯韩国公司生物显微镜的产品专家。我于2010年加入奥林巴斯,担任活细胞和荧光成像系统的高端销售和产品专家。另外,我还负责韩国经销商的培训计划。

Paul Monnier

Paul Monnier

生命科学应用科学家

大家好,我是Paul,非常期待与您讨论CRISPR基因编辑项目。我在巴黎皮埃尔大学和玛丽居里大学获得分子生物学博士学位,并在苏黎世联邦理工学院担任博士后工作,致力于通过CRISPR遗传筛选研究干细胞和X染色体失活。

我曾加入Cytosurge建立过一个新生物实验室,负责开发生命科学领域应用,以及FluidFM技术相关的原理实验和协议验证,目前主要专注于CRISPR应用。如有任何疑问,请随时与我联系。

Rebecca Bonfig

Rebecca Bonfig

共聚焦显微镜产品经理

大家好。我叫Rebecca Bonfig,是奥林巴斯的共聚焦显微镜产品经理。我的研究生论文在路易斯维尔大学生理学与生物物理学系完成,研究方向为甲状旁腺激素对肾磷酸盐转运蛋白Npt2a的转录后调控。我自2015年起一直在奥林巴斯工作,主要为美国、加拿大和拉丁美洲的FLUOVIEW系列产品提供支持。

Shohei Imamura

Shohei Imamura

战略项目经理

我叫Imamura Shohei,是奥林巴斯的战略项目经理。我在科学显微镜的销售方面拥有4年经验,并在侧重软件的产品规划方面拥有7年经验。我还负责战略项目管理和执行。我拥有日本明治大学的商业学士学位。

Srivats Hariharan

Srivats Hariharan

奥林巴斯新加坡公司产品应用经理

大家好!我叫Srivats Hariharan aka Hari,是新加坡奥林巴斯的共聚焦、多光子和超分辨率显微镜产品经理。我拥有新加坡南洋理工大学的机械工程学士学位,并在生物医学研究实验室和A*STAR显微镜核心设施工作,在此期间,我为研究人员提供共聚焦和活细胞成像技术方面的支持,并帮助设置单分子超分辨率和光片显微镜。
我于2011年加入奥林巴斯新加坡生命科学团队,担任产品经理,负责支持东南亚和台湾地区的科研客户和业务合作伙伴。

Stefan Marawske

Stefan Marawske

高端生命科学系统高级销售专员

您好,我叫Stefan Marawske,是您的超分辨率显微镜专家。在攻读物理化学领域博士学位期间,我曾制作过用于基于定位超分辨率和粒子跟踪的自搭建显微镜。我特别沉迷于那些能够突破著名的阿贝极限以及能够分辨以前无法观察结构的方法。

我在奥林巴斯工作了超过7年,主要负责TIRF和转盘等高端成像系统。这类系统通常由于能够将许多不同设备组合用于各种应用而具有高度的灵活性,因此,如果您在确定专用配置方面需要任何帮助,欢迎与我们联系。

Takeo Ogama

Takeo Ogama

高级产品和策略规划师和产品经理

我叫Takeo Ogama,是奥林巴斯显微镜相机的高级产品和策略规划师兼产品经理。我在相机等各种产品的研发部门拥有八年的工作经验,也在产品规划、营销和管理方面拥有八年的经验。加入奥林巴斯之前,我从日本大阪大学获得中微子物理学的硕士学位。

Wei Juan Wong

Wei Juan Wong

奥林巴斯新加坡高级产品专员

大家好!我叫Wei Juan Wong,是临床和研究市场产品的产品专家。我拥有物理学学位,并且在生物物理研究实验室以及显微镜中心平台均具备丰富的工作经验。我于2018年加入奥林巴斯新加坡生命科学团队,担任产品专家,为客户提供应用支持并负责东南亚地区代理商的培训计划。

Klaus Willeke

Klaus Willeke

Product Marketing Manager

Hello, I’m Klaus Willeke, Product Marketing Manager at Olympus Life Science Division and I’m responsible for our new X Line objectives. During my geology studies, the polarization microscope was an essential tool for determining and researching mineral compositions and structures. I was always fascinated by how colorful the world of minerals appears through a polarization microscope and how much you can discover with the help of light and good optics.

I’ve been working for Olympus for more than 22 years where I was 17 years a sales representative for industrial and life science microcopy in Germany and after that in European Product Marketing in the Scientific Solutions Division of Olympus, responsible for upright clinical and research microscopes and X Line lenses. 

Dr. Hrishikesh Pai

Dr. Hrishikesh Pai

Medical Director, Bloom IVF Group

Dr Hrishikesh Pai did his Fellowship in Reproductive Biology from the Royal Women’s Hospital, Melbourne Australia in 1989. He did his Masters in Clinical Embryology and Andrology from the Jones Institute, Eastern Virginia Medical School, USA in 2006. He has won 45 plus awards including the Honorary FRCOG from the Royal College of Obstetricians & Gynecologists, United Kingdom in 2019. Dr Pai has been the Past President of the Indian Society for Assisted Reproduction (ISAR) and is the present Director of Corporate Affairs for International Federation of Fertility Societies (IFFS). Dr Pai is the President Elect of the Federation of Obstetric and Gynecological Societies of India (FOGSI) with a membership strength of 37,000 members. It is the second largest medical body of gynecologists in the world, only second to the ACOG.

Hiroya Ishihara

Hiroya Ishihara

Applications Scientist

Mr. Hiroya Ishihara is an Application Scientist at Olympus. He was studying the epigenetic factors involved in plant regeneration using omics and microscopy in Tokyo University of Science. Confocal and two-photon microscopy were his trusted partner at that time. Therefore, he joined Olympus to make life science more exciting with microscopes. Currently, he is working on a wide range of projects from basic research to product/sales strategy.

Dr. Gowri Balachander

Dr. Gowri Balachander

Research Fellow, Translation Mechanobiology lab, National University of Singapore

Dr. Gowri Balachander is a Research Fellow at the Yong Loo Lin School of Medicine, National University of Singapore. She completed her PhD at the prestigious Indian Institute of Science, Bangalore, India where she worked on engineering 3D organotypic models for the study of breast cancer metastasis. At NUS, she currently works on morphogenetic models for human liver development and regeneration.

Joanna Hawryluk

Joanna Hawryluk

Product Manager

Joanna Hawryluk is a product manager for Olympus Corporation of the Americas located in Waltham, MA. She has been with Olympus since 2017 within the Marketing department specializing in our 3D cell analysis software, lightsheet microscopy, electrophysiology, and our cell culture monitoring system. Dr. Hawryluk received her doctorate degree in Physiology and Neurobiology in 2016 from the University of Connecticut.

Benjamin Compans, Ph.D.

Benjamin Compans, Ph.D.

Marie Curie Research Fellow

Benjamin received his Ph.D. from the University of Bordeaux. During his Ph.D., in the group of Dr. Choquet at the Interdisciplinary Institute for Neuroscience, he studied the nanoscale organization of glutamate receptors at excitatory synapse and its importance for synaptic plasticity. In 2018, he joined the lab of Professor Burrone at King’s College London for his postdoc where he investigates the molecular organization of inhibitory synapses and its role in regulating neuronal firing, a project for which he obtained a Marie Sklodowska-Curie Action postdoctoral fellowship.

