奥林巴斯的超分辨率技术

奥林巴斯的超分辨率（OSR）技术使用起来迅速便捷，可以对细胞内深达100微米的区域进行成像操作，而使用其它超分辨率模式则很难达到如此深的区域。使用常规荧光染料即可在120 nm的分辨率下，采集到各种样品内部细胞结构的活细胞超分辨率图像。

Spinning Disk Delivers Bright Live Cell Imaging

The high-sensitivity model equipped with SoRa disk realizes brighter super resolution image acquisition thanks to the spinning disk with microlens in the confocal pinhole. Each confocal pinhole enables you to image with lower laser power, reducing photobleaching and phototoxicity in your sample while enabling bright super resolution images.

Brightness comparison Left: Standard model / Right: High-sensitivity model Button

Principle of CSU-W1 with SoRa disk Configuration of CSU-W1 with SoRa disk

In regular confocal microscopes, image formation is a product of the illumination point spread function (PSF) and detection PSF. Looking at the image formation on the pinhole at position D from the optical axis, it’s the product of the illumination PSF and detection PSF, and we can see that information from position D/2 from the optical axis is transmitted but not resolved. To correct this, a microlens is fitted in the pinhole, and the individual focal points projected onto the pinhole are optically reassigned to the center, creating an ideal image and increasing the brightness and resolution. This process makes the resolution nearly equal to that of an ideal confocal microscope in which the pinhole has been reduced to an infinitesimal size.

_{Reference: T. Azuma and T. Kei, "Super-Resolution Spinning-Disk Confocal Microscopy Using Optical Photon Reassignment, " Opt. Express 23, 15003-15011 (2015).}

降低的光毒性

实时控制器（U-RTCE）可使激光器照明和摄像头成像达成微秒级的同步，以降低光漂白和光毒性，从而可在复杂的试验中使细胞保持健康的状态。

在延时成像过程中，在温度、湿度及其它方面的任何微小变化都会使样品偏离焦点。Z轴漂移补偿系统（IX3-ZDC2）使用一个具有低光毒性的红外激光器，辨别样品平面，并调整焦点位置，以获得清晰的延时图像。连续自动聚焦功能可用于放置在玻璃和塑料器皿中的样品。

Learn more about the U-RTCE

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柯蒂氏（螺旋器）器官中内毛细胞的静纤毛和动纤毛（肌动蛋白：橙色，微管蛋白：绿色）

_{图像数据由Hatsuho Kanoh1、Toru Kamitani1&2、Hirofumi Sakaguchi2和Sachiko Tsukita1提供。
1前沿生物科学研究生院和医学研究生院，大阪大学。
2耳鼻喉和头颈外科系，京都府立医科大学。}