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Imaging Multiple Subcellular Structures at the Nanometer Scale in 3D

During this webinar, Nicolas will show you how fast and easy it can be to obtain high-quality, reliable, and understandable single-molecule images of your samples with 15 nm resolution in 3D. He will go through the entire imaging workflow—from sample preparation to data analysis—to show you what the single-molecule local microscopy (SMLM) community has made possible, what the common mistakes are, and how Abbelight’s tools and support can make the imaging and analysis journey much easier.

Presenter:

Dr. Nicolas Bourg, Chief Technical Officer and Co-Founder of Abbelight

FAQ

Webinar FAQs | Imaging multiple subcellular structures at the nanometer scale

How long does it take to acquire 3D multicolor nanoscopy images?

The spectral demixing strategy means that acquisition of three-color images typically takes less than one minute for a standard field of view in 3D, and a few minutes for larger fields of view.

How difficult is it to prepare nanoscopy structures?

Sample preparation requires only simple modification of the standard preparation procedure, such as changing the concentration of the fixative (e.g., PFA) or increasing the antibody concentration. It only requires fine tuning of your own protocol. 

Imaging Multiple Subcellular Structures at the Nanometer Scale in 3D

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Ask the Experts: Imaging multiple subcellular structures at the nanometer scale in 3D

During this webinar, Nicolas will show you how fast and easy it can be to obtain high-quality, reliable, and understandable single-molecule images of your samples with 15 nm resolution in 3D. He will go through the entire imaging workflow—from sample preparation to data analysis—to show you what the single-molecule local microscopy (SMLM) community has made possible, what the common mistakes are, and how Abbelight’s tools and support can make the imaging and analysis journey much easier.

FAQ

Webinar FAQs | Imaging multiple subcellular structures at the nanometer scale

How long does it take to acquire 3D multicolor nanoscopy images?

The spectral demixing strategy means that acquisition of three-color images typically takes less than one minute for a standard field of view in 3D, and a few minutes for larger fields of view.

How difficult is it to prepare nanoscopy structures?

Sample preparation requires only simple modification of the standard preparation procedure, such as changing the concentration of the fixative (e.g., PFA) or increasing the antibody concentration. It only requires fine tuning of your own protocol. 

Experts
Dr. Nicolas Bourg
Chief Technical Officer and Co-Founder of Abbelight

Hello. I’m Dr. Nicolas Bourg, chief technical officer (CTO) and co-founder of Abbelight and I’m your designated expert for single molecule localization microscopy (SMLM) – as known as nanoscopy – which includes techniques like PALM, dSTORM, SPT-PALM and DNA-PAINT.

I’m an optronic engineer by training, and I did a Ph.D. in biophotonics at the Paris-Saclay University, working on a unique 3D nanoscopy technique with unprecedented resolution. With my research team, we decided to share all the knowledge I acquired during my PhD and we created Abbelight: a startup that aims to make nanoscopy even more powerful and fully accessible to any biologist without advanced microscopy training. My job is to answer all your questions about nanoscopy, so please get in touch.

Imaging Multiple Subcellular Structures at the Nanometer Scale in 3DSep 22 2021
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