Lin Guo

Lin Guo

Manager, Product and Application Life Science Department Scientific Solutions Business Division

Lin got his PhD back in 2010 in National University of Singapore working on biophysical research. From 2009 he joined Olympus as Technical and Application specialist taking care of laser based high end imaging system. In 2012, Lin decided to move back to China and taking a position with one of the leading scientific camera manufacturers Photometrics. There, he started as application specialist, later become regional sales manager and finally scientific sales manager for Asia Pacific. In 2021, Lin moved back to Singapore, joining Olympus Singapore as the manager for product and application. Lin has a long experience of various techniques on scientific digital imaging including various camera technologies.

Angela Vasaturo

Angela Vasaturo

Senior Field Application Scientist, Ultivue, Inc.

Hello, my name is Angela Vasaturo, the senior field application scientist at Ultivue. My passion for micro-biology and live-cell imaging began during my post-doc, where I was involved in the early development of multiplex IHC in Europe.
I built my expertise through extensive involvement in post-doc research focused on tumor immunology at the NCMLS in Nijmegen, NL, as well as during my position as Senior Researcher in Dr. Jerome Galon’s Laboratory of Integrative Cancer Immunology at the Cordeliers Research Center.

Shogo Usui

Shogo Usui

Product Leader

My name is Shogo Usui and I'm product leader of CM20 at Olympus Corporation.

For over ten years, I have been an electrical engineer in the life science R&D team in Olympus Tokyo. I hold a Master’s degree in applied physics from the University of Electrical and Communication from Tokyo, Japan.

Dr. Anne Beghin

Dr. Anne Beghin

Assistant Professor, Research Mechanobiology Institute

Dr. Anne Beghin is a multidisciplinary scientist with fifteen years of extensive research experience across academia and industry. She obtained her PhD in oncology in 2007 at the University Claude Bernard in Lyon (France). She then moved to optical microscopy at the Université de Lyon, where she established the microscopy platform and developed live cell imaging solutions and image analysis services for 4 years.

In 2011, she was recruited by a biotechnology company based in Bordeaux, where she spent 3 years in charge of a tissue analysis service: from biologic samples (whole tissue sections and Tissue Micro Arrays) to image acquisition and analysis with database establishment. She has been part of the Interdisciplinary Institute of NeuroScience IINS for 3 years where she successfully developed a new platform linking the High Content Screening (HCS) approach with super resolution microscopy such as Single Molecule Light Microscopy (HCS-SMLM), a collaboration with pharmaceutical company, Sanofi. Subsequently, she moved to the MechanoBiology Institute (MBI) in Singapore to study organoids using advanced imaging and HCS. This work has resulted in a patent and publications are on-going.

Dr. Xiaotong Cui

Dr. Xiaotong Cui

Field Application Specialist

Dr. Xiaotong Cui received his B.S. and M.Sc. from the University of Leicester, UK. He went on to receive aPh.D. from the Institute of Life Science, Kyoto University in 2018, where he worked under the direction of Prof. Osamu Takeuchi. From 2018-2020, he was a program-specific researcher in Shenzhen Digital Life Institute and ASHBi, Japan. Dr. Xiaotong has a solid background in molecular cell biology, immunology and he participated in one special program for Key Basic Research of Ministry of Science and Technology, China. Now, he serves as a Field Application Specialist in Bio-Techne China.

Dr. Kasmira Wilson

Dr. Kasmira Wilson

Researcher

Dr. Kasmira Wilson is a general surgeon and CSSANZ trainee, having previously completed a BSc(Hons) and MBBS. She is currently undertaking a PhD through the University of Melbourne at Peter MacCallum cancer centre which focuses on translational research in rectal cancer. Her research utilises tumouroid models to explore anti-tumour immunity in rectal cancer in a novel functional cytotoxic assay.

Dr. Dan Zhu

Dr. Dan Zhu

Professor

Dr. Dan Zhu is a professor of Huazhong University of Science & Technology, and Vice-Director of Wuhan National Laboratory for Optoelectronics. Her research interests mainly focus on tissue optical clearing imaging and applications. She is the pioneer in the field of in vivo tissue optical clearing, and also developed fast, label-compatible in vitro optical clearing methods. She has authored more than 150 papers including Science Advances, Nature Communications, et al. She is also Fellow of SPIE, and Secretary General & Vice President of Biomedical Photonics Committee of Chinese Optical Society. She serves for journals as editorial member or guest editor, including Biomedical Optics Express, Journal of Biomedical Optics, Scientific Reports, Journal of Innovative Optical Health Sciences, Frontier of Optoelectronics et al.

Dr. Graham Wright

Dr. Graham Wright

Acting Director

Dr. Graham Wright holds an interdisciplinary PhD in cell biology and physics from The University of Edinburgh and an MBA from the Warwick Business School at The University of Warwick. He is the Acting Director of A*STAR’s Research Support Centre (RSC) and the Director of the A*STAR Microscopy Platform (AMP). RSC offers everything from on-demand access to sophisticated scientific instruments and services, located within varied Technology Platforms, to a research consumables webstore, playing a crucial role in powering biomedical innovation in Singapore.

Dr. Graham has extensive experience, and a strong publication record, in applying advanced light microscopy to a wide range of biomedical research projects. Outside the laboratory, he is committed to science outreach and has featured as a judge on MediaCorp’s National Science Challenge TV show, presented a TEDx talk, had microscopy images displayed on the big screen in Times Square, New York and exhibited work at the National Museum of Singapore.

Mr. Srivats Hariharan

Mr. Srivats Hariharan

Manager, Applications and Marketing

Mr. Srivats Hariharan is an Applications & Marketing Manager in Olympus life science team in the Asia Pacific region. He holds a bachelor’s degree in Mechanical Engineering from Nanyang Technological University, Singapore and has experience working in biomedical research labs and A*STAR Microscopy Core Facility where he supported researchers on confocal and live cell imaging technologies and help setup single molecule super-resolution and light sheet microscopes. He joined the life-science team of Olympus Singapore in 2011 as Product Manager and in-charge of supporting research customers and business partners in South-East Asia and Taiwan.

Ms. Gency Gunasingh

Ms. Gency Gunasingh

Research Assistant

Ms. Gency Gunasingh completed her Master of biotechnology degree from University of Queensland in 2012 and did her Masters project under Dr. Andrew Prowse and Prof. Peter Gray at Australian Institute for Bioengineering and Nanotechnology. Her primary area of research was large scale generation of cardiac progenitors from embryonic stem cells in 3D. She then worked under Prof. Brian Gabrielli at UQ Diamantina institute on developing 3D tumour spheres in melanoma for in vitro drug testing. She currently works for Prof. Nikolas Haass at UQDI on understanding tumour heterogeneity and tumour architecture in melanoma spheroids.

Dr. Dong Gao

Dr. Dong Gao

Principal Investigator

Dr. Dong Gao is the Principal Investigator of Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences. His research interests mainly focus on t prostate adult stem cell and the establishment of patients-derived cancer organoid biobank. He has authored more than 40 papers including Cell, Nature Genetics, Cell Stem Cell et al.

Dr. Yu Weimiao

Dr. Yu Weimiao

Head of Computational & Molecular Pathology Lab (CMPL) Agency of Science

Dr. Yu Weimiao obtained his Ph.D. from the National University of Singapore (NUS) in 2007, majoring in image processing and machine vision. He joined the Agency of Science, Technology and Research (A*STAR) in 2007. He is currently heading Computational Digital Pathology Lab (CMPL) in BII to deepen and extend the R&D with clinical and industrial partners. He is also the joint PI in IMCB leading the Computational & Molecular Pathology Lab (CMPL). His research interests are Computational Biomedical Image Analysis and Quantitative Imaging Informatics. He applied 3D image analysis solution to segment and tracking the cells in 3D to understand the developmental problem and collective cell migration mechanisms. His work was published in Nature Communication, Current biology, Nature Cell Biology, etc. To enhance the applications in clinical diagnosis/prognosis, he co-founded a biotech company, known as A!maginostic Pte. Ltd. He established a world-class joint platform for the immunodiagnosis at the tissue level. The platform allows the researchers, clinicians, and pharma to profile the patient immune signature for diagnosis, prognosis, and drug response study.

Dr. Motoki Takagi

Dr. Motoki Takagi

Professor

Dr. Motoki Takagi received PhD from Graduate School of Agriculture and Life Sciences, the University of Tokyo in 2001. Then he continued his research as a postdoctoral fellow at Institute of Molecular and Cellular Biosciences, the University of Tokyo. He was engaged in the research of nucleic acid drugs at Genencare Research Institute, Co. Ltd. since 2002. Since 2006, he conducted drug discovery research at Biological Systems Control Team, Biomedicinal Information Research Center, Japan Biological Informatics Consortium. Since 2012, he has been researching chemical biology as an associate professor at Fukushima Medical University and became a professor in 2014.

Dr. Ningbo Wu

Dr. Ningbo Wu

Associate Professor

Dr. Ningbo Wu is an Associate Professor at Shanghai Institute of immunology, Shanghai Jiaotong University School of Medicine. His research interests mainly focus on the function of intestinal stromal cells in intestinal homeostasis and related work was published in Nature and Science CHINA Life Sciences, et al.

Dr. Nuno Costa-Borges

Dr. Nuno Costa-Borges

Embryologist and Co-founder of Embryotools

Hi, I’m Nuno Costa-Borges, embryologist and co-founder of Embryotools - a company devoted to offering QC tests, training and consulting services to the global IVF community. My 18 years of experience and passion for this field began during my PhD on animal cloning efficiency, and further developed when I joined IVI Barcelona as a clinical embryologist.

Now, as scientific director of Embryotools, I am committed to developing new IVF techniques including maternal spindle transfer for infertility - leading to the world’s first baby via this technique.

Akira Saito

Akira Saito

Assistant Manager, Marketing and Applications

Akira studied veterinary medicine at Tokyo University of Agriculture and Technology, Japan and graduated in 2007. Shortly after, he joined Olympus as application specialist responsible for in vivo imaging systems, high-content analysis systems, and laser confocal systems to support customers in Japan. In 2013, he took over sales promotion for all Olympus life science products. From 2018, he moved to Singapore and joined to support the marketing and application support for the APAC market.

Bob McLean

Bob McLean

Regents’ Professor

Bob McLean has over 30 years’ experience as a microbiologist, during which time he and his lab have done a number of studies on surface-adherent microorganisms (biofilms). In 1998, he and his colleagues had an experiment on the space shuttle with John Glenn, in which they were one of the first research groups to show that biofilms could form in microgravity. Since that discovery, there have been a number of biofilm issues, notably instances of fouling in the water recovery system in the International Space Station and other spacecraft. In 2015, Bob, along with collaborators at Arizona State and the Johnson Space Center, received a NASA grant to study biofilm formation during spaceflight. Confocal and other types of microscopy have been instrumental in these investigations.

Jesse Chao

Jesse Chao

Scientist

Jesse completed his Ph.D. at the University of British Columbia (UBC) in cell biology and genomics. He then continued his training at the University of California, San Diego. After, he switched his focus to developing machine learning approaches for assessing the physiological impacts of genetic variants associated with hereditary cancer at UBC. During this time, he started to develop deep learning approaches to automated phenotypic profiling based on high-content imaging data.

James Lopez

James Lopez

National Applications Manager

James Lopez received his Ph.D. in biomedical sciences from the University of Chicago in 2010. With nearly a decade of experience in calcium imaging, FRET, live cell imaging, and intravital imaging, James joined Olympus as a confocal and multiphoton sales representative. He later transitioned to the Olympus Life Science Applications Group, supporting confocal and multiphoton systems. Now he manages the Life Science Applications Group in the US, Canada, and Latin America markets.

Yosuke Yoneyama

Yosuke Yoneyama

Assistant Professor

Yosuke Yoneyama obtained his Ph.D. from The University of Tokyo in Japan, where he continued his post-doctoral work on insulin/insulin-like growth factor signaling with a focus on spatiotemporal control of the intracellular signaling system in the laboratory of Dr. Shin-Ichiro Takahashi. He then joined the laboratory of Dr. Takanori Takebe at Tokyo Medical and Dental University in Japan as an assistant professor. He now focuses on human organoids, in particular liver organoids, that are derived from stem cells, including induced pluripotent stem cells (iPSCs), to reconstitute multiple lineages of liver cells both for human development and modeling diseases such as non-alcoholic steatohepatitis.

Ewa Goldys

Ewa Goldys

Deputy Director

Professor Ewa M. Goldys is Deputy Director of the Australian Research Council Centre of Excellence in Nanoscale Biophotonics (cnbp.org.au) and Professor at the Graduate School of Biomedical Engineering, the University of New South Wales, Sydney, Australia.  She is Fellow of SPIE, OSA, the Australian Academy of Technological Science and Engineering (ATSE), and winner of the 2016 Australian Museum Eureka Prize for ‘Innovative Use of Technology.’ She has ongoing involvement with SPIE BIOS, the world's largest international biomedical optics meeting and SPIE's Photonics West where she serves as Track Chair in Nanobiophotonics.

Her research spans the areas of biomedical science, bioimaging, biosensing, and materials science. She developed novel approaches to biochemical and medical sensing and deployable medical diagnostics. Current projects focus on cancer nanotechnology and non-invasive high-content imaging of colors and patterns in cells and tissues.

Laura Vittadello

Laura Vittadello

Department of Physics and Center of Cellular Nanoanalytics

Dr. Laura Vittadello is working as a post-doc in the physics department of the Osnabrück University in the ultrafast physics research group. Her research focus is on the fundamental study and application of a new type of marker, harmonic nanoparticles, that are specially designed for biological applications that involve nonlinear microscopy.

Francesco Cardarelli

Francesco Cardarelli

Associate Professor in Applied Physics

After receiving his M.Sc. in Biological Sciences from the University of Pisa in Oct 2003 and his Diploma in Biological Sciences in the same year (both with honors) from SNS, Francesco Cardarelli worked at the NEST Laboratory of SNS as a Ph.D. student in Molecular Biophysics under the supervision of Prof. Fabio Beltram. He started his interdisciplinary research at the crossroads between cell biology and physics, using advanced fluorescence microscopy methods to study the intracellular transport properties of virus-derived peptide sequences. After graduating, he became a Post-Doctoral Fellow at the Laboratory for Fluorescence Dynamics, University of California at Irvine, under the supervision of Prof. Enrico Gratton, where he coordinated the research activity for the development of new spatial variants of fluorescence correlation spectroscopy to detect barriers to molecular diffusion/flow in live cells. In Dec 2010 he was hired by the CNI@NEST (IIT) as a Post-Doctoral Fellow. Back in Italy, he started working to develop new fluorescence-based imaging and analysis methods to study single molecules in complex biological systems with high spatiotemporal resolution. This research was boosted by a number of funded grants (and established collaborations) and by an independent scientific position, first at CNR as a Researcher, then at SNS as Professor in Applied Physics.

The focus of his research is on the development of new optical microscopy techniques to increase the amount of quantitative information that can be extracted from investigations on living matter. For instance, in recent years, he and his team introduced a number of new spatiotemporal fluctuation-analysis tools (iMSD, iRICS, nD-pCF, diffusion tensor analysis, etc.) to extract structural and dynamic properties of biological objects, from molecules to entire sub-cellular structures, in their complex natural environment. Such a toolbox is becoming a new paradigm for biophysical investigations at the nanoscale, as featured in the “New and Notable” section of Biophysical Journal (2016 Aug 23; 111(4): 677–678). In 2014, together with his Team, they demonstrated the occurrence of short-range protein Brownian motion in the cell cytoplasm, being among the first to challenge the current view of the structural organization of the crowded intracellular environment. Finally, by combining this toolbox with feedback-based orbital tracking, they demonstrated that even the nanoscopic and dynamic environment of intracellular organelles can be quantitatively probed.

Sandrine Roy

Sandrine Roy

Business Development Manager

Dr. Roy completed a double-major in Biochemistry and Microbiology followed by the completion of a Doctor of Philosophy in 2002 in the field of Molecular Biology/Cell Biology at the University of Queensland Australia. She travelled abroad to undertake a post-doctoral position at Washington University in St Louis, USA. She then returned to Australia to continue her post-doctoral studies.

With her extensive microscopy experience, she was appointed as the University of Queensland Diamantina Institute Microscopy Facility Manager in 2009 and later as manager of microscopy services at the Translational Research Institute in Brisbane until 2019.

She is now Business Development Manager at Olympus Australia, where her experience and knowledge is used to support customers both in Australia and New Zealand.

Seungil Kim

Seungil Kim

Staff Scientist

Seungil Kim, Ph.D., is a Staff Scientist and Microscopy Team Manager at the Lawrence J. Ellison Institute for Transformative Medicine at USC. Dr. Kim completed his B.S. and M.S. degrees in South Korea. He then moved to Washington University and received a doctoral degree in Developmental Biology. He carried out his postdoctoral research in the department of Cell and Tissue Biology at UCSF. Seungil has over 10 years of experience working with various in vitro/in vivo models and advanced cellular imaging techniques. His current research focus is to understand the contributions of the tumor microenvironment to drug response, using patient-derived 3D organoids as a model system. Moreover, he is developing high-throughput automated imaging methods to screen novel drug compounds in colorectal cancer.

Alfonso J. Schmidt

Alfonso J. Schmidt

Senior Staff Scientist

Alfonso has a decade of experience working in a shared resource lab (SRL) with a vast knowledge in histology, fluorescent microscopy, and image analysis. His work has been focused in maximizing the capabilities of the equipment available and in creating technical protocols and training modules for the scientific community. Currently, Alfonso oversees the Histology and Bioimaging Facility as part of the Hugh Green Cytometry Centre (HGCC) at the Malaghan Institute of Medical Research in Wellington, New Zealand.

Tong Wu

Tong Wu

Business Development Manager

Tong Wu joined Olympus in 2012 after completing her Ph.D. in China (State Key Laboratory of Fine Chemicals, DLUT). Now, Tong is a business development manager, supporting high-end microscopes in Olympus Australia. With a research background in fluorescent dyes for bio-imaging and bio-labelling, Tong Wu is enthusiastic to engage with customers’ research applications.

Ruben Portugues

Ruben Portugues

Institute of Neuroscience

Prof. Portugues is a neurobiologist studying sensorimotor control. His research group uses behavior, modeling, optogenetics, in vivo electrophysiology, and whole brain functional calcium imaging to dissect learning, memory, and action selection in the larval zebrafish.

Prof. Portugues studied mathematics and did his Ph.D. in theoretical physics at Trinity College in the University of Cambridge. After a short postdoctoral fellowship in physics at Centro de Estudios Cientificos in Valdivia, Chile, he joined Professor Florian Engert’s laboratory at Harvard University and switched research interests to neuroscience. In 2014 he was appointed Max Planck Research Group Leader at the Max Planck Institute of Neurobiology in Martinsried. Since 2020 Prof. Portugues is an assistant professor at the TUM.

Ask the Experts

对多个亚细胞结构进行纳米级 3D 成像

对多个亚细胞结构进行纳米级 3D 成像

Experts
Nicolas Bourg博士
Abbelight首席技术官兼联合创始人
加入 Abbelight 联合创始人 Nicolas Bourg 博士的行列,看他阐述以 15 nm 分辨率获取高质量、可靠且易于理解的单分子 3D 图像有多么快速和轻松。

Whole-Brain Functional Calcium Imaging Using Light Sheet Microscopy

Experts
Ruben Portugues
Institute of Neuroscience
Technical University of Munich
Light sheet microscopy is a powerful technique to perform fast volumetric imaging. I will talk about how we use it to investigate how the brain of a small vertebrate, the larval zebrafish, works. In our laboratory at the Technical University of Munich, we are interested in how the brain processes external sensory stimuli and uses internal states and past experiences to select appropriate behavior. In order to do this, we image the activity of almost all 100,000 neurons in the brain of larval zebrafish while we present the animals with stimuli and monitor their behavior. I will also discuss the data processing steps after acquiring these large datasets.

Product Demo: SLIDEVIEW™ VS200 Research Slide Scanner

Experts
Wei Juan Wong
奥林巴斯新加坡高级产品专员
Olympus Soft Imaging Solutions
In this product demo you will learn how to capture high-resolution images of your slides for quantitative analysis, enabling you to make the most of the information your slides have to offer. Easily analyze, share, and archive your data with the SLIDEVIEW VS200 digital slide scanner. Join this session and learn how to achieve more in less lead time.

Product Demo: SLIDEVIEW™ VS200 Research Slide Scanner

Experts
Chunsong Yan
Process Systems业务发展经理
Olympus Australia & New Zealand
Tong Wu
Business Development Manager
Olympus Australia New Zealand
The Olympus SLIDEVIEW VS200 research slide scanner captures high-quality virtual slide images and enables advanced quantitative image analysis. Reliable virtual slide data can be acquired with as few as two clicks. Highly versatile, the SLIDEVIEW VS200 slide scanner supports five observation methods and a wide range of sample sizes for use in various applications. Its automatic slide loader accommodates many slide glasses, helping increase experiment efficiency.

The Use of Multiplexing in Microscopy for Better Understanding the Skin Immune System in the Context of the Tissue

Experts
Alfonso J. Schmidt
Senior Staff Scientist
Malaghan Institute of Medical Research
The skin is the first line of defense and the immune system’s biggest barrier for combating pathogens. Being able to accurately characterize and identify immune cell subtypes, tissues structures, and cell distribution in the skin under steady-state conditions provides a powerful tool for understanding the first immunological strategies and biological processes that occur in the presence of pathogens. In this webinar we will review technical aspects involved in the experimental process and explore how complementary imaging technologies might assist us to better understand the immune system. The presentation is divided into three parts. First, an introduction of the Hugh Green Cytometry Centre will be presented and an overview of the histology and bioimaging technological platforms available. Second, the multiplexing methodology will be discussed, where several topics need to be considered for the design and development of a successful polychromatic panel for microscopy. Finally, preliminary results from a research project will be presented that constitutes part of a diploma program from The Royal Microscopical Society. The project focuses on the identification of immune cell types in the whole mount skin in relation to tissues structures (e.g., blood vessels and lymphatic network). It also centers on the immune cells’ distribution in the tissue as a first barrier of defense against pathogens.

Recent Advances in 3D Imaging and AI-Driven Data Analysis

Experts
Seungil Kim
Staff Scientist
Ellison Institute for Transformative Medicine at the University of Southern California
This presentation will highlight various imaging techniques for 3D models, immunostaining with tissue clearing, and live imaging of organoids as well as AI-driven data analysis for high-content imaging and screening.

Now You Have the Power to See More

Experts
Sandrine Roy
Business Development Manager
Olympus Australia & New Zealand
The Olympus VS200 digital slide scanner has been very well received since its release in March 2020. With a reliable, flexible, and customizable design, the system has been adopted by various industries including research, geology, and many others. View this session to find out more and see examples of samples scanned using this popular addition to the Olympus product range.

Metabolic Imaging in Langerhans Human Islets with MPE and FLIM

Experts
Francesco Cardarelli
Associate Professor in Applied Physics
Scuola Normale Superiore of Pisa
Capturing life (mis)regulation at the nanoscale is a crucial challenge for present and future biophysics. At this scale, the main actors are the molecules. To successfully tackle molecular behavior within living matter, optical microscopy is a valuable methodological platform: by using fluorescence as the signal, spatial and temporal details of molecular processes can be investigated quantitatively. The physiopathology of beta-cell response to glucose stimulation will be used as case study of biological/biomedical interest. The metabolic traits of beta cells will be highlighted using a straightforward combination of multiphoton microscopy, fluorescence lifetime imaging, and feedback-based orbital tracking of sub-cellular nanostructures.

Product Demo: IXplore™ SpinSR Confocal Super Resolution System

Experts
Stefan Marawske
高端生命科学系统高级销售专员
Olympus Europa
In this live demo, experience the IXplore SpinSR system, designed for fast 3D super resolution imaging and prolonged cell viability in time-lapse experiments. The microscope system offers XY resolution down to 120 nm without the need for dedicated labeling procedures. Learn how to easily integrate the IXplore SpinSR microscope system into existing experiments and sample protocols to streamline your research.

In-Vivo Tracking of Harmonic Nanoparticles by Means of a TIGER Widefield Microscope

Experts
Laura Vittadello
Department of Physics and Center of Cellular Nanoanalytics
Osnabrück University
In-vivo tracking based on harmonic nanoparticles is so far not exploited because of a lack of an appropriate tool—a widefield nonlinear optical microscope. Here, we present a new approach to overcome this challenge based on a redesign of laser space parameters.

Hyperspectral and Brightfield Imaging Combined with Deep Learning Uncover Hidden Regularities of Colors and Patterns in Cells and Tissues

Experts
Ewa Goldys
Deputy Director
Australian Research Council Centre of Excellence in Nanoscale Biophotonics
The Australian Research Council Centre of Excellence for Nanoscale Biophotonics draws on key advances of the 21st century, nanoscience, and photonics to help understand life at the molecular level. In this presentation, next-generation technologies developed in our Centre for probing, imaging, and interacting with the living systems will be discussed. These address the key challenges of ultrasensitive detection of key analytes in real complex environments and molecular complexity, and they support both novel therapies and diagnostics.

Product Demo: FLUOVIEW™ FV3000 Confocal Laser Scanning Microscope

Experts
James Lopez
National Applications Manager
Olympus America Inc.
Join James Lopez, PhD, National Applications Manager to see how the FV3000 confocal laser microscope can expand your research possibilities and help you get more data from your samples.

Product Demo: FLUOVIEW™ FV3000 Confocal Laser Scanning Microscope

Experts
Bülent Peker
高级产品营销经理
Olympus Europa
Join Bülent Peker, Senior Product Marketing Manager to see how the FV3000 confocal laser microscope can expand your research possibilities and help you get more data from your samples.

Evolution of Scientific Digital Imaging Technologies and their Applications

Experts
Lin Guo
Manager, Product and Application Life Science Department Scientific Solutions Business Division
Olympus Singapore
In this talk, Dr. Lin covers some critical facts about scientific digital cameras. He also discusses the evolution of these cameras, the solutions that Olympus offers, and how they are used in current advanced microscopy systems for various applications.

Deep Learning Approaches to Automated Phenotypic Profiling

Experts
Jesse Chao
Scientist
Sunnybrook Research Institute
Quantifying cellular phenotypes is the key to all cell biology studies. However, modern imaging techniques can easily generate more data than an average user can comfortably handle. In this presentation, Dr. Chao discusses two deep learning approaches, one semi-supervised and one supervised, for building image analysis pipelines. Either approach can be run on a free cloud GPU instance.

Deconvolution of 3D Image Stacks

Experts
Heiko Gäthje
培训学院高级培训师
Olympus Europa
Images taken with a light microscope are never true representations of the specimen. Error sources, which have to be controlled, are sample preparation and staining protocols as well as optical aberrations and limitations of microscope and digital camera.

Confocal Microscopy and Its Use for a Spaceflight Experiment

Experts
Bob McLean
Regents’ Professor
Texas State University
Spaceflight experiments represent a rare but exciting scientific opportunity. Unlike most lab experiments, in which protocols can be quickly modified, limitations on crew time and availability of supplies are notable factors. Unanticipated changes to launch and reentry schedules are also an issue. The experimental apparatus and protocols used must be able to function in a microgravity setting, while also resisting the g-forces and vibrations during launch and landing. During this presentation, Dr. McLean will go over the experimental planning and use of confocal and electron microscopy approaches and analyses during a recent spaceflight experiment that flew on Space X-21 from 12/6/20 – 1/14/21.

Accelerating Image Analysis with TruAI™ Deep Learning Technology

Experts
Manoel Veiga
生命科学研究应用专家
Olympus Soft Imaging Solutions
In this tech talk, through a collection of examples measured with our live cell imaging systems, high-content screening station, and whole slide scanner, you will see what TruAI technology can do for your research and get a preview of what is coming next.

A New Way of Thinking—Object Detection with Deep Learning

Experts
Akira Saito
Assistant Manager, Marketing and Applications
Olympus Singapore
In this session, we will discuss object segmentation with deep learning and its applications in life science. We will also demo Olympus deep-learning software.

ICSI - How to improve your technique

Experts
Dr. Nuno Costa-Borges
Embryologist and Co-founder of Embryotools
In this webinar, expert embryologist Nuno Costa-Borges will discuss how current success rates of intracytoplasmic sperm injection (ICSI) vary - and explore how labs can optimize, evaluate and standardize to provide the best possible quality of treatment.
NoviSight™ Demonstration: 3D Image Analysis and Statistical Software for Organoids and Spheroids

NoviSight™ Demonstration: 3D Image Analysis and Statistical Software for Organoids and Spheroids

Experts
Hiroya Ishihara
Applications Scientist
Olympus Technologies Asia, Olympus Corporation
Three-dimensional cell culture models such as patient-derived organoids (PDO) and spheroids have increased in popularity because they can provide a 3D microenvironment that more closely reproduces in vivo conditions compared to 2D monolayer culture. Phenotypic and functional heterogeneity arise among cancer cells within the same tumor because of genetic change, environmental differences and reversible changes in cell properties. Therefore, evaluation of cell-specific responses is important for accurate prediction of drug efficacy and kinetics in vivo.
Study the Function of Stromal Cells through Intestinal Organoid Co-Culture Technology

Study the Function of Stromal Cells through Intestinal Organoid Co-Culture Technology

Experts
Dr. Ningbo Wu
Associate Professor
Shanghai Institute of Immunology, Shanghai Jiao Tong University School of Medicine
For a long period of time, intestinal mesenchymal stromal cells have been considered as a relatively simple and homogeneous group of cells. With the help of single cell transcriptomics studies, it has now been clear that these cells are quite complex and heterogeneous. However, the detailed cellular and molecular mechanisms that regulate the function of these cells remains poorly understood. Therefore, the ability to perturb and evaluate the function of these stromal cells is critical to the understanding of intestinal stem cell niche and the etiology of the inflammatory bowel diseases and colitis associated colorectal cancer.
An In Vitro System for Evaluating Anticancer Drugs Using Patient-Derived Tumor Organoids

An In Vitro System for Evaluating Anticancer Drugs Using Patient-Derived Tumor Organoids

Experts
Dr. Motoki Takagi
Professor
Medical-Industrial Translational Research Center, Fukushima Medical University
Patient-derived tumor organoids (PDOs) represent a promising preclinical cancer model that better replicates disease, compared with traditional cell culture models. We have established a novel series of patient-derived tumor organoids (PDOs) from various types of tumor tissues from the Fukushima Translational Research Project, which are designated as Fukushima (F)-PDOs. F-PDOs could be cultured for >6 months and formed cell clusters with similar morphologies to their source tumors. Comparative histological and comprehensive gene-expression analyses also demonstrated that the characteristics of PDOs were similar to those of their source tumors, even following long-term expansion in culture. In addition, suitable high-throughput assay systems were constructed for each F-PDO in 96- and 384-well plate formats.
3D Segmentation for Fluorescence Images: From Qualitative to Quantitative

3D Segmentation for Fluorescence Images: From Qualitative to Quantitative

Experts
Dr. Yu Weimiao
Head of Computational & Molecular Pathology Lab (CMPL) Agency of Science
Technology and Research
Cells are 3D functional elements in biology science and they are actively moving to perform their functions. Collective cell migration is appreciated as an important model for the understanding of the mechanism governing the cell movement in Vivo and in Vitro. It is a highly kinetic process involved in immune response, wound healing, tissue development and cancer metastasis. Recent decades have seen the fast development of various optical imaging techniques with excellent spatial-temporal resolution, dimensionality and scale. The generation of novel probes have also allowed us to acquire the movies of migrating cells with specific proteins/molecules. However, we lack of advanced solution to analyse such high-content and highly dynamic images/videos.
Prostate Cell Lineage Hierarchy and Plasticity

Prostate Cell Lineage Hierarchy and Plasticity

Experts
Dr. Dong Gao
Principal Investigator
Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences
Prostate cancer is one of the most common cancers worldwide and also the second leading cause of cancer-related death in males in Western countries. Although the majority of human primary prostate cancers have a luminal phenotype, both basal cells and luminal cells can serve as cellular origins of prostate cancer in model systems. However, the stem cell-like plasticity of defined prostate epithelial cells and the cellular origin of prostate cancer under physiological conditions have not been identified. Recently, prostate basal and luminal cell populations were both shown to be self-sustaining, and both cell types could initiate prostate cancer. However, the oncogenic transformation of basal cells requires basal to luminal cell transition. In addition, luminal cells were shown to have greater tendency to be the cells of origin for prostate cancer in some contexts.
Investigating Spheroid Architecture Using the FV3000 Confocal Microscope

Investigating Spheroid Architecture Using the FV3000 Confocal Microscope

Experts
Ms. Gency Gunasingh
Research Assistant
The University of Queensland Diamantina Institute
Phenotypic tumour heterogeneity arising due to differentially cycling cell populations has been implicated in increased therapy resistance. This phenomenon cannot be assessed in adherent cell culture, where microenvironmental conditions are homogeneous. Thus, we utilise melanoma spheroids to model the 3D tumour microenvironment including the extracellular matrix (ECM) and study spheroid structure, necrotic region, individual cell arrangement within and gene expression patterns. We achieve this by exploiting the fluorescence ubiquitination cell cycle indicator (FUCCI) system to monitor cell cycle stages as a surrogate marker for phenotypic tumour heterogeneity, tissue clearing and confocal microscopy using FV3000.
Advances in 3D Optical Imaging Technologies: An Overview

Advances in 3D Optical Imaging Technologies: An Overview

Experts
Mr. Srivats Hariharan
Manager, Applications and Marketing
Olympus Singapore
With rapid development in fluorescent proteins, synthetic fluorochromes, and digital imaging, advanced 3D imaging technologies are now available to investigators to provide critical insights into the fundamental nature of cellular and tissue functions. 3D and 4D imaging systems have become very common tools among biologists. However, there are several technical challenges and limitations in performing successful 3D and 4D imaging. Olympus has developed a wide range of 3D imaging microscopes to overcome these challenges and to satisfy the requirements of researchers across different disciplines.
3D Microscopy: Understanding the Give and Take on Instrument Performance to Enable Informed Decisions

3D Microscopy: Understanding the Give and Take on Instrument Performance to Enable Informed Decisions

Experts
Dr. Graham Wright
Acting Director
Research Support Centre (RSC), A*STAR, Singapore
Biologists have a significant toolbox at their disposal when it comes to microscopically imaging 3D samples, such as organoids. From widefield microscopy to confocal, superresolution, multiphoton and lightsheet, each have their own set of pros and cons that must be carefully considered before making an informed choice on the most suitable to address your biological question. Often a correlative approach is required, applying several techniques to address the question from different perspectives. It is also crucial to consider the method of sample preparation and optimise each of the potential steps which can include fixation, permeabilisation, labelling and mounting. Further, the images generated by all techniques can be enhanced with post-processing techniques, such as deconvolution, which can enable or help to improve subsequent image analysis and interpretation.
Tissue Optical Clearing Imaging: From In Vitro to In Vivo

Tissue Optical Clearing Imaging: From In Vitro to In Vivo

Experts
Dr. Dan Zhu
Professor
Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, Wuhan, China
Biomedical optical Imaging, as a powerful tool has been applied for observing biomedical tissue structural and functional information with high resolution and contrast unattainable by any other method. However, the high scattering of turbid biological tissues limits the penetration of light, leading to strongly decreased imaging resolution and contrast as light propagates deeper into the tissue. Fortunately, novel tissue optical clearing technique provide a way for reducing the scattering of tissue and improving the optical imaging quality. This presentation will introduce our progress from in vitro and in vivo of tissue optical clearing imaging, including developing in vitro optical clearing methods, such as FDISCO and MACS. Meanwhile, we will also demonstrate in vivo skull/skin optical clearing window for imaging structural and functional of cutaneous / cortical vascular and cells, also manipulating cortical vasculature.
Utilizing Tumoroids to Explore Anti-Tumor Immunity in Rectal Cancer

Utilizing Tumoroids to Explore Anti-Tumor Immunity in Rectal Cancer

Experts
Dr. Kasmira Wilson
Researcher
Peter MacCallum Cancer Foundation
Globally colorectal cancer is a significant public health burden. It is the third most commonly diagnosed cancer and fourth leading cause of cancer related deaths in the world. A subset of patients diagnosed with rectal cancer require neoadjuvant chemoradiotherapy (NACRT) prior to surgery. However, there is a spectrum of response to this therapy with only 10-20% of patients achieving a complete pathological response. In addition, 20-40% of patients will demonstrate no response to this treatment. There is currently no method that predicts how a patient will respond to NACRT accurately. In order to investigate the mechanisms underpinning how patients respond to therapy, patient derived tumouroids have been utilised. These personalised in vitro three-dimensional tumour models recapitulate the in vivo tumour of origin genotypically. The Ramsay laboratory (Peter MacCallum, Melbourne) has successfully co-cultured patient-matched rectal cancer tumouroids with tumour infiltrating lymphocytes (TILS) in a novel in vitro assay, preliminary data generated suggests this assay has the ability to predict the response of a patient to treatment with NACRT prior to instigation of neo-adjuvant therapy. This assay provides a pre-clinical platform that encapsulates the hosts immune response toward their tumour. However, manual analysis of the data generated from this assay is time consuming and limits the clinical utility of this platform. Machine-based learning to develop artificial neural networks capable of analysing data produced from the killing assay has been developed to automate analysis. Automated analysis utilising artificial neural networks is a feasible approach to expedite the processing of data generated from the cytotoxic killing assays and will improve the clinical utility of this platform to direct personalised patient therapy.
Converting from 2D to 3D: Bio-Techne Solutions for Your 3D Culture

Converting from 2D to 3D: Bio-Techne Solutions for Your 3D Culture

Experts
Dr. Xiaotong Cui
Field Application Specialist
Bio-Techne
Organoid and three-dimensional (3D) cell culture are emerging as pivotal systems for understanding human organ development, modeling disease, screening for drug efficacy or toxicity, and investigating personalized medicine. Usually they are derived from primary tissue, embryonic stem cells (ESCs), or induced pluripotent stem cells (iPSCs), which are capable of self-renewal and differentiation.

Culture and Quantitative 3D Imaging of Organoids: Challenges and Solutions

Experts
Dr. Anne Beghin
Assistant Professor, Research Mechanobiology Institute
National University of Singapore
Turning organoids into impactful translational models includes being able to culture them and assess those that develop robustly with physiologically relevant architecture. However, quantitative comparisons and statistical analysis at high content, which are mandatory to describe the complexity of such multicellular 3D objects are not possible owing to the lack of high-throughput 3D imaging methods. We have thus engineered a versatile High Content Screening (HCS) device to streamline all the steps of organoid culture to exploit its potential in morphogenesis understanding. Our approach comprises a new generation of versatile scaffolding cell culture multiwell chips with embedded optical components (= lighting JeWells) that enables rapid 3D imaging.

A Smarter Approach to Culturing and Nurturing Your Cells

Experts
Shogo Usui
Product Leader
In this webinar, expert Shogo Usui will discuss the current challenges in cell culture techniques and explore how the CM20 system can accelerate your research with an improved cell culture process.
现代全玻片扫描:固定样品的单细胞表型分析

现代全玻片扫描:固定样品的单细胞表型分析

Experts
Alec De Grand
产品经理
Flavio Giacobone
产品营销经理
通过本次点播网络研讨会了解最新一代的全玻片扫描仪。我们的专家将会探讨如何让现代全玻片扫描仪成为您应用的主力设备,以及如何利用多重标记技术从切片中发现更多信息。来自Ultivue的专家将帮助您了解单细胞表型如何显著改善对样品乃至固定切片的动力学理解。
3D 分析:智能软件,深层分析

3D 分析:智能软件,深层分析

Experts
Daniel Bemmerl
高端显微镜系统的区域经理
Shohei Imamura
战略项目经理
在本次网络研讨会中,我们将重点关注获得微球和基于微孔板检测的最佳统计数据。这些数据有助于量化3D模型对化合物的响应,并让诸如不同浓度不同处理的效果对比更加容易。
深度学习:开启新应用的大门

深度学习:开启新应用的大门

Experts
Kathy Lindsley
生命科学应用应用专家
Manoel Veiga
生命科学研究应用专家
Olympus Soft Imaging Solutions
Join Manoel和Kathy将探讨如何发挥深度学习的潜力应对具有挑战性的图像分析任务。

Modern Slide Scanning: Single-cell Phenotyping on Fixed Samples (Encore Edition)

Experts
Flavio Giacobone
产品营销经理
Alec De Grand
产品经理
Angela Vasaturo
Senior Field Application Scientist, Ultivue, Inc.
Get another chance to learn about the latest generation of slide scanners on November 18, when we replay our webinar with our Olympus experts Flavio and Alec. In this webinar, our experts will discuss how modern slide scanners can be a workhorse for your applications and how multiplexing can reveal much more information from a slide. With a guest expert from Ultivue, you’ll learn how single-cell phenotyping can dramatically improve the understanding of the dynamics in a sample, even if it is a fixed slide.
Olympus Discovery Summit

Olympus Discovery Summit: Advance Your Imaging | October 26–27, 2021

During two full days of virtual presentations, join your peers as they discuss the ways they are using imaging techniques to advance their research. You can also attend live product demos and tech talks from Olympus experts.

To the Diffraction Limit and Beyond: The Nanoscale Organization of Axo-Axonic Synapses | 2 p.m.

Experts
Benjamin Compans, Ph.D.
Marie Curie Research Fellow
Here we use super-resolution microscopy to decipher the precise molecular organization of axo-axonic synapses and its modifications during activity-dependent forms of plasticity.

To the Diffraction Limit and Beyond: The Nanoscale Organization of Axo-Axonic Synapses | 10 a.m.

Experts
Benjamin Compans, Ph.D.
Marie Curie Research Fellow
Here we use super-resolution microscopy to decipher the precise molecular organization of axo-axonic synapses and its modifications during activity-dependent forms of plasticity.

Light Sheet Microscopy – New multi-resolution and -color imaging methods

Experts
Irina Rakotoson
生命科学产品经理
Join Irina to discover an easy and effective method to obtain perfect multi-color, multi-resolution imaging in Light Sheet Microscopy (LSM) for 3D fluorescence imaging. In this webinar you’ll learn about the challenges of multi-color channel acquisition in LSM and how to correct chromatic shifts for clean and crisp images.
Olympus Organoid Conference: Think Deep, See Deeper | 3-Day Virtual Event | September 7-9, 2021

Olympus Organoid Conference: Think Deep, See Deeper | 3-Day Virtual Event | September 7-9, 2021

From 7 – 9 September, learn from Olympus microscope users and technology partners on how they can support your scientific research. Cell Biologists, Microscopists and Image analysis experts will share their experiences and answer your questions.

Create a Smarter Cell Culture Workflow

Experts
Joanna Hawryluk
Product Manager
In this webinar, expert Joanna Hawryluk explores how the OLYMPUS Provi™ CM20 incubation monitoring system can help improve the health and stability of cell cultures through machine learning. With the aid of AI, the CM20 monitor automatically measures cell conditions using constant analysis parameters to provide quantitative data—all while your cultures remain safely in the incubator.

Digital Image Processing: Point and Local Operation Filters (Encore Edition)

Experts
Heiko Gäthje
培训学院高级培训师
Olympus Europa
Want to get a better understanding of digital image processing techniques? Then watch our Ask the Experts webinar rerun on July 29, where we discuss how point and local operation filters can be used to control sources of error such as noise or low contrast in microscopy images. As well as exploring the mathematics behind image processing techniques such as segmentation, extraction and thresholding, our expert Heiko will also discuss the ethics of image processing – helping you judge whether specific techniques are allowed.
Depth Matters: Transforming Biology for More Realistic and Meaningful Pursuits

Depth Matters: Transforming Biology for More Realistic and Meaningful Pursuits

Experts
Dr. Gowri Balachander
Research Fellow, Translation Mechanobiology lab, National University of Singapore
Improvements to in vitro three-dimensional (3D) models are making them increasingly better at mimicking in vivo-like cellular behavior. Every tissue presents a distinct microenvironment with a unique blend of biochemical and biophysical components that dictate cellular behavior. Recreation of critical features of tissues that nurtures recapitulation of in vivo-like cellular behavior is the essence of an effective 3D model. In this webinar, through specific examples of 3D models for tissue development and cancer, we will revisit the fundamental principles of designing 3D models that can effectively recapitulate critical features of the tissues in vitro and applications of such models in mechanistic studies and drug testing. Our work also highlights the importance of 3D imaging systems, such as laser scanning confocal microscopes, which are necessary for such work.

Microscope Objectives—Where the Magic Happens (Encore Edition)

Experts
Klaus Willeke
Product Marketing Manager
Lauren Alvarenga
生命科学显微镜产品经理
Get another chance to learn about cutting-edge microscope technology on July 8th, when we replay our webinar with Olympus microscope experts Lauren and Klaus. In this webinar, the experts will discuss how objectives affect image quality and highlight why good optics are so important in complex microscope systems. The webinar also covers how progress in lens manufacturing technology is transforming the capability of advanced microscopy systems, and explores how versatile new lenses are enhancing applications including whole slide imaging, super resolution systems, and laser scanning microscopy.

Three-Dimensional High-Throughput Image Analysis of Patient-Derived Organoids and Spheroids

Experts
Hiroya Ishihara
Applications Scientist
Olympus Technologies Asia, Olympus Corporation
Organoids and spheroids can more faithfully reproduce in vivo conditions, and imaging-based analysis can monitor cell-specific responses with high spatial resolution. Therefore, we have been developing imaging-based three-dimensional analysis and drug evaluation methods using patient-derived cancer organoids and spheroids.
Olympus Discovery Summit—Looking Forward: A New Era of Research

Olympus Discovery Summit—Looking Forward: A New Era of Research

The Olympus Discovery Summit, held in April, featured microscopists and imaging experts sharing about advances in technology and research. Experience or relive this free three-day virtual event by watching the webinars on demand. Hear from your peers and learn about topics, including best practices for a post-pandemic workplace, emerging technologies, next-generation models for research, and more!

Digital Image Processing Part 2: Advanced Image Processing Filter

Experts
Heiko Gäthje
培训学院高级培训师
Olympus Europa
In our first webinar on this topic, we learnt that images taken with a light microscope are never quite a perfect reflection of our samples. There are always sources of error, and as we can only minimize such errors, the raw data of an imaging experiment will often need some digital image processing before final image analysis.
ICSI—Past, Present & Future

ICSI—Past, Present & Future

Experts
Dr. Hrishikesh Pai
Medical Director, Bloom IVF Group
In this webinar, Dr. Pai will be sharing on what’s new in the management of male infertility with special focus on Intra Cytoplasmic Sperm Injection (ICSI). He will be describing the latest diagnostic modalities in male infertility especially sperm DNA fragmentation, genetic testing, cryotozoospermia and microsurgical TESA in cases of non-instructive azoospermia.

Microscope Objectives—Where the Magic Happens

Experts
Ganesh Kadasoor
Olympus Medical Systems India Ltd应用专家
Wei Juan Wong
奥林巴斯新加坡高级产品专员
Olympus Soft Imaging Solutions
In this webinar, Olympus microscope experts Ganesh and Wei Juan discuss the importance of good optics in a complex microscope system and what optical features are important for high final image quality.

Microscope Objectives—Where the Magic Happens

Experts
Klaus Willeke
Product Marketing Manager
Lauren Alvarenga
生命科学显微镜产品经理
In this webinar, Lauren and Klaus would like to emphasize the importance of good optics in a complex system like a microscope. They will discuss different aspects which are important for the final image quality.

利用近红外激光扫描共聚焦显微镜实现更深层观察

Experts
Chunsong Yan
Process Systems业务发展经理
Olympus Australia & New Zealand
Srivats Hariharan
奥林巴斯新加坡公司产品应用经理
想要在样品中添加更多荧光标记?想要以高分辨率实现样品的更深层成像?想要以尽可能低的光毒性进行活细胞成像?
High-Content Screening: Customized Analysis Made Easy

High-Content Screening: Customized Analysis Made Easy

Experts
Manoel Veiga
生命科学研究应用专家
Olympus Soft Imaging Solutions
Shohei Imamura
战略项目经理
In this webinar, Manoel and Shohei, our deep learning and high-content screening experts, will introduce the scanR system’s assay builder. The scanR system is Olympus’ dedicated high-content screening platform with a unique sample navigation approach and analysis inspired by flow cytometry.
Digital Image Processing: Point and Local Operation Filters

Digital Image Processing: Point and Local Operation Filters

Experts
Heiko Gäthje
培训学院高级培训师
Olympus Europa
Takeo Ogama
高级产品和策略规划师和产品经理
Images captured with a light microscope are never true representations of the specimen; there are always sources of error that must be controlled. In this webinar, we will discuss how these sources of error can be managed.
Live Cell Super Resolution Imaging: The Big Picture of Small Things

Live Cell Super Resolution Imaging: The Big Picture of Small Things

Experts
Chunsong Yan
Process Systems业务发展经理
Olympus Australia & New Zealand
Lauren Alvarenga
生命科学显微镜产品经理
Stefan Marawske
高端生命科学系统高级销售专员
Olympus Europa
In this webinar, Olympus imaging experts Stefan, Lauren, and Chunsong will discuss how to more easily create super resolution images.
Light Sheet Microscopy for Deeper Insight into Life

Light Sheet Microscopy for Deeper Insight into Life

Experts
Irina Rakotoson
生命科学产品经理
In this webinar, you’ll learn how the Alpha3 light sheet microscope combines very thin optical sectioning and high-quality Olympus optics for high-resolution 3D imaging of both live and fixed biological samples.
FluidFM: A Novel Approach to CRISPR Gene Editing by Direct Intra-Nuclear Delivery

FluidFM: A Novel Approach to CRISPR Gene Editing by Direct Intra-Nuclear Delivery

Experts
Paul Monnier
生命科学应用科学家
In this webinar, Paul Monnier from Cytosurge will show you how to overcome one of the biggest challenges in gene editing: delivery of the genetic material into the nucleus. Paul will discuss the FluidFM nanosyringe that can gently pierce the nuclear envelope to deliver reagents directly into the nucleus.
Multiplexing and Deep Tissue Imaging with Near-Infrared Confocal Laser Scanning Microscopy

Multiplexing and Deep Tissue Imaging with Near-Infrared Confocal Laser Scanning Microscopy

Experts
Bülent Peker
高级产品营销经理
Olympus Europa
Rebecca Bonfig
共聚焦显微镜产品经理
In this webinar, you’ll learn about fluorescence multiplexing and deep tissue imaging using near-infrared (NIR) laser light.
